Center for Phage Technology

Immunogenic Streptococcus equi cell surface proteins identified by ORFeome phage display | mSphere This work utilized an ORFeome phage display platform to systematically identify antigenic epitopes produced by Streptococcus equi subspecies equi (S. equi), an important equine pathogen and the causative agent of horses strangles. Three major S. equi surface proteins were identified: a novel proline-rich repeat domain protein, a serine peptidase, and the M-like protein SeM. The proline-rich repeat protein and serine peptidase were confirmed to be immunogenic in horses with strangles, and their sequences were shown to be conserved in global S. equi genomes, in contrast to their diversity in S. equi subsp. zooepidemicus. With the well-characterized S. equi immunogenic protein SeM, this paper identified an immunogenic region outside of the reported critical IgG-binding region. This work provides novel insights to the understanding of the S. equi immunogenic proteins and provides peptide regions that could serve as vaccine candidates against S. equi or as diagnostic markers to specifically identify S. equi infections.

Our newest publication in collaboration with @tubsmedbiotech.bsky.social @tu-braunschweig.de on S. equi immunogenic surface proteins identified by ORFeome phage display journals.asm.org/doi/10.1128/...

The Lysis Cassette of Jumbophage phiKZ Pseudomonas jumbophage phiKZ is prominent because of the proteinaceous nuclear structure that is formed during the infection cycle, conferring resistance of the replicating phage DNA to anti-phage res...

And we also characterized the lysis cassette of the Jumboohage phiKZ www.biorxiv.org/content/10.1...

The Lysis Genes of the Paradigm Phage SP6 | PHAGE Background: The Salmonella podophage SP6 has been extensively studied as a divergent relative of T7 and as a prototype for therapeutic applications. Two decades ago, it underwent two comprehensive gen...

We recently revised the genome of the paradigm phage SP6 and characterize its lysis genes: www.liebertpub.com/doi/10.1177/...

The recipient of the 2025 Thomas Patterson Graduate Student Fellowship is Michael Awuah from @jrrmicro.bsky.social Lab for his work decoding the molecular basis of phage lysis inhibition. We are grateful for the generous support of this award by Drs. Tom Patterson and @superbug-slayer.bsky.social!

We welcome the visit of Dr. Michael Hust and Philip Heine @tu-braunschweig.de @tubsmedbiotech.bsky.social and appreciate their collaboration on ORFeome phage display projects!

Phage Therapy for Multidrug-Resistant Bacterial Infections: Past and Future February 10, 2025 at 12:30 pm US ESTThe session will begin with a presentation from our guest speaker, Dr. Jason Gill, providing an overview of the science and key lessons from bacteriophage developme...

@jasonjgill.bsky.social is talking at N=1 Collaborative Seminar TOMORROW 12:30pm EST on "Phage Therapy for Multidrug-Resistant Bacterial Infections: Past and Future" , featuring some of the CPT work. www.n1collaborative.org/post/phage-t...

Fine-tuned spatiotemporal dynamics of DNA replication during phage lambda infection | Journal of Virology Temperate bacteriophages, such as lambda, incorporate environmental cues including host abundance and nutrient conditions to make optimal decisions between propagation and dormancy. A higher phage-to-...

Read our recent paper by @yu-zihao.bsky.social using a fluorescence reporter system that facilitates the visualization of individual copies of phage DNA throughout the phage life cycle, along with the lysis–lysogeny reporters. journals.asm.org/doi/10.1128/...

Bonus to our new followers: Our phage database prototype is now online:
dbop.phagetech.org. Last updated by @jrrmicro.bsky.social, currently only holds spanin proteins.

Phage DNA Extraction, Genome Assembly, and Genome Closure Bacteriophages, or more simply phages, are currently experiencing a renaissance in life science research for their roles in natural microbial communities, their potential use as antimicrobials, and bi...

Some of our phage protocols including phage DNA extraction, genome sequencing and assembly, and very importantly, genome closure from the assembled contig, are now available online!
link.springer.com/protocol/10....

Phage Milagro: a platform for engineering a broad host range virulent phage for Burkholderia - PubMe... The <i>Burkholderia cepacia</i> complex (Bcc) causes life-threatening respiratory tract infections in persons with cystic fibrosis (CF). In CF patients, end-stage pulmonary disease often requires lung...

Our newest paper on engineering Burkholderia phage is out! pubmed.ncbi.nlm.nih.gov/37943040/