A new preprint from the lab. I won't do a tweetorial until this is peer reviewed, but I think it's a banger, led by
@JedNzy. It's about Rubisco and what chaperones are really for. He's on the market, get him while you can. www.biorxiv.org/content/10.1...
Excited about #Ubiquitin and protein degradation?
Have a look at my recent talk at the @danafarber.bsky.social Targeted Protein Degradation Webinar Series now out on YouTube. Here, I talk about my recent publication (see Blue-torial below)
www.youtube.com/watch?v=Cpg0...
New paper from the lab from Sriram Garg in my group. We introduce a general substitution matrix for structural phylogenetics. I think this is a big deal, so read on below if you think deep history is important. academic.oup.com/mbe/advance-...
@amoebadiscovery.bsky.social made an iteresting interview with me about #heliozoa and #centrohelids - I think these organisms are really important, understudied and gorgeous ๐ Also lots other intresting reading and beatiful pictures! #protistsonsky link: tinyurl.com/adiscovery1
Congrats!! ๐ฅณ
Super excited to share our latest work on deciphering the #Ubiquitin Code
โ๐จ๐ฏ๐ถ๐ฅ๐๐๐ ๐ฑ๐ฒ๐ฐ๐ถ๐ฝ๐ต๐ฒ๐ฟ๐ ๐ฝ๐ฟ๐ผ๐๐ฒ๐ฎ๐๐ผ๐บ๐ฎ๐น ๐ฑ๐ฒ๐ด๐ฟ๐ฎ๐ฑ๐ฎ๐๐ถ๐ผ๐ป ๐ฐ๐ผ๐ฑ๐ฒ ๐ผ๐ณ ๐ต๐ผ๐บ๐ผ๐๐๐ฝ๐ถ๐ฐ ๐ฎ๐ป๐ฑ ๐ฏ๐ฟ๐ฎ๐ป๐ฐ๐ต๐ฒ๐ฑ ๐48 ๐ฎ๐ป๐ฑ ๐63 ๐๐ฏ๐ถ๐พ๐๐ถ๐๐ถ๐ป ๐ฐ๐ต๐ฎ๐ถ๐ป๐โ
@cp-molcell.bsky.social
1/8
www.cell.com/molecular-ce...
Credit: Margot Riggi (@margotriggi.bsky.social), Max-Planck Institute of Biochemistry (@mpibiochem.bsky.social)
Asgard archaea have actin - but what about microtubules? Where do they come from? ๐ง Our new paper www.cell.com/cell/fulltex... by @xujwet.bsky.social & @florianwollweber.bsky.social, in collaboration with the Schleper & Wieczorek labs, describes tiny Asgard microtubules! #TeamTomo #ArchaeaSky 1/6
Excited to share our latest work, led by the incredible @automnenine.bsky.social (now an Ass. Prof. at Princeton)! We uncover key insights into the retrotransposition mechanisms of LINE-1 ORF2p, the enzyme responsible for writing a significant portion of our genome.
www.science.org/doi/10.1126/...
The Foldseek webserver for fast protein structure searches now features a Sankey tree taxonomy visualization and filter, allowing to subset hits by clades. Developed by my talented student @sunjaelee.bsky.social. Try it out!
๐ search.foldseek.com
Excited to share the (hard) work of @zawarshah.bsky.social in its final form: www.nature.com/articles/s41...
A lot of insights into RNA Polymerase selectivity at snRNAs promoters !! As these promoters are often used for heterologous expression of RNAs (ie guide RNAs), you better know how they work
Exciting 3-year postdoc opportunity with @carter-lab.bsky.social within a programme aimed at identifying the links between microtubule motors and axonal cargos.
Read more about the role here:
www.nature.com/naturecareer...
Apply by 30 DEC
#ScienceJobs #CambridgeJobs #PostdocJobs #GenomeEngineering
We discovered 3 RNA families that form homo-oligomeric RNA-only complexes! Studying nature continues to expand my wildest imagination. Read about it: doi.org/10.1101/2024...
Vivian Wu, Svetlana Shabalina, Hyunbin Lee, Grace Nye, Eugene Koonin, Alex Gao, Rhiju Das, Wah Chiu
Excited to share our new work on the earliest steps of Pol-III transcription: We provide structural and kinetic insights into tRNA promoter engagement by yeast transcription factor TFIIIC !
Cool collaboration with the Mรผller and Eustermann Labs @embl.org !
academic.oup.com/nar/advance-...
This is my first post here :) Our TFIIIC story continues with our latest work in NAR (academic.oup.com/nar/advance-...). Using cryo-EM and single-molecule fluorescence microscopy (smFM), we explored unanswered questions in our human TFIIIC structure. Want to see the updated TFIIIC structure?
First paper of our lab today @natureportfolio.bsky.social by Nussi!
We provide a real-time movie on how #transcription and #translation cooperate using #single-molecule FM: we find long-range #ribosome/ RNAP communication mediated by #RNA looping!
@embl.org #RNAbiology #RNASky
t.co/a24Xxcxdo0
Really nice to see our latest work on Sox/POU origins covered in detail in the @washingtonpost.com by Kasha Patel, the ๐ฆ choano-mouse๐ is getting the love it deserves! www.washingtonpost.com/climate-envi...
This scheme shows the cargo adaptor FHF in the middle with arrows pointing to distinct microtubule and actin-based motors (dynein-dynactin, KIFC1, KIF1C and myosins V, IX and X as well as Tropomyosin 1). It also shows a link to Rab5 marked early endosomes. The hypothesis is that FHF coordinates multi motor binding at the early stages of endocytosis. The PhD project would address the hierarchy of motor binding and how these motors coordinate to allow the newly formed vesicle to transition from the actin to MT cytoskeletal network.
If youโre interested in how molecular motors coordinate to power intracellular trafficking events (e.g. endocytosis), Iโve got a PhD position available to study this at vibrant Bristol uni. In this project, we will capture highly dynamic events by #cryoEM, single molecule imaging & cell biology. ๐ฌโ๏ธ๐งช
Many thanks to our collaborators, especially to my joint-first authors Ya Gao and Daisy Tan and to Ralf Jauch and Alex de Mendoza, and of course Georg Hochberg. This was an amazing collaboration.
This research provides new perspectives on the evolution of multicellularity - a major transition in life's history. It could have implications for developmental biology and regenerative medicine!
(9/n)
Our findings suggest that the evolution of animal stem cells might have involved repurposing pre-existing molecular tools, rather than inventing entirely new mechanisms.
(8/n)
Interestingly, while we found POU proteins in choanoflagellates too, they couldn't induce pluripotency. This suggests that POU factors needed further adaptations to function in animal stem cells.
(7/n)
This means that these ancient proteins have all the molecular features necessary to act as pioneer factors and induce stemness in mammalian cells. Pretty amazing for a 700-million-year-old protein!
(6/n)
Using ancestral sequence reconstruction, we "resurrected" ancient metazoan Sox proteins. These reconstructed proteins could also induce pluripotency in mice!
(5/n)
But here's the really cool part: choanoflagellate Sox proteins can replace mouse Sox2 in reprogramming mouse cells into induced pluripotent stem cells (iPSCs)!
(4/n)
We found Sox-like proteins in single-celled relatives of animals (choanoflagellates). Surprisingly, these ancient proteins can bind DNA just like their mammalian counterparts! ๐งฌ๐
(3/n)
Our study reveals that key proteins involved in animal stem cell regulation (SOX and POU transcription factors) are much older than previously thought! They actually predate the origin of animals, which evolved over 700 million years ago.
(2/n)
Excited to share my first paper as a postdoc with @KaHochberg @mpi_marburg! We've made some fascinating discoveries about the evolution of animal stem cells in colab with @jauchr and @deMendoza_Alex. ๐งฌ๐ฌ
Link: www.nature.com/articles/s41... (1/n)
