Jon Daniels

Gratifying to have been a part of the tech dev behind this (specifically high-res cleared tissue light sheet microscopy)

Of course I'm happy to share Zemax BB file, plots, etc.

Specs continued:
- 400-1300 nm internal coatings
- designed for light sheet but possibly also useful for 2P: at NA 0.8 illumination, FOV 1.0mm up to ~1150nm
- mechanically efficient; co-focuses with 54-12-8
- parfocal length 83 mm
- M32x0.75 threads

I'm very happy with the new ExM objective design that I've been working on with Special Optics

Now looking for pre-orders using kickstarter model.

Key specs:
- NA 1.0 water-dipping
- FOV 1.1 mm
- WD 5.4 mm
- EFL 7.5 mm
- flat field
- chromatic corrections optimized for 500-750 nm

Bill Gates would be on that list if he hadn't given away more.

Cool use for the ASI single-objective light sheet at MPI-CBG

I can't take credit for the passive crossbrace concept and design -- that would be @amsikking.bsky.social and friends -- but we have them to sell.

+1 for Zulip, I appreciate having topics within channels to keep things organized. Free plan and ability to self-host.

Maybe the day will come when the Big4 will release black box files... kudos to @pacificoptica.bsky.social and Thorlabs (and of course ASI) for doing that for their objectives.

I have noted the following, likely gathered from Nikon reps:

OFN22: Water Dipping, Super Fluor, TIRF, Plan Achromats, Achromat, some WI

OFN25: Plan Fluor, Plan Apo Lambda, Plan Apo VC, some Lambda S, some WI

Without a spec for what exactly the FN means it's only a hint.

I made a mistake on axial color, it's actually only 1.6um between 480nm and 680nm, and 0.1um between 488nm and 561nm. So axial color isn't too far from the diffraction limit.

83mm parfocal, M32 threads

One compromise made is axial color: 7um axial shift between 480nm and 680nm (2.7um between 488nm and 561nm)

Email me if you want the Zemax black box or some specific simulation result. jon@asiimaging.com

I'm working on an objective lens for expansion microscopy. I have a candidate design to share. Looking for feedback before we finalize things.

NA 1.0 water-dipping
5.5mm WD
1.1mm FOV, plan-corrected
8mm EFL ("25x")
corrected in visible with exception noted below
minimum mechanical profile

I highly recommend this course as an introduction to microscopy.

Our snouty v3 (AMS-AGY v3) objective arrived today! Looking forward to getting it installed on our single objective light sheet system very soon...

Do you know to what extent other objective lenses are intrinsically telecentric?

Particularly comparable ones like Nikon 40x/0.8, Olympus 20x/1.0 W, Nikon 25x/1.1 W (which isn't plan-corrected but still widely used in light sheet).

Related, I'm curious to what extent other objective lenses are intrinsically telecentric.

Particularly comparable ones like Olympus 20x/1.0 W, Nikon 25x/1.1 W (which isn't plan-corrected but still widely used in light sheet), and Nikon 40x/0.8 W.

Designing the whole optic train together makes sense for OPM or similar applications. It can be done with the BB model (which we will give out).

Primary use as detection lens with expanded samples for light sheet with separate illumination path, or for epi-fluorescence including confocal.

Thank you, good point. If the objective is not intrinsically telecentric, can you still make a combined telecentric system with it, e.g. adjusting the axial position of the galvo so that the scanning is self-parallel?

I'm working on a new water-dipping objective targeting expansion imaging. Targeting the following specs:

5mm WD
NA 1.0
1.2mm FOV, plan-corrected
8mm EFL ("25x") but this is flexible
well-corrected in visible
minimum mechanical profile

Optics question: is there any reason to make it telecentric?

AMS-AGY v3 objective replica

The ASI machine shop made a nice replica model of the AMS-AGY v3 objective for us to have in the #CellBio2025 booth. The first of the real ones will be ready by the end of the year.

If you are at #CellBio2025 stop by and say hello at booth 1101

Smaller fan angles were better for our application. Because Laserline only had 1° and 5°, we had a special 2° one made (which we can sell if it's helpful to others).

I suggest using a slit for only modest adjustments in NA because of light efficiency.

Cells dynamically adapt their nuclear volumes and proliferation rates during single to multicellular transitions Tumour development and progression are associated with biophysical alterations that manifest across multiple spatial scales, from the subcellular to multicellular tissue scale. While cells can dynamic...

Great to see the ASI single-objective light sheet used for long-term imaging of tumor cells to study the transition between single-cell and multi-cellular spheroids: www.biorxiv.org/content/10.1...

Pan-ASLM: a high-resolution and large field-of-view light sheet microscope for Expansion Microscopy Expansion microscopy, a super-resolution fluorescence microscopy technique in which samples are expanded up to ∼8,000 times (after 20-fold expansion) their original volume, places high demands on the ...

BTW these objectives generally work for water/ExM though not specifically optimized for it. For instance the 54-12-8 -- NA 0.64 in water -- was used as the illumination objective for ASLM imaging of pan-ExM samples in www.biorxiv.org/content/10.1...

of course

In the past this (and the glycerol one) was available for purchase only with Olympus/Evident confocal systems, not to homebuilders. Not sure if they have changed their policy.

for cleared tissue see these four objectives with different NA/WD/FOV tradeoffs: asiimaging.com/docs/multi-i...

Note this would be a dipping objective and basically a regular microscope objective lens, compared to the specialized ExaSPIM lenses which are awesome but unconventional optically.

I have some thoughts in this direction... I'm thinking NA 0.75 or NA 0.8 with >10mm WD.

I'm hoping to talk with researchers at Seeing Is Believing and SfN (or by email/DM) and then start the detailed design.

Please reach out to me jon@asiimaging.com.