It was super nice to be part of such challenging undertaking in integrative structural biology. For anyone interested in a mechanistic understanding of mRNA decay via CCR4-Not and IDRs in general, this is for you!
This work is now officially published
@natsmb.nature.com
with some fun new analyses included in the revised version. Congratulations to all the authors! @connyyu.bsky.social @rappsilber.bsky.social @lrsinn.bsky.social @fjoreilly.bsky.social www.nature.com/articles/s41...
The "641 Hz" in the 750 Da/s-5 Da Q1-ZT Scan DIA method corresponds to the effective TOF-MS/MS scan rate after correcting for overheads from data binning, TOF-MS, and some other processes SCIEX knows more about
Hi Hamish,
to the best of my knowledge, "dwell time" here is defined as the time it takes to transfer ions of a certain m/z in the Q1. We tried to match ZT Scan's dwell time to ZenoSWATH's MS2 accumulation times for a fair comparison of both methods.
Indeed, 0.67 ms is the duration of a Zeno pulse.
Beyond support by SCIEX, we acknowledge funding from the BMBF, MSCoreSys, the SFB TRR186 and @erc.europa.eu.
A special Thanks to everyone involved from the Ralser Lab (ralser.group), SCIEX @sciex.bsky.social, as well as @vadim-demichev.bsky.social and @christophmessner.bsky.social.
We are super thrilled to see ZT Scan DIA leading to insight from miniscule proteome samples, in clinical proteomics and functional proteomics studies that depend on highly precise protein quantification from large sample series.
The meticulous development with our collaborators from SCIEX (@stevetate_absx and the magnificent team) was worth the effort, seeing gains between 30-40% in Protein IDs and improved quantitative performance in high-throughput, as well as with low-input proteomics applications.
Combining the benefits of ZenoTrap and Q1 scanning
I am excited to see our performance assessment of the successor of Scanning SWATH on the Zeno TOF7600+ mass spectrometer - ZT Scan DIA - pre-printed (www.biorxiv.org/content/10.1...
