Nicholas Schnicker

Figure 1. Cryo-EM of abundant protein complexes in native membranes.

Figure 2. Cryo-EM of membrane proteins in vesicles.

Figure 3. 3D reconstruction of V-ATPase in native synaptic vesicle membranes.

Figure 4. Generation of membrane vesicles for structure determination of proteins in their native lipid bilayer.

I've written a review on what I think is an extremely exciting direction in cryo-EM:

Cryo-EM of endogenous membrane proteins in their native lipid bilayer

Open access in Quarterly Reviews of Biophysics:
doi.org/10.1017/S003...

📣 The 2026 Midwest DNA Repair Symposium is coming to Kansas City (registration is open)

🗓 May 30–31, 2026

✨ Talks will be selected from abstracts, with a strong focus on trainees and new investigators.

Please RT & share with your labs!
🔗 sites.google.com/view/kumc-mi...

As construction of the room for our new Glacios CryoEM comes to an end - here's a quick look at the process.
*Not pictured: lots of people to thank, many meetings, phone calls, challenges, delays, and teamwork.
I learned a lot during this process - now I'm ready for installation and data collection!

Base excision repair in chromatin: A tug-of-war for DNA damage Base excision repair (BER) is a genome surveillance pathway responsible for repairing DNA base lesions distributed throughout the chromatinized eukary…

🎉 Joint paper from the Freudenthal and @tmweaver.bsky.social Labs!
Abbey Vito’s first publication discusses a tug-of-war between BER enzymes and chromatin for access to damaged DNA, a battle central to genome stability.
Built on Tyler's foundational work! tinyurl.com/ywkt2bt5

Register for the Oct 30 Virtual Office Hour on Structure Summary Pages Learn how a exploring a single PDB structure at RCSB.org can lead to visualizations, analyses, and searches across the archive

Happening this week! Register for the Thursday October 30 Virtual Office Hour on Structure Summary Pages
Learn how a exploring a single PDB structure at RCSB PDB can lead to visualizations, analyses, and searches across the archive

Chris Russo and Anastasiia Gusach have developed a new method to prepare #cryoEM samples that avoids protein damage during freezing

Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...

#LMBResearch

Another one!

Great info here!

In another preprint from the @brianhie.bsky.social Lab and @synbiogaolab.bsky.social, they introduce Germinal, a generative AI system for de novo antibody design.

Germinal produces functional nanobodies in just dozens of tests, making custom antibody design more accessible than ever before.

Love this pipeline! Congrats to all involved!

Welcome! You are invited to join a webinar: Soluble Proteins in Focus: Preparing for Cryo-EM Success. After registering, you will receive a confirmation email about joining the webinar. Are you using cryo-EM to look at small soluble proteins? Join Dr. Claudio Catalano, a Senior Scientist at NanoImaging Services, to get expert advice on how to set your project up for success. He wil...

🧪 Skip the trial and error for structure determination of soluble proteins. Join the #webinar 10/8 for a gene-to-structure case study of CYP3A4, a 57 kDa cytochrome P450 monooxygenase, solved to 3.1 Å by #cryoEM.

nimgs.zoom.us/webinar/regi...

SCIMaP - Impacts of Federal Cuts to Science and Medical Research View Projected Impact of the FY2026 Budget Cuts to the NIH

Major update from #SCIMaP...

FY26 House district level scorecards.

Our FY26 budget analysis tab includes clickable district info, breaking down the expected economic and job loss given the White House's budget proposal that would slash NIH research funding by >40%.

scienceimpacts.org/fy26

Also curious what the creators @martinpacesa.bsky.social and others use for visualizing results?

Protein Structure Viewer Upload and interactively visualize multiple Protein Data Bank (.pdb) structures.

Just made my first webapp mostly for analyzing structures from #BindCraft
👉🏼 pdb-viewer.lovable.app

It’s bare 🦴 at this point but curious if others find something like this useful and features they’d like?
🧬🧶

We’ve refreshed the AlphaFold Database Entry Pages!

Explore the cleaner design, improved 3D viewer, and easier access from any device 👇
www.alphafold.ebi.ac.uk/entry/P04637

Or read more👇
www.ebi.ac.uk/about/news/u...

#AlphaFold #Bioinformatics #UXForScience
@pdbeurope.bsky.social

Affinity-ligand purification of native human low-abundance multi-protein complexes for structure determination www.biorxiv.org/content/10.1101/2025.07.31.667873v1 #cryoEM

CryoSift – An accessible and automated CNN-driven tool for cryo-EM 2D class selection

Check out our new preprint from the @landerlab.bsky.social, Stagg-Lab and Cianfrocco-Lab: CryoSift - An accessible and automated CNN-driven tool for cryo-EM 2D class selection www.biorxiv.org/content/10.1...
Happy testing: cryosift.org #cryoEM #CNN

AlphaFold developer Google DeepMind to fund CASP as NIH funding falls short Protein structure prediction contest CASP gets temporary funding from Google DeepMind as NIH grant runs out.

NEW from me at STAT:

We've learned that DeepMind is going to fund CASP, the protein structure competition that brought Google DeepMind's #AlphaFold to prominence (and a Nobel Prize), as its NIH funding runs out.

More @statnews.com:
www.statnews.com/2025/07/21/c...

🖥️🩺🧪🧬 #bioML

Maybe not necessary in your case with high seq identity but also nice since it lets you show the secondary structure alignment as well

Disorder meets its match Designed protein pockets recognize intrinsically disordered protein regions

Designed protein pockets recognize intrinsically disordered protein regions @science.org
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
www.statnews.com/2025/07/17/n...

Takes some fiddling but this has worked well.

espript.ibcp.fr/ESPript/ESPr...

SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.

CASP is the main reason the protein structure prediction technology and research field advanced over the last 30 years. And the main reason AI based methods have been accepted and widely applied in biology. So shortsighted of NIH to postpone or even halt funding. John Moult is a scientific hero.

We have written up a tutorial on how to run BindCraft, how to prepare your input PDB, how to select hotspots, and various other tips and tricks to get the most out of binder design!

github.com/martinpacesa...

🧪 We're beta testing - and we want your thoughts!
Try the redesigned PDBe entry pages & let us know:
🔸 What’s working?
🔸 What’s confusing?
🔸 What features do you miss?

💬 Feedback form: tinyurl.com/pdbe-beta-form

#PDBeBeta #Bioinformatics #Biochemistry #Science #UserVoice

CCP4 Study Weekend 2025 - Using software, AI and other methods to advance macromolecular models. - YouTube Recordings of talks from the CCP4 Study Weekend 2025 held at EMCC, Nottingham between 7-9 January 2025. The theme of the meeting was "Using software, AI and ...

The videos for the recent CCP4 Study Weekend 2025 are now available on YouTube.
www.youtube.com/playlist?lis...

Fantastic day sharing knowledge with the brilliant researchers at NIH who valiantly work to advance the frontiers of science for the cause of improving human health. Thank you again for your service 🙏 🇺🇸

a cartoon character is pointing at a potato with the words `` come on do something '' written above it . ALT: a cartoon character is pointing at a potato with the words `` come on do something '' written above it .

Waiting for binder design results…
#denovo #proteindesign

The cryo-em core at kumc is looking for a lab manager. This is an exciting opportunity to join a growing facility. kumc.wd5.myworkdayjobs.com/en-US/kumc-j...

Haha thank you Craig! Of course - you and all the others at PNCC have always been great help!