This protocol covers brain MRI and image segmentation for anatomical modeling and EEG signal reconstruction, validation of the oscillatory signals, oscillatory phase targeting accuracy and the main experiment of phase-coupled real-time EEG– TMS.
Readcube link:
Real-time EEG–TMS enables the timing of TMS pulses relative to target oscillatory brain states with millisecond-precision
@paolobelardinelli.bsky.social
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@natprot.nature.com
This workflow permits simultaneous profiling of chromatin accessibility and gene expression in single nuclei from tens to hundreds of samples and up to 1 million nuclei in a single run and is suitable for a wide variety of sample types.
Readcube link:
Single-cell ultra-high-throughput multiplexed sequencing leverages a combinatorial indexing strategy in which chromatin and mRNA are indexed in situ using sample-specific barcodes, and encapsulated into microfluidic droplets. @tommytang.bsky.social
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@natprot.nature.com
the hierarchical alignment of the nanofibers create pathways for the migrating cells, facilitating their rapid and large-scale transportation from the surrounding tissues to the wound.
Readcube link:
A protocol for 3D aligned nanofiber scaffolds provides spatial support for the repair of trauma-induced tissue defects
🧪 #materials
@natprot.nature.com
The method uses dual-scale spatial encoding through anchored rolling circle amplification for short-range proximities and hydrogel-constrained in vitro transcription for long-range associations, enabling volumetric reconstruction that overcomes previous two-dimensional limitations.
Readcube link:
Volumetric DNA microscopy encodes spatial information into DNA sequences rather than photons, enabling template-free reconstruction of 3D morphology and gene expression patterns in intact specimens through computational analysis of sequencing data. @jaweinst.bsky.social
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@natprot.nature.com
Acoustic ejection mass spectrometry cuts analysis times from minutes to a single second per sample by fragmenting the original sample and thereby avoiding complex workflows which would otherwise need to be customized for each one, separately.
Readcube link:
A protocol for determining chemical reactions using acoustic droplet ejection mass spectrometry directly from starting materials enables the fast analysis of hundreds of samples.
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@natprot.nature.com
The resulting COFs have large surface areas and high crystallinity, making them suitable for many applications, including photocatalysis, chemical sensing and gas sorption. The method has already been used to prepare 62 COFs with diverse linkages and topologies.
Readcube link:
Here, a protocol offering a probe-based sonochemical method for rapidly (under 1 h) synthesizing covalent organic frameworks (COFs) in an aqueous environment under ambient atmosphere, avoiding harmful solvents and high-pressure systems.
🧪 #materials
@natprot.nature.com
The procedure includes a workflow to generate a composite aging index (CAI) for microplastics that integrates weighted metrics from multiple analytical techniques.
Readcube link:
Microplastic aging is a dynamic process that depends on the chemical composition, size and shape of the material, and its environmental conditions. Here, a protocol to characterize aged microplastics in soil, water, air and organic material.
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@natprot.nature.com
The approach is designed to preserve the condensates in their native cellular state and captures stimulus-responsive condensate dynamic shifts. It provides proteome-wide coverage, enabling unbiased discovery of constitutive and inducible condensates.
Readcube link:
Here, a protocol offering a high-throughput strategy to identify endogenous biomolecular condensates and phase-separating proteins by integrating sucrose density gradient centrifugation with quantitative mass spectrometry.
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www.nature.com/articles/s41...
“I treated that access as something to be quiet about rather than discussed freely with others”
Peter Attia, explaining how to move in philanthropic circles and raise funds.
Desk reject.
🧪 #academicsky
Rooting for it! 🍀
CRISPRi-based circuits can integrate different signals to achieve programmable gene expression in plants, while the high-throughput protoplast-based assay permits rapid and scalable testing of circuit components across different species.
The procedure covers engineered CRISPRi genetic circuits in plants, and outlines the isolation of protoplasts from different plant species and tissues, and for testing genetic parts and gene circuits using a plate-based, high-throughput, dual luciferase protoplast transfection assay.
Where is the #Arabidopsis community on @bsky.app? Any #Brassica fans? See our recent protocol covering design principles and considerations for building and testing programmable and reversible CRISPRi-based gene circuits in plants.
🧪 #plantscience @natprot.nature.com @ryanlister.bsky.social
The procedure covers diverse experimental models to study neuron–GBM interactions, including the generation of GBO-cortical organoid assembloids, the transplantation of GBM cells into mouse brains and the co-cultures of GBOs with human primary brain tissue surgical slices.
This protocol describes rabies virus-mediated retrograde transsynaptic tracing and HSV-mediated anterograde transsynaptic tracing with GBM cells in xenografts and co-cultures, providing a platform for the systematic characterization of neuron–tumor connectomes.
A Protocol for characterizing the interactions between tumors and neurons in patient-derived GBM organoids:
🧪🧠 @natprot.nature.com
Montage parallel array cryo-electron tomography produces montages and individual tile tomograms, thereby eliminating possible mechanical adjustments to the transmission electron microscope.
Coupling 3D correlative focused ion beam milling with montage cryo-electron tomography enables the precise localization of regions of interest deep within cells and the acquisition of larger fields of view that preserve high-resolution structural details.
A workflow for 3D correlative cryo-fluorescence light microscopy-FIB-ET that streamlines fluorescence light microscopy-guided FIB milling, improving throughput while preserving both structural and contextual information
🧪🔬 #imaging @natprot.nature.com @erwright73.bsky.social
