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Nicolas Denans

@nicolasdenans

Scientist- Jack of all trades - Interest in #microscopy #immunology #devbio

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06.11.2023
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Latest posts by Nicolas Denans @nicolasdenans

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GitHub - saalfeldlab/lens-correct: Correcting lens-distortion in confocal stacks Correcting lens-distortion in confocal stacks. Contribute to saalfeldlab/lens-correct development by creating an account on GitHub.

If your imaging system has poorly characterized constant distortions leading to chromatic aberration, shoddy stitching, or false measurements (the answer is likely yes), you may want to give this tool a try. Native installers for Linux, Mac, Win under Releases github.com/saalfeldlab/...

20.02.2026 20:51 πŸ‘ 26 πŸ” 8 πŸ’¬ 0 πŸ“Œ 0
Conferences Application deadlines vary by meeting. See descriptions for more details.

So many great conferences @hhmijanelia.bsky.social . Don't miss the opportunity to participate in one of these unique experiences! www.janelia.org/you-janelia/...

18.02.2026 18:15 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

Are you working at the interface of #neuroscience and ML/AI? Share your research at Janelia in Nov! 🧠 πŸ€– janelia.news/NAI26

18.02.2026 17:23 πŸ‘ 12 πŸ” 5 πŸ’¬ 0 πŸ“Œ 0
Fluorescent Proteins and Biological Sensors VIII Organizers Eric Schreiter, HHMI/Janelia Research Campus Alison Tebo, HHMI/Janelia Research Campus Invited Participants Xiaolu Cambronne, University of Texas at Austin Katharine Diehl, University of

We’re excited to announce the next Fluorescent Proteins & Biological Sensors conference! Abstract submissions are now openβ€”hope to see you there.
www.janelia.org/you-janelia/...

17.02.2026 22:48 πŸ‘ 17 πŸ” 13 πŸ’¬ 0 πŸ“Œ 0
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πŸ§ͺ We are excited to share this novel open access paper on Phasor Mixing Coefficient to analyze colocalization, developed by folks @i2janelia.bsky.social and @aicjanelia.bsky.social. This is also the first technical paper jointly published with our sister imaging center @malacridalab.bsky.social!

28.01.2026 16:20 πŸ‘ 45 πŸ” 21 πŸ’¬ 2 πŸ“Œ 2

Wow, @erturklab.bsky.social team did it again! Bringing science fiction to the real world πŸ‘‹πŸ‘‹

18.12.2025 15:01 πŸ‘ 2 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0

really cool, congrats! #microscopy

16.12.2025 19:05 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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How do I get started with image analysis? - FocalPlane How do I get started with image analysis? - AIC at HHMI Janelia

Getting started with analysis can be daunting, especially given the many tools available. In our latest @focalplane.bsky.social post, @scientistrachel.bsky.social & Owen Puls discuss framing analysis questions to extract informative biological information. focalplane.biologists.com/2025/12/15/h...

15.12.2025 14:29 πŸ‘ 9 πŸ” 8 πŸ’¬ 0 πŸ“Œ 1

Pretty cool tool! Would have been extremely useful during my academic career. #Microscopy

08.12.2025 14:02 πŸ‘ 4 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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AIC at HHMI Janelia Introduction to the AIC at HHMI Janelia blog on FocalPlane

πŸ“’Did you know that researchers from around the world can apply for access to the latest microscopy technology & expert advice @aicjanelia.bsky.social?

In this blog series, the AIC team will give us an insight into how the Center works & their global impact.
focalplane.biologists.com/2025/11/20/a...

24.11.2025 13:42 πŸ‘ 7 πŸ” 4 πŸ’¬ 0 πŸ“Œ 0
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Chemical Tools for Complex Biological Systems IV Organizers Luke Lavis, HHMI/Janelia Research Campus Matthew Bogyo, Stanford University Zev Gartner, University of California, San Francisco Jennifer Prescher, University of California, Irvine

ahem, ONE of THE chemical biology conferences of 2026:) www.janelia.org/you-janelia/...

11.11.2025 18:03 πŸ‘ 16 πŸ” 7 πŸ’¬ 1 πŸ“Œ 1
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Another #FluorescenceFriday contribution for #Halloween a dive inside zebrafish vasculature

31.10.2025 20:37 πŸ‘ 10 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
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For #SpookyMicroscopyFriday I present the mugshot of the head of the zebrafish mafia

31.10.2025 12:49 πŸ‘ 8 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
A microscopy image of a cell stained for actin (magneta color) and focal adhesions (green color).

A microscopy image of a cell stained for actin (magneta color) and focal adhesions (green color).

Our team is hard at work preparing for #InsightFromImaging 2025! We can't wait to welcome HHMI #HannaGrayFellows to @hhmijanelia.bsky.social next week for lectures, hands-on labs, and activities focused on designing informative and quantitative bioimaging experiments πŸ”¬

27.10.2025 17:24 πŸ‘ 14 πŸ” 9 πŸ’¬ 0 πŸ“Œ 1

and the food...... It was raining like crazy the only time I went to the Monod conference but I still remember the food

23.10.2025 20:21 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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For #MicroscopyMonday a cleared mouse embryos labeled with an antibody targeting neurofilaments (2H3, DSHB) @hhmijanelia.bsky.social @aicjanelia.bsky.social

20.10.2025 17:19 πŸ‘ 3 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Mesoscale volumetric fluorescence imaging at nanoscale resolution by photochemical sectioning Optical nanoscopy of intact biological specimens has been transformed by recent advancements in hydrogel-based tissue clearing and expansion, enabling the imaging of cellular and subcellular structure...

for #FluorescenceFriday : is your ExM sample too large to be imaged with high NA objectives? VIPS it! Very clever way to tackle one of the biggest challenge in large volumetric ExM www.science.org/doi/10.1126/...

17.10.2025 12:29 πŸ‘ 20 πŸ” 6 πŸ’¬ 0 πŸ“Œ 1

very cool! What imaging modality did you use to spy on these cells?

14.10.2025 12:05 πŸ‘ 1 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0

the scalewiew-A2 (25x) .95NA 8mmWD is probably your best bet right now. Between 2-3mmWD I go with Nikon for >1NA

28.09.2025 15:11 πŸ‘ 2 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0
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The WB-ExM protocol described here works with.... every sample we tested! Here a 3do quail embryo (white= pan-protein; red=MF20) www.biorxiv.org/content/10.1...

19.09.2025 19:39 πŸ‘ 6 πŸ” 3 πŸ’¬ 0 πŸ“Œ 0
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Today was a super fun first day of imaging @aicjanelia.bsky.social with @vanderspuy.bsky.social and @samarar.bsky.social.
All hail the quail!οΏΌοΏΌπŸ§ͺπŸ”¬#DevBio

21.07.2025 23:44 πŸ‘ 15 πŸ” 4 πŸ’¬ 0 πŸ“Œ 0
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Research Specialist - Tavakoli Lab Primary Work Address: 19700 Helix Drive, Ashburn, VA, 20147 Current HHMI Employees, click here to apply via your Workday account. The Howard Hughes Medical Institute’s Janelia Research Campus is a pio...

🧠 Excited by synapse-level brain mapping? Join us at a world-class institute to build molecular tools based on our new #LICONN work β€” and push the frontier of #MolecularConnectomics. Let’s decode the brain, one synapse at a time.

hhmi.wd1.myworkdayjobs.com/en-US/Extern...

17.07.2025 14:56 πŸ‘ 13 πŸ” 8 πŸ’¬ 0 πŸ“Œ 3

Just over a week left to apply for this round of the AIC Call for Proposals! Submit at www.aicjanelia.org/apply by Monday, June 16 (5 pm US ET).

06.06.2025 16:12 πŸ‘ 6 πŸ” 7 πŸ’¬ 0 πŸ“Œ 2
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GEF25-Homepage - rizzoli-lab Program Location & Travel About Home GEF25, organized in the 10th anniversary year of ExM, focuses on cutting-edge advancements in expansion microscopy, as a part of global initiatives to democratize ...

Is this amazing ExM conference still happening? www.rizzoli-lab.de/gef25/

13.05.2025 16:30 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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New technique expands tissues so hundreds of biomolecules can be seen inside cells A new tissue expansion method developed by Janelia and University of Wisconsin-Madison researchers enables scientists to use mass spectrometry imaging to simultaneously detect hundreds of molecules

πŸ₯ΌA new tissue expansion method developed by researchers in the Wang & Tillberg labs & collaborators @uwmadison.bsky.social enables scientists to use mass spectrometry imaging to simultaneously detect hundreds of molecules at the single cell level in their native locations ➑️ hhmi.news/4ixHOZZ

22.04.2025 14:45 πŸ‘ 29 πŸ” 12 πŸ’¬ 0 πŸ“Œ 0
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A highly stable monomeric red fluorescent protein for advanced microscopy - Nature Methods The red fluorescent protein mScarlet3-H is bright, photostable and very robust to high temperature, chaotropic conditions and oxidative environments. mScarlet3-H works well in correlative light and el...

I guess they renamed their protein from mYongHong to mScarlet3H www.nature.com/articles/s41...

17.04.2025 13:17 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

A new call for proposals just opened with @aicjanelia.bsky.social . Do not miss this unique opportunity to collaborate with microscopy experts to advance your project!

15.04.2025 13:25 πŸ‘ 2 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0

Amazing opportunity!! #microscopy #bioImaging

12.02.2025 17:22 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

Looks impressive! Check it out!

11.02.2025 19:30 πŸ‘ 2 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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BioImage Analyst Primary Work Address: 19700 Helix Drive, Ashburn, VA, 20147 Current HHMI Employees, click here to apply via your Workday account. The Howard Hughes Medical Institute’s Janelia Research Campus is a pio...

Do you want to help developing new groundbreaking methodologies with US @i2janelia.bsky.social ? We need another BioImage Analyst to join our growing team! hhmi.wd1.myworkdayjobs.com/en-US/Extern...

11.02.2025 14:41 πŸ‘ 2 πŸ” 2 πŸ’¬ 0 πŸ“Œ 0