Albert Heck

These HTA proteases from archaea work great for mass spec-based antibody sequencing. Paper out now in final form at Cell Systems. Congrats to @tshamorkina.bsky.social @tkadava.bsky.social @lperezpaneda.bsky.social and @hecklab.bsky.social
www.sciencedirect.com/science/arti...

Original (left) and corrected (right) Fig. 4a in the paper https://www.nature.com/articles/s41586-026-10305-0 showing the chemical structures of amino acids (only the right one) the left figure is just an embarrassment

I wonder... 🤔🤔🧪 #chemsky

a) what program did the authors use to make the original (left) figure in the paper? Wild guess, ChatGPT

b) how such high school science level of disgrace pass peer review in @nature.com?

Note this was Fig.4.a NOT in SI

www.nature.com/articles/s41...

Got an Orbitrap-based mass spec that couldn’t do STORI/direct mass technology before (any aside from the UHMR and Tribrid Ascend)?

This is a cool open-source implementation that even works on an Orbitrap Elite! #teammassspec

Is it not the more complicated form of mass spectroscopy?

A Universal Spectrum Annotator for Complex Peptidoforms in Mass Spectrometry-Based Proteomics Accurate and comprehensive peptide spectrum annotation is a crucial step to interpreting mass spectrometry-based proteomics data. While peak assignment in peptide fragmentation spectra is central to a...

A Universal Spectrum Annotator for Complex Peptidoforms in Mass Spectrometry-Based Proteomics | Analytical Chemistry pubs.acs.org/doi/10.1021/.... Making your data better viuable, no matter whether it is "boring" proteomics, or more exciting glycoproteomics, cross-linking mass spectrometry etc.

Interested in ultra low input phosphoproteomics? Here we summarised the current considerations and opportunities when working with rare populations of cells. Congrats to @emgaiz.bsky.social! #TeamMassSpec

#HUPO2025 interested in #topdownproteomics please come to join us in the Friends of HUPO reception 18:45-19:45 pm Westin Frontenac Ballroom Foyer 😀❄️ @nlkproteomics.bsky.social @proteoforms.bsky.social @jyates.bsky.social @hecklab.bsky.social #teammassspec

Every day’s commute on bike. Not every day is the same

Since early 2024 we have been working with @brukercorporation.bsky.social on the first prototype timsOmni instrument, integrating an Omnitrap into a timsTOF. Here we report for the first time on the use of this instrument for protein-centric antibody sequencing. See www.biorxiv.org/content/10.1...

🔬 For more, delve into this paper published in Molecular & Cellular Proteomics ( @asbmbjournals.bsky.social): www.mcponline.org/article/S153....

Congratulations to @tkadava.bsky.social, @hecklab.bsky.social and colleagues on this fascinating work!

It was such a pleasure to participate in such a diverse symposium with high quality contributions. And ended perfect with joint bike ride around Freising. Thank you @baybioms.bsky.social

Thermal Tracks: A Gaussian process-based framework for universal melting curve analysis enabling unconstrained hit identification in thermal proteome profiling experiments Thermal Tracks is a Python-based statistical framework for analyzing protein thermal stability data that overcomes key limitations of existing thermal proteome profiling (TPP) work-flows. Unlike stand...

For those who perform thermal proteome profiling (TPP) experiments, you might find this new data analysis approach (and open source Python software) useful. Developed by postdoc @jfhevler.bsky.social 🙏

arxiv.org/abs/2508.096...

🧵(4/7)

🔬 We have put together a fascinating #Keynote #Speakers team:
• Prof. Albert Heck - @hecklab.bsky.social
• Dr. Hamish Stewart - @hamishs.bsky.social
• Prof. Olga Vitek - @olgavitek.bsky.social l
• Prof. Ron M.A. Heeren

My lab @stanfordmedicine.bsky.social is recruiting! We are looking for a postdoc at the interface of quantitative proteomics and G protein-coupled receptor (GPCR) biology:
postdocs.stanford.edu/prospective/...
#TeamMassSpec #Proteomics #GPCR #Postdoc

Having a great time at msandm2025.org with a great bunch of people interesting in visualizing biomolecules, together with my co-chairs @joostsnijder.bsky.social @vixklen.bsky.social & Kyle Fort. Learning so much from all posters & lectures. We thanks all participants for joining us in Utrecht.

We’re hosting the MS+M conference in Utrecht today, lots of great talks ahead at the intersection of mass spec and microscopy. Here’s @hecklab.bsky.social kicking things off.

Breaking barriers in the sensitive and accurate mass determination of large DNA plasmids by mass photometry Heck and colleagues developed a mass photometry-based method for accurate and fast mass determination of plasmid DNA. Conventional mass photometry methods are not applicable for large DNA particles; h...

Breaking barriers in the sensitive and accurate mass determination of large DNA plasmids by mass photometry: Molecular Therapy Nucleic Acids www.cell.com/molecular-th.... Even visualizing > 10 million Da plasmids by the incredible Eduard Ebberink

Preparations in full swing. Exciting program, looking forward to msandm2025.org and welcome our guests in Utrecht to discuss mass spectrometry, atomic force & electron microscopy and single molecule techniques

Structural Insights Into Complement Inhibition: Visualizing Distinct Binding Modes of C4b-Binding Protein Complexes With C4b and SAP - Molecular & Cellular Proteomics www.mcponline.org/article/S153.... See this work of @tkadava.bsky.social @utrechtuniversity.bsky.social artistically interpreted

Great line-up

🕷️Spiders in your blood? Don’t worry, these are protein molecules of C4BP protein, part of your immune system. Just like real spiders, they grab prey with their “legs.” Watch them in action! Intrigued, check the paper of @tkadava.bsky.social doi.org/10.1016/j.mc... @bijvoet-centre.bsky.social

Slice-PASEF: Maximising Ion Utilisation in LC-MS Proteomics Quantitative mass spectrometry (MS)-based proteomics has become a streamlined technology with a wide range of usage. Many emerging applications, such as single-cell proteomics, spatial proteomics of t...

www.biorxiv.org/content/10.1...

Our graduate student, Holden Rogers and Marie Curie postdoc fellow, Boris Krichel, did a fantastic job presenting their research projects at #TDP2025👍👏😀

#TDP2025 @proteoforms.bsky.social a great opening session with fantastic talks! @nlkproteomics.bsky.social @joelooucla.bsky.social @hecklab.bsky.social @metamorpheus.bsky.social 👍thanks Hartmut for organizing the conference! 👏

🥁🥁🥁
The 51st Lorne Conference on Protein Structure and Function is officially live! After the resounding success of the 50th Lorne Conference in 2025 celebrating five decades of pioneering science and community, the legacy continues... 🏄🏖️🌞

February 8–12, 2026

Register here: www.lorneproteins.org

Nice interview with star PhD Shelley!

Revolutionising plasma glycoproteomics with Orbitrap Astral and narrow-window DIA - IonOpticks Jager et al.'s study into plasma glycoproteomics uses a 25 cm x 75 μm IonOpticks Aurora Ultimate 25x75 UHPLC column.

New plasma glycoproteomics method out of the @hecklab.bsky.social uses narrow-window DIA on Orbitrap Astral with Aurora Ultimate XT column. 50% more glycopeptide IDs vs. traditional approaches, with 181 glycoproteins detected across 7 orders of magnitude in just 10-40 minute runs.

bit.ly/45bDXhv

Wish I could!

Structural Insights into Complement Inhibition: Visualizing Distinct Binding Modes of C4b-binding Protein Complexes with C4b and SAP C4b-binding protein (C4BP) is an innate immune inhibitor found in serum. Human C4BP adopts spider-like higher-order structures (HOS) formed by disulfide-linked C4BPα and C4BPβ chains that non-covalent...

🕷️ Spiders from our blood 🕷️
Check out our new paper from @hecklab.bsky.social describing how the spider-like immune regulator C4BP interacts with its native binding partners.
🔗 www.mcponline.org/article/S153...
#MassSpec #StructuralBiology

We show that C4BP has two distinct binding modes, either involving a single “spider leg” or the entire spider-like structure wrapping around the target.
Thanks to everyone involved!
@victoryin.bsky.social, @hecklab.bsky.social, Jürgen, Johannes, Maryam, and Leendert