Benjamin B Johnson

Sure thing, you’re added!

Already have you in Cathy 😉

You’re in my friend 👍🏻

Sure, you’re in! 👍🏻

Absolutely, you’re in! 👍🏻

Cardiac scratch wound assay to show the role of ECM in wound healing. HiPSC Cardiomyocytes in white on the boarders with a rich ECM scar tissues (cyan) through the middle with a simple nuclear stain (red) highlighting all cells.

How to fix a broken #heart 💔->❤️

#Proteoglycan rich #ECMatrix scar tissue is laid by migratory cells after a cardiac scratch wound with #hiPSC #Cardiomyocytes.

Happy #Fluorescencefriday #BlueSky & #CardioSky!

@ablanchasensio.bsky.social 👀
#Science 💥🧪 #Microscopy 🔬

DNA during cell death and cell division.
#Science #Biology #CellBiology #microscopy

Sure, done!

👍🏻

Sure thing, you’re in!

Added! 👍🏻🧪

Sure, done! 👍🏻

Hi James, would love to be added thanks!

Genetically-modified hiPSCs expressing the genetically-encoded calcium indicator jRCaMP1b with a nuclear export signal (NES). This reporter is used to track calcium dynamics in live cells. It combines a red fluorescent protein with calmodulin and a calcium-binding domain, changing fluorescence intensity upon calcium binding. Its red-shifted emission is ideal for imaging in complex tissues or dual-color experiments, making it valuable for studying calcium-mediated processes. A single copy of this reporter was integrated using the STRAIGHT-IN platform at the AAVS1 locus. See below! https://doi.org/10.1016/j.crmeth.2022.100300 https://doi.org/10.1101/2024.10.17.616637

Here is my 2nd #FluorescenceFriday post! 🧪 #hiPSCs expressing jRCaMP1b, a genetically-encoded Ca2+ indicator 👻. This reporter allows us to record intracellular Ca2+ in hiPSC-derived #cardiomyocytes and cardioids (videos in the comments) #microscopy 🔬 #Science #Biology @benjaminbjohnson.bsky.social

There are so many #morphogenesis researchers here!
My first starter pack is full!
Here is Part 2: go.bsky.app/K6YYKeC

#devbio #cellbio #science

Tell me if I should add you but try to make sure, that you're not already in Part 1.
I cannot cross-reference everyone 😅

Part 1: go.bsky.app/RzDD9or

Hi, could I be added to your pack thanks!

Hey Jeff, would love to be added to your pack thanks!

Hey, would love to be added! I work on hPSC cardiac systems and disease models ❤️

Hey, would love to be added thanks! 😀

Hey, would love to be added to your pack thanks! ❤️

Hey, can I be added please and thanks! 😀

Hey! Would love to be added to this pack, thanks! ❤️

Cyan cellular extracellular matrix fibers are highlighted laying over the top of the orange hyaluronan secreted matrix after a scratch would assay.

Dedicating this #Fluorescencefriday to the #ECMatrix lovers. Was trialing some ECM staining methods on a scratch wound and love the HABP staining looking almost like flames 🔥🧪🔬

Lets see what you got, @ablanchasensio.bsky.social 😉

Definitely, you’re in!

Absolutely, you’re in! 👍🏻

Done!

Created a starter pack focusing on disease modeling in a dish using stem cells! 🧪🔬

Please (self)nominate anyone you think should be added to the list and repost so it finds the right people ❤️

hPSC Disease Modeling: go.bsky.app/EpWsxNG

For a good general stem cell pack go here: go.bsky.app/UTW4d7b

Graphical abstract depicts automated setup to generate 3D cardiac mini tissues consisting of 3 cell types seeded into 384 well plates. The cardiac tissues are monitored using calcium traces to detect arrhythmias and subsequent rescue after compound addition.

🔥Super excited to drop our recent preprint🔥

biorxiv.org/cgi/content/...

Here, we automated the generation of 3D Cardiac Microtissues and performed a small high-throughput screen to identify several novel anti-arrhythmic compounds for CPVT1 🧪❤️

#Cardiosky #Science #hiPSC #Arrhythmia #Stemcell

Science! 🧪

Hey #BlueSky! Here is my first #FluorescenceFriday post!
This cardiac organoid was derived from #hiPSC and imaged using light sheet #microscopy 🔬.

🔵 Cardiomyocytes
🔴 Endothelial cells
🟡 Fibroblasts

More coming next week! 🧪

#Science #Biology #CellBiology #SciArt
@benjaminbjohnson.bsky.social