Mike Neugent PhD

Overview of the paper in cartoon form

We are delighted to share our vision in #RSCChemBioy of how to incorporate natural product discovery (and any drug discovery) into the framework of personalized medicine - drug discovery using patients, but not in patients! #natprod #secmet pubs.rsc.org/en/content/a...

Did it work?
🤷🏽

But its pretty

Thank @svetlanaup.bsky.social ! I am so excited to get started!

I’m thrilled to announce that the Neugent Lab will officially set sail on May 1st, 2026 in the Department of Urology at the University of Rochester Medical Center!

My lab will investigate how we can manipulate the ecosystem of host / #microbiome interfaces to impact urologic health.

Thanks Ramon! I hope you are doing well!

I'm honored to join the URMC Urology team and can't wait to get to work.

Stay tuned for updates on recruitment opportunities as we build our team of scientists!

#Urology #Microbiome #CancerResearch #InfectiousDisease #NewPI #WomensHealth

I’m thrilled to announce that the Neugent Lab will officially set sail on May 1st, 2026 in the Department of Urology at the University of Rochester Medical Center!

My lab will investigate how we can manipulate the ecosystem of host / #microbiome interfaces to impact urologic health.

Sodium ion-assisted structural lipidomics for sphingolipid profiling Sphingolipids are diverse lipids with sphingobases and N-acyl fatty acids as the hydrophobic moieties. While the importance of the in-depth elucidation of hydrophobic structures is widely recognized in lipid biology, mass spectrometry-based annotation of ceramides in the commonly used protonated form is often hindered by in-source dehydration during electrospray ionization in the heated state and variable water losses in the product ion spectrum. In this study, we investigated the sodium ion form and its product ions in ceramides with the use of electron-activated dissociation tandem mass spectrometry (EAD MS/MS) in addition to collision-induced dissociation to facilitate in-depth structural elucidation. While dehydrated ions from the protonated form were frequently observed, the sodium adduct ions remained stable because of their higher activation energy compared with the protonated form, which was validated using quantum chemical calculations. Using the three adduct forms under optimized conditions increased confidence in annotating the ceramide peaks through retention-time matching. Furthermore, EAD MS/MS of the sodium adduct ions facilitated the positional determination of double bonds and hydroxyl groups in the ceramide hydrophobic moiety. Our approach is showcased by the annotation of phytoceramides with N-acyl 2- and 3-hydroxyl groups in mouse feces and ceramides with N-acyl n-6 very long-chain polyunsaturated 2-hydroxy fatty acids in mouse testis.

(BioRxiv All) Sodium ion-assisted structural lipidomics for sphingolipid profiling: Sphingolipids are diverse lipids with sphingobases and N-acyl fatty acids as the hydrophobic moieties. While the importance of the in-depth elucidation of hydrophobic structures is widely… #BioRxiv #MassSpecRSS

Fungal infection drives metabolic reprogramming in epithelial cells via aerobic glycolysis and an alternative TCA cycle shunt Candida hijacks epithelial metabolism via glycolysis and GOT1-dependent TCA shunt, influencing antifungal defense.

If you’re interested in host–microbe interactions, immunometabolism, mucosal biology, or fungal pathogenesis, I hope you’ll check out the full article. 👇

@science.org

www.science.org/doi/10.1126/...

Happy new year!! I cant believe it’s already 2 weeks into 2026… going too fast!

Here’s my ugly coffee this morning… decaffeinated… not worthy of a pretty pic :)

Join #MVIF 44 cassyni.com/s/mvif-44
and #MeetTheSpeakers

🇺🇸 Vanessa Hale will discuss technical challenges of low microbial biomass and high host cell shedding in metagenomics analysis of urine samples.

Check out the paper: microbiomejournal.biomedcentral.com/articles/10....

Nanoscale imaging of native symbiotic animal tissue using amultimodal large volume imaging pipeline for cryo-electrontomography https://www.biorxiv.org/content/10.1101/2025.11.30.691379v1

Urinary biochemical ecology reveals microbiome-metabolite interactions and metabolic markers of recurrent urinary tract infection - npj Biofilms and Microbiomes npj Biofilms and Microbiomes - Urinary biochemical ecology reveals microbiome-metabolite interactions and metabolic markers of recurrent urinary tract infection

Thrilled to share that our recent work is out in npj Biofilms and Microbiomes. 🧵 (1/4)

www.nature.com/articles/s41...

Further announcements coming soon on the future of this work as we continue clinical translation and look toward a more personalized management of rUTI.

Our goal: understand how microbial communities and metabolites shape the postmenopausal urobiome and susceptibility to recurrent UTI (rUTI). We further uncover joint biochemical/microbial markers that could improve diagnosis, prognosis, and clinical management.

In this work, we mapped urinary biochemical ecology to urobiome microbial ecology by pairing quantitative metabolomics with shotgun metagenomics in a cohort of postmenopausal women designed to study recurrent UTI.

Urinary biochemical ecology reveals microbiome-metabolite interactions and metabolic markers of recurrent urinary tract infection - npj Biofilms and Microbiomes npj Biofilms and Microbiomes - Urinary biochemical ecology reveals microbiome-metabolite interactions and metabolic markers of recurrent urinary tract infection

Thrilled to share that our recent work is out in npj Biofilms and Microbiomes. 🧵 (1/4)

www.nature.com/articles/s41...

Hey!!! Ok i downloaded the bsky app so i can send you some coffee cheers too!

Just 3 months late! Cheers!

Bladder Wall–Embedded Bacteria Associated With Increased... : JU Open Plus eria within the bladder walls of women with rUTI. We hypothesized that increased bladder wall bacterial burden may be associated with incomplete rUTI resolution after EF. Materials and Methods: Aft...

My first author paper is out on JU Open Plus.Check it out!

Bladder Wall–Embedded Bacteria Associated With Increased... : JU Open Plus journals.lww.com/juop/fulltex...

That was so much fun!!! Love giving these workshops!

Zero to hero in statistical analysis in R!

This feels like a critical next step for microbial ecology. 🌍
Not just “who’s there,” but what kind of world they’re living in.

Let’s talk. Who’s working on this?
👇👇👇

#microbiome #metabolomics #molecularnetworking #systemsbiology #datascience #ecology

Then take it a step further:
Can we jointly assess microbial and chemical beta diversity in a single, unified analysis? 🔗🧫📊
Imagine the insight we could gain.

Can we build something like UniFrac, but for chemicals? 📐💥
We already have MS2 spectral similarity through tools like GNPS molecular networking—why not use this to define biochemical beta diversity?

Microbes live in biochemical environments they help shape—this is the biochemical ecology. 🧬🌱
Untargeted metabolomics captures this, but we still lack great tools to compare samples in ecological terms.

It’s like counting animals on a savannah without noticing the grass, trees, rocks, or soil. 🐘🌾🌳🪨
You get a species list—but not the ecosystem that gives it meaning.

What are we not seeing in the microbiome? 🤔
Metagenomics tells us who’s there—but not what surrounds them.
That’s a huge blind spot in microbial ecology. Let me explain. 🧵

Urinary biochemical ecology reveals microbiome-metabolite interactions and metabolic markers of recurrent urinary tract infection Recurrent urinary tract infections (rUTIs) are a major clinical challenge and their increasing prevalence underscores the need to define host-microbiome interactions underlying susceptibility. How the...

🚨 Preprint update

Excited to share our updated preprint on the biochemical ecology of recurrent UTI.

🧬 #Metagenomics + #metabolomics
🧪 Lipid biomarkers for active UTI (AUC = 0.91)
⚠️ DCA as a predictor of recurrence

🔗 doi.org/10.1101/2024...

#UTI #Microbiome #Metabolomics #PrecisionMedicine

TODAY

Are you interested in learning more about the urobiome?

Join the CAIRIBU U-RIG Research Hour to hear talks from Seth Reasoner, titled "Pathogen Evolution in Neurogenic Bladder" and Katy Patras, titled "The Vaginal Microbiota and Colonization by Uropathogens."

NEXT MONTH

Join Urobiome 2025 on February 24th, 2025 in San Diego, CA. Learn more about the state of urobiome research from expert investigators!

More information HERE: https://buff.ly/3PHUl0W