Poly-ubiquitylated proteins proved trickier to purify for LC-MS than expected. Non-specific background swamped isolations: with histones and uncertainty!
This led us to develop Ubi-SCAPEβfor characterising the poly-ubiquitylome to unprecedented purity and depth.
www.biorxiv.org/content/10.6...
16.02.2026 15:56
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Pleased to use my first π¦ post to thank @mtrost.bsky.social and @drandyfrey.bsky.social for hosting a BBSRC CONNECT FTMA placement sharing proteomics & MS knowledge between @babrahaminst.bsky.social & @newcastleuni.bsky.social
Been here <20h and already got some exciting ubiquitin proteomics data!
18.11.2024 12:57
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