Since submission, we have a new version of the program with some improvements, and we have many developments in the pipeline for faster, more sensitive template matching. Keep an eye on our GitHub for these.
github.com/Lucaslab-Ber...
Now out in full form. Again, a big thanks to #teamtomo and @alisterburt.bsky.social for developing the infrastructure to build this on. journals.iucr.org/d/issues/202...
Chris Russo and Anastasiia Gusach have developed a new method to prepare #cryoEM samples that avoids protein damage during freezing
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
We are happy to announce that we will have two new scientific groups led by Andrija Sente and Katerina Naydenova soon! If you are interested to join their lab as a PhD Student apply at our @imprs-lm.bsky.social
www.imprs-lm.mpg.de/index.php/jo...
Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching www.biorxiv.org/content/10.1101/2025.09.11.675606v1 #cryoEM
Happy for this to be out! We have documentation and tutorials here. Please give it a try and let us know what you think. lucaslab-berkeley.github.io/Leopard-EM/
Pulsed-electron illumination does not reduce beam damage for imaging biological macromolecules www.biorxiv.org/content/10.1101/2025.07.29.667395v1 #cryoEM
@joshdcryoem.bsky.social will also be giving a talk about his prior work as a PhD student with Chris Russo developing strategies to take advantage of the reduced radiation damage at liquid Helium temperatures for cryo-EM. You can find their paper here: www.pnas.org/doi/abs/10.1...
If you are going to M&M, also see postdoc @joshdcryoem.bsky.social's poster about tilted vs untilted data collection for in situ structural biology www.sciencedirect.com/science/arti....
Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...
At the 2024 IMC meeting, Richard Henderson laid down a challenge. Design a #TEM single-particle #cryoEM lens with 1mm Cc at 100kV. I'm thrilled to reveal our new Arxiv preprint delivering a fully retrofittable pole-piece swap out for the @jeoleurope.bsky.social 1400 TEM.
arxiv.org/abs/2505.11458
In the Bay Area? Love computational cryo-EM/ET?
Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community!
Details and registration at frostbyte-cryoem.github.io
Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!
For a shorter read, @mrclmb.bsky.social and @science.org also have articles about the helium story. www.science.org/content/arti...
After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it.
🔗 www.pnas.org/doi/10.1073/...
Exploring shaped focused ion beams for lamella preparation https://www.biorxiv.org/content/10.1101/2025.04.01.646235v1
In-Situ High-Resolution Cryo-EM Reconstructions from CEMOVIS https://www.biorxiv.org/content/10.1101/2025.03.29.646093v1
Foam film vitrification for cryo-EM https://www.biorxiv.org/content/10.1101/2025.03.10.642366v1
I've been wanting to see a paper using off-axis electron holography for low-dose biological imaging since the start of my PhD. Spatially resolved charge measurements👌
Kexin Zhang et al.: A new statistical metric for robust target detection in cryo-EM using 2D template matching #3DReconstructionAndImageProcessing #SingleParticleCryoEM #Imaging @umassboston...#IUCr https://journals.iucr.org/paper?S2052252524011771
Laser Flash Melting Cryo-EM Samples to Overcome Preferred Orientation biorxiv.org/cgi/content/short/2024.11.21.624652v1
It should be relatively simple with the Bio.PDB package in Biopython. It has both pdb and mmtf IO modules.
