Congratulations to the Cater Lab's first ever graduate Parteek! Bachelor of Advanced Scince with First Class Honours - we are SO proud of you! Looking forward to another year of fun in the lab together in 2026! 🎓🔬🧪✨
ProteinDJ logo
I am thrilled to release ProteinDJ: a high-performance and modular protein design pipeline. Our open-source workflow incorporates #RFdiffusion, #ProteinMPNN, #FAMPNN, #AlphaFold2 and #Boltz-2. It is a fast, free, and fun way to design proteins (1/5)
doi.org/10.1101/2025.09.24.678028 #proteindesign
Thank you to all of our plenary speakers for sharing your incredible research with us at #ASBMB2025! @nanigrotjahn.bsky.social @ajknights7.bsky.social Michelle Haber and Randal Halfmann. We have loved having you all in Brisbane and hearing about your labs awesome work! 🦠🧬🧪🧫🔬
And that's a wrap! A huge shout out goes to our conference chair Michael Landsberg @dr-berger.bsky.social and the rest of the organizing committee for putting together such a vibrant #ASBMB2025! See you all at #ComBio2026!
Lorne 2026 is set to be excellent! Especially with a Transporter Satellite Meeting!!
👕✨ Design the next Lorne Protein Conference T-shirt!
Entries close 17 Oct – don’t miss your chance to show off your creativity and cement your place in the history of Lorne Proteins 🤓
👉 Submit your design-see the link below...
📢 And don’t forget to submit your abstract by 24 Oct!
#LorneProteins
DEADLINE extended till tomorrow!!! Decorate the ASBMB gelato cart with your cool microscopy image/favourite protein structure!!
Still time to sneak in an abstract for ASBMB! www.asbmb2025.com.au/2025-call-fo...
Just over 1 week left for ASBMB 2025 abstract submission!!!
Make sure you don't miss the deadline! WE want to hear the cool science YOU have to share!
www.asbmb2025.com.au
Add it to your calendars! Register and submit your abstract for ASBMB this month! 🗓️📃🧬🧫🔬
The ASBMB 2025 Conference is coming to UQ Brisbane – and the official website is now LIVE!
Explore the program, register early & submit your abstract now!
www.asbmb2025.com.au
#ASBMB2025 #UQ #biochemistry #molbio #academiclife #conferences
Why are biological membranes asymmetric, with different lipids in the two bilayer leaflets? Discovered in the 70s, lipid asymmetry is linked to many cellular processes, but why the cell needs it is largely unclear. @pavelbarahtjan.bsky.social addresses this question: www.biorxiv.org/content/10.1...
One more sleep to the @biophysicalsoc.bsky.social CRT Symposium on tomorrow morning bright and early! Join us in Petree Hall C at 8.30am.
Our inspirational Leach Lecturer Frances Separovic. A champion of women in STEM. "A reluctant chemist". Croatia to Broken Hill. A peace lover. First in family to finish primary school. And one cool Professor 😎. #LorneProteins2025
50 years of Lorne Proteins on one stage! Happy anniversary to us 😁 and the supportive and inclusive research culture we've built 🫶 #LorneProteins2025
How does it enhance phage defence? Pfu/mL +/- Csx28. 10000x improvement in phage defence by small membrane protein. Bacterial growth with addition of phage. Bacteriostatic effect? Forms an octamerif forward in DDM, now nanodisc... ligand gating of channel? Depolarisation assay? Dye that is weakly fluorescent when cells are polarised, loss of polarisation increase fluor. DiBAC4. 40% depolarised with cas13b and csx28. No complex formation. Cleaved ssRNA to open this channel. tRNA anticodon loop cleavage by target-activated CRISPR-Cas13a. Cleavage products from tRNAs. Pull down monomeric Csx28 no pulldown; Octomer- enrichment of ssRNA in pull-down. Pull downs on grids - 8 mol of tRNA degradation products bound to Csx28. Where is the tRNA cleaved? cmo5U? Xm5U? Keio collection - screened against tRNA modification enzyme kos. Modification of the wobble residue prevents the phage defence. Csx27 4 TM protein next. Similar function to Csx28. Alphafold2 to identify similar RNA gated ion channels.
Session 9: Mitch O'Connell bacterial/bacteriophage arms race. Small RNAs to mount an adaptive MGE immune response. CRISPR RNA defence systems. How does Cas13 work as an enzyme to target RNA? Csx28 128 aa protein, TM, cyt. Co-opt membrane effectors? 👏 #LorneProteins2025
High resolution in fixed cells, but temporal events - transient, sub diffraction clusters in live mammalian cells. -ve feedback - 4-5 polymerase load, clusters are released. Cells can hold clusters longer to increase transcriptional events. Dual colour super resolution. TcPALM. Measure active burst, estimate RNAs and correlate with cluster lifetime. Enhancer, mediator, PolII - Mediator and PolII in stem cells. Short lived 11 s. Subset large clusters 300-400 nm in size, long lived 10s of minutes. Lattice lightsheet. 3d, time lapse, 10s of minutes, a full nucleus every 15s or so. Some clusters fuse to merge content. In vivo condensates with liquid like properties. Condensate proximity to Sox2 promoter correlates to mRNA burst intensity. Du et al, Cell, 2024. Higher burst size, higher frequency of burst events. 1.5-2 fold higher proximal to a PolII condensate. Anticorrelation between distance and burst size. When condensate contacts enhancer and generates. If superenhancer is distal from condensate, then no burst. Mediator/PolII, Superenhancer DNA, nascent mRNA in 3 colours. Movie of condensate moving in to contact the superenhancer, Gene promoter active and mRNA increases. Is the condensate/superenhancer contact active? Yes, cohesion required to loop DNA - precise architecture of the DNA to achieve a burst. Cohesion loops DNA and stops at an enhancer element upstream of the promoter. Lee et al 2024, Condensates - complex, liquid droplets? Proximity mini turbo ID? Accumulate any cargo of interest to be concentrated in the condensate. Turn on blue light and generate condensate localisation? Light induced targeting of endogenous condensates. Cargo-miniturbo-ID-GFP blue light on, accumulates in condensate, biotinylation accumulating. LiTEC with BioID for proteomics on condensates. Some missing... Chimeric mining. Pass droplets through mass spec - often ignore low intensity peaks. Looked for background peaks. More to follow up..
Session 10 Ibrahim Cissé-Super-res imaging of transcription. Cooperativity in weak & transient biological interactions. PolII clustering in a cell nucleus w STORM. Limitations for weak and transient interactions. Lightsheet microscopy to follow polymerase condensates & gene bursts #LorneProteins2025
Excellent talk, impressive techniques, definitely going to look into biomolecular condensates in prokaryotes
Exciting new position at UC Berkeley! Cal-Cryo is looking for a new scientific director! This is an ideal position for someone who is looking to continue doing science and collaborating with research teams at Berkeley. Come work with us! aprecruit.berkeley.edu/JPF04670
As Lorne Protein Meeting approaches its 50th anniversary we’re excited to welcome the fantastic speakers coming in 2025 to celebrate.
www.lorneproteins.org/speakers2025
Ok, trying to bring my community back together... I created a starter pack for lipids and membrane protein aficionados!
Feel free to share, add and distribute, so we can come together again! 😍
go.bsky.app/AEeXh86
This week in Science Without Anguish: Who is driving our bus? The importance of controlling the controllables in research life.
www.sciencewithoutanguish.com/blog/who-is-...
🚨 #DiscoveryProjects #DP25 announcement:
❗️Outcomes announced publicly for Discovery Projects 2025❗️
See ARC's RMS for list ➡️ rms.arc.gov.au/RMS/Report/D...
/bot
Screenshot of a tweet from the ARC saying they’ll announce DP25 grant outcomes tomorrow (Tuesday 26 Nov).
ARC says they’ll release the Discovery Projects 2025 outcomes tomorrow.
Recently, outcomes announcements have tended to happen at/around 11am Canberra time.
My bot will pick up the announcement in RMS’s database and post immediately.
Good luck!
This week we had the pleasure of hosting cryo-EM guru @OliBClarke who gave the @UQ_News cryo-EM community a fantastic cryo-EM data-processing workshop. Thank you Oli for sharing your tricks with us, @FarrahBlades for wrangling the Bunya supercomputer, and all who attended! 🔬
Want to Structure, Function, Surf, Repeat?!
@LorneProteins is renowned for being an outstanding international protein conference in a beautiful Aussie beach town. Have a read to get a feel and hope to see you all at the 50th anniversary meeting!...
