All set for some expanding
09.02.2026 23:35
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All set for some expanding
🧪 We are excited to share this novel open access paper on Phasor Mixing Coefficient to analyze colocalization, developed by folks @i2janelia.bsky.social and @aicjanelia.bsky.social. This is also the first technical paper jointly published with our sister imaging center @malacridalab.bsky.social!
You rock!! Sorry bout that thanks!
Found the unbroken link:
www.biorxiv.org/content/10.6...
@lycasworks.bsky.social developed quantitative expansion (qExM) to estimate endogenous protein quantities in situ. His ExM protocols offer epitope preservation and accessibility so that standard Western Blot antibodies can be used. Also, check out the cristae, supercomplexes, specialized mitos 🤩
🎸Why this is exciting!!🦖
Quantitative super-resolution often requires genetically encoded tags to get labeling sufficient for quantitative imaging. Here with qExM we use commercial antibodies for endogenous targets.
This opens HUGE opportunities to count in tissues, organisms, or clinical samples!
We then used qExM to measure the abundance of mitochondrial respiratory chain complexes in situ!
We measured the abundances for Complex I, III, and IV.
How they differ in absolute abundances in metabolically distinct subpopulations
And even how T-cell activation changes supercomplex density
We validated qExM first on nuclear pore complexes, attempting to quantify the 8 fold symmetry without using this prior knowledge.
We achieved a mean error of just 9.4% 😍🔬🎸
With these tools in place we moved to tackle protein complex abundances that were unknown....
How does qExM work?
We use cryo-fixation + GMA anchoring to boost antibody labeling efficiency, then apply capture-recapture statistics to dual-labeled samples.
The expansion makes epitopes more accessible while reducing label size relative to targets - perfect for quantitative imaging!
Thank you to our amazing group for this project
@sulianamanley.bsky.social
@kmdouglass.bsky.social
@jclandoni.bsky.social
@tittanoferi.bsky.social
@zimmerli.bsky.social
🔬🚨New preprint alert! 🚨🔬
We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology
www.biorxiv.org/content/10.6...
#SuperResolution #CellBiology
PIs & Group Leaders, do your trainees need to learn light microscopy and image analysis? Send them to us! Financial aid is available! Applications due Friday 1/30. meetings.cshl.edu/courses.aspx...
I finally got around to writing the discussion section of our lab's basic training course on live cell #microscopy. I explain the tradeoffs involved in designing an imaging experiment and the art of thinking of the experiment as an optimization problem.
leb-epfl.github.io/basic_traini...
🔬 🖥️ Applications are open for the CSHL course Quantitative Imaging: From Acquisition to Analysis (April 6–21, 2026)!
An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools.
🗓️ Apply online by Jan 30, 2026
Definitely alien 👽
Seal taking a nap off the coast of Cape Town
A super detailed protocol + video on Cryo-ExM - Cryo-Expansion Microscopy, led by the labs of our former postdocs @marinelap.bsky.social & @ebertiaux.bsky.social.
Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...
✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
Thrilled to see our review article "The evolutionary origins of synaptic proteins" highlighted on the cover of Nature Reviews Neuroscience 🤩.
www.nature.com/articles/s41...
@msarscentre.bsky.social 🧠✨🧬🌊🪼🧽
New preprint showing how the membrane-associated periodic skeleton (MPS) restricts endocytosis in neurons: www.biorxiv.org/content/10.6...
It nicely confirms our work from last year (science.org/doi/10.1126/science.ado2032) and extends it to other compartments in more mature neurons
New paper from @zhixingchen2.bsky.social's lab!
It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime!
www.nature.com/articles/s41...
🥳🥳🥳
FLIM at the Biozentrum 🔬
Absolutely, I will!
NHS ester, but I got some post expansion immunolabeling working on the tardigrades I’ll post once I return from this travel
Tardigrades looking BIG
The Company of Biologists 100 logo to the left and QR code to the right. Portrait of Jennifer Waters to the left, text to the right 100 extraordinary biologists Jennifer Waters Jennifer Waters, a FocalPlane Scientific Advisory Board member, is the Director of Core for Imaging Technology & Education, Harvard Medical School, USA. She created MicroList and draws on over 25 years of teaching experience to create accessible microscopy education resources, including the online platform Microtutor. #100biologists #biologists100
We are highlighting Jennifer Waters, a @focalplane.bsky.social Scientific Advisory Board member, Director of CITE, Harvard Medical School, and creator of MicroList (now featured in FocalPlane) and Microtutor, as an extraordinary biologist. #100biologists
@jencwaters.bsky.social
Expansion microscopy of a tardigrade I caught outside with the objective I got on eBay
