**first time together with our (also very fresh) second work (in previous post bsky.app/profile/sfor...), where using mouse genetics we start accumulating evidences going to the same direction: journals.plos.org/plosgenetics...
This project I spearheaded is the fruit of an incredible collaboration between my PhD lab (Matthieu Boulard) and the one of Ana Boskovic, and I could not be more grateful for the supervisors I had and the people I worked with in these 2 labs and at EMBL Rome. ☺️ 4/5
...and finding out that perturbing the level of this nutrient-sensitive modification can change embryonic developmental speed! 3/5
With an enzymatic strategy, we deplete from the mouse preimplantation nuclei an intracellular glycosylation (O-GlcNAc) abundant in the nucleus from the 2-cell stage onwards, studying for the first time (**see 5/5) the effect of O-GlcNAc perturbation on the live growing early mammalian embryo 2/5
A few weeks of delay but still very fresh, the end of an unforgettable chapter: my main PhD paper is in PNAS!!! 🤩 1/5
www.pnas.org/doi/10.1073/...
We were so many. Too special to see all Parisian researchers together...our work is power and together we are powerful!!! #StandUpForScience #StandUpForScienceFrance
@pasteur.fr & its Direction present!!!
@standupforscience.bsky.social @standupforscifr.bsky.social @sufs-paris-rp.bsky.social
I thank again all co-authors, my supervisor Matthieu for making this challenging journey studying O-GlcNAc in vivo so enjoyable, the @ebi.embl.org Rome mouse genome engineering facility (7 mouse alleles!), all colleagues who gave input and also the reviewers of both Review Commons & Plos Genetics 😊
2/3 and start dissecting its embryonic function, which **we also do more in depth using another method in the 2nd paper, hopefully also out in the next months**...so stay tuned 😎
Spoiler: the allelic series suggests a developmental delay upon deglycosylation, which becomes clear in the other paper!
1st 1st-author paper of my PhD (& of my career 😊) is out 🤓 OGT is the only enzyme for reversible intracellular glycosylation in animals. Using a mouse allelic series of hypomorphic OGT-mutants, we discover that intracellular glycosylation is essential for mammalian embryonic development..1/3
