Overall, tRIBO-seq provides a simple way to profile the active tRNAome, capturing abundance, modifications and fragmentation from actively translating ribosomes.
This project was co-led with Hasan Yilmaz, in collaboration between the Novoa Lab, IMMAGINA Biotech and the Soares Lab.
π
Under basal conditions, the ribosome-associated tRNA pool largely matches the total cellular tRNA pool.
But under stress, the picture changes...
Using @nanoporetech.com sequencing allows us to capture multiple layers of tRNA biology in a single experiment:
β’ tRNA abundance
β’ modification signatures
β’ fragmentation patterns
All directly from ribosome-associated tRNAs.
tRIBO-seq builds on RiboLace technology from to pull down active ribosomes.
Using ~15Γ less starting material, we recover comparable ribosome-associated tRNAs to polysome purification - with a much faster protocol.
Importantly, the recovered tRNAs correlate well with polysome-derived samples.
To address this, we developed tRIBO-seq alongside @immagina.bsky.social.
The idea is simple:
β’ capture actively translating ribosomes
β’ isolate their associated native tRNAs
β’ sequence them using nanopore
This provides a direct view of the ribosome-associated tRNA population (ribo-tRNAs).
tRNAs deliver amino acids to ribosomes according to mRNA codons.
But most studies measure total cellular tRNAs, not the tRNAs actually engaged with translating ribosomes.
This makes it hard to understand the role of the active tRNA pool in translation π§.
New preprint from
@novoalab.bsky.social !
Which tRNAs are used by ribosomes during translation?
We introduce tRIBO-seq, a nanopore method to sequence ribosome-associated tRNAs and track how the active tRNA pool changes across stress conditions.
www.biorxiv.org/content/10.6...
Thread π
Using @nanoporetech.com sequencing allows us to capture multiple layers of tRNA biology in a single experiment:
β’ tRNA abundance
β’ modification signatures
β’ fragmentation patterns
All directly from ribosome-associated tRNAs.
tRIBO-seq builds on RiboLace technology to pull down active ribosomes.
Using ~15Γ less starting material, we recover comparable ribosome-associated tRNAs to polysome purification - with a much faster protocol.
Importantly, the recovered tRNAs correlate well with polysome-derived samples.
To address this, we developed tRIBO-seq alongside @immagina.bsky.social
The idea is simple:
β’ capture actively translating ribosomes
β’ isolate their associated native tRNAs
β’ sequence them using nanopore
This provides a direct view of the ribosome-associated tRNA population (ribo-tRNAs).
tRNAs deliver amino acids to ribosomes according to mRNA codons.
But most studies measure total cellular tRNAs, not the tRNAs actually engaged with translating ribosomes.
This makes it hard to understand the role of the active tRNA pool in translation.
tRNAs deliver amino acids to ribosomes according to mRNA codons.
But most studies measure total cellular tRNAs, not the tRNAs actually engaged with translating ribosomes.
This makes it hard to understand the role of the active tRNA pool in translation.
The lab of Single cell ecology has just opened its doors at the University of Glasgow, School of Biodiversity, One Health and Veterinary Medicine!
Join us, collaborate or learn about what we do:
mancinilab.github.io/index.html
And check out our most recent output:
www.biorxiv.org/content/10.1...
It was our pleasure to host @james-bryson.bsky.social (BRIC, Copenhagen) at @crg.eu through the EU-LIFE Postdoctoral Exchange Program!
Big thanks to James for presenting his work on advanced CRISPR tools for epitranscriptomic research, and to @eu-life.bsky.social for this opportunity!π§¬βοΈ
#EULIFE #CRG
π§¬We had a great time at the Genome Biology Program Retreat'25!
Big thanks to the organisers for such an inspiring and fun event π
Congrats to @miemonti.bsky.social for being part of the organising team and to @oguzhanbegik.bsky.social for giving a fantastic talk! π€β¨
#GenomeBiology #CRG #NovoaLab
Novoa Lab joined the PRBB Open Day 2025π¬π§¬π @prbb.org to share our passion for RNA research with the public. Thanks to everyone who came to explore science with us and thanks organisers for making this event possible! #PRBBOpenDay #OpenScience
π¨ New preprint alert π¨
We systematically benchmarked @nanoporetech.com 's modification-aware basecalling models released for RNA on sets of in vitro and in vivo sequences and made some curious observations π§¬π.
bit.ly/4lXqNul
Follow along for a little recap (1/12)
π Weβre excited to share our latest publication in collaboration with Verena Ruprecht's group at @crg.eu
Mechanobiology meets Nuclear Metabolism
www.nature.com/articles/s41...
@embl.org is undoubtedly one of the best place in the world to start your lab! The GB unit is collegial, supportive, collaborative and the science is just outstanding. Apply! DM me if you have questions.
I am so incredibly excited to share our latest work, on the exploration of #RNA secondary structure ensembles and discovery of RNA regulatory structural switches in bacteria and human cells, just out in
@natbiotech.nature.com: nature.com/articles/s41.... A short tread! (1/n)
Last month, the @novoalab.bsky.social attended the Premis Nacionals de Recerca ceremony to celebrate @evamarianovoa.bsky.social receiving the National Young Researcher Award! ππ
Huge congratulations again, Eva - an incredible and well-deserved recognition! π§¬π
Sciencing in the summer
Would you pay $150 per article to post your preprint on @bioRxiv @aRxiv @medRxiv @chemRxiv etc.? Currently itβs free but a small cost could help offset running costs and also work as a barrier against paper mills and AIs just posting all the time and filling these sites up.
I am very happy to have posted my first bioRxiv preprint. A long time in the making - and still adding a few final touches to it - but we're excited to finally have it out there in the wild:
www.biorxiv.org/content/10.1...
Read below for a few highlights...
Endinsaβt en una vivΓ¨ncia sensorial ΓΊnica que connecta ciΓ¨ncia i art immersiu. βSEEING: Un ull i una caixaβ arriba del 5 al 14 de juny al Centre CΓvic Convent de Sant AgustΓ (Carrer del ComerΓ§ 36, Barcelona). www.crg.eu/en/event/see...
Our genome encodes > 7000 microproteins that have been previously ignored in genome annotations. How do these microproteins impact tissue function? Here, we use an in vivo single-cell CRISPR screen to systematically explore microprotein function in the mouse epidermis. 1/9
Scientific research is a strategic necessity for resilience and competitiveness in Europe!
"While the minimum basis for a thriving European Research & Innovation is a stand-alone, well budgeted #FP10, we urge the European Commission to further follow 5 key actions"
lnkd.in/d2mzN_zu
Exciting new work from Barna Lab @stanfordmedicine.bsky.social
www.science.org/doi/10.1126/...
Excited to see issue on ribosome heterogeneity and specialisation out @royalsociety.org Philosophical Transactions B. Thanks to authors & co-editors @liamfaller.bsky.social @andershlund.bsky.social Maria Barna. Wonderful cover Bulat Fatkhullin! #ribosomes royalsocietypublishing.org/toc/rstb/202...
