I bought the coffee that costs $5 less/lb & I just want to say - as a cost-driven consumer, known to be a human garbage disposal: do NOT do it - it is, in fact, yucky!
07.03.2026 02:24
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I bought the coffee that costs $5 less/lb & I just want to say - as a cost-driven consumer, known to be a human garbage disposal: do NOT do it - it is, in fact, yucky!
Really depends what you're looking for. For us we aim for 1 mutaton/ORF and only need to do one round to screen 3-5K colonies - this approaches saturation for an avg size bacterial transcription factor. Get a variety of GOF and LOF, including a few rare.
We use mutazyme II for ORF, assemble into vector or double-cross over product by Gibson, and couple to reporter assay (to detect increase/decrease in expression). Of course bacteria, so easy
Surprised they don't also attempt negging considering the total lack of moral compass:
Dr. Kovács,
You aren't getting any younger & the future looks bleak. Your "friends" will backstab you to stay on top of the funding pile. We care & want to help you, but you have to let us! Please submit...
What happened after the weird Sept 2025 White House press conference on autism?
📉 Tylenol use in the ED dropped >10% among pregnant patients
📈 leucovorin rx jumped 93% among kids 5-17 immediately afterwards
new @thelancet.com today with @jeremyfaust.bsky.social
www.thelancet.com/journals/lan...
🚨Publication alert! In this manuscript we describe the characterization of the non-toxigenic C. difficile (#NTCD) strain L-NTCD03 that we have used in our controlled human colonization experiments. academic.oup.com/femsmicrobes...
Another amazing paper @judithagudo.bsky.social & friends, @nature.com! Metastasis & glucocorticoids 🤯 Check it out!! 👉 doi.org/10.1038/s415... 👇
One of the many wonders of Bacillus
You can have all the unpublished data also. Literally waiting for anyone to need/care
It's good - way better than the pain of hurting myself accidentally! Much more controlled :)
It's awesome - the big belly laughs w/them -totally cracking each other up - is especially great (imo)
Sorry again
Started lifting weights after 30 yrs of not lifting weights. 2 days later, barely able to sit down (yes down): turns out monkey bars & squats are not an equivalent sub. Who knew? 🤷
I actually hate puns, I just cannot help myself. Appalled, jeez
Operation epic optic, now with maganets
@baym.lol this is coming for you - smth to look fwd to
K2 has started doing these really dramatic responses in an English accent when I point out stuff eg. "You forgot to turn the light off"
"Mother, I have failed you!"
Provocative!
Save the date for the next International Conference on Gram-Positive Bacteria which will take place between 29 June–2 July 2027. The organizers (@annadragos.bsky.social, @polonca32.bsky.social, and Ines Mandic-Mulec) look forward to welcoming you to Ljubljana, Slovenia
I could simplify this diagram considerably:
Do you have academic freedom? --> No
This is an incredible resource:
The MicrobeAtlas Database www.cell.com/cell/fulltex...
TLDR: both studies used experimental evolution comparing single & multiple species in biofilms.
They show strong selection to inhabit diverse biofilm niches that single species can do well w/ few mutations, but multiple species can do better, with only 1-2 muts!
❤️collaborators HL Roder🇩🇰 & Moz Hoque
Glob-glob! Absolutely magical sea anemone larva (about 7 mm) from The Lombok Strait, Indonesia 🇮🇩
STEM Black History Fact 28/28
Dr. Jane Cooke Wright was a pioneering cancer researcher and surgeon noted for her contributions to chemotherapy.
cfmedicine.nlm.nih.gov/physicians/b...
On this #raredisease day sharing some amazing work done in #Drosophila uncovering genetic modifiers and therapeutic targets for rare diseases from @clementychow.bsky.social lab @uofubiochem.bsky.social
@the-node.bsky.social
However, more than that, he was an incredible mentor, training dozens of grad students and postdocs, many of whom went on to train another generation of scientists. His impact will continue to be felt for decades to come. I miss him, and his memory is a blessing. 2/
I was sad to learn that my postdoctoral mentor, David Botstein, died yesterday. I started with David as a postdoc in 1998, and he had a profound effect on both my life and scientific career. He was a giant in the field of genetics, making seminal contributions in both yeast and human genetics. 1/
😢 and lovely human
"Mike Cashel: magic spot magician"
journals.asm.org/doi/full/10....
