Schwalbe Lab

Cupriavidus necator, an alternative source for isotopic enrichment of proteins expressed in insect cells for NMR investigations - Journal of Biomolecular NMR Isotopic enrichment of pharmacologically relevant protein targets is crucial for structural studies by nuclear magnetic resonance (NMR) and plays a key role in advancing structure-guided drug discover...

Have a look at our latest publication:
Cupriavidus necator as an alternative source for 15N/13C isotopic enrichment of proteins expressed in insect cells for NMR - enabling structural studies of disease-relevant targets.

Full text link:
link.springer.com/article/10.1...

Prof. Dr. Harald Schwalbe - Schreibgespräch in der Organischen Chemie und Chemischen Biologie YouTube video by Schreibzentrum Frankfurt am Main

Im Youtube-Kanal des Schreibzentrums findet ihr ein Interview von Harald Schwalbe zum Thema "Schreiben in den MINT-Fächern":
www.youtube.com/watch?v=QKFR...

G4 scientists be ready for the next international meeting on G4 structures - G4thering, which I organize with Robert Hänsel-Hertsch and Harald Schwalbe.

At this meeting, G4 related science will be presented and discussed by top scientist. Join us in Frsnkfurt in summer 2027.

The first talk this morning comes from Harald Schwalbe of @goetheuni.bsky.social, Director of Instruct-ERIC, outlining the future directions of structural biology on the global scale.

Find the full paper here - instruct-eric.org/news/the-fut... @schwalbe-lab.bsky.social @itqbnova.bsky.social

Less missing values—evaluation of proteomics workflows for the quantification of (small) proteins This study systematically evaluates methods for improving the identification and quantification of small proteins using bottom-up mass spectrometry, thereb

New paper out in microLife: evaluating proteomics workflows to reduce missing values, with a focus on reliable quantification of small proteins. Practical insights for improving coverage and data quality.

academic.oup.com/microlife/ar...

#Proteomics #MassSpectrometry #SmallProteins #Microbiology

Fragment-Screen coordinator Harald Schwalbe is presenting on NMR screening and the outcomes of the project at the @eu-openscreen.bsky.social Impulse Winter Training School!

Find out more about the meeting and Fragment-Screen here - fragmentscreen.org/winter-train... @schwalbe-lab.bsky.social

Great to see that the Instruct-ERIC review of the future of integrated structural biology is continuing to be highlighted!

Find out more about the paper and read here - instruct-eric.org/news/the-fut... @schwalbe-lab.bsky.social

DNP-Enhanced Magic Angle Spinning Solid-State NMR Spectroscopy to Determine RNA–Ligand Interactions Understanding the molecular recognition underlying RNA–ligand complex formation is of key importance to explain the RNA regulatory function of riboswitches and to support the development of low-molecu...

DNP-Enhanced Magic Angle Spinning Solid-State NMR Spectroscopy to Determine RNA–Ligand Interactions | Journal of the American Chemical Society pubs.acs.org/doi/10.1021/...

Crystal structure of the class V GTP-binding RNA aptamer bound to its ligand: GTP recognition by a topologically complex intermolecular G-quadruplex Abstract. RNA aptamers obtained from in vitro selection experiments provide unique insights into the structural properties and ligand recognition capabilit

Check out the newest publication of the Wöhnert lab! ☺️
"Crystal structure of the class V GTP-binding RNA aptamer bound to its ligand: GTP recognition by a topologically complex intermolecular G-quadruplex"

Full text: academic.oup.com/nar/article/...

#G4 #X-ray #aptamer #NAR

Integrative In Silico and In Vitro Screening of Low Molecular Weight Compounds Targeting SARS‐CoV‐2 RNA Elements A combined virtual and nuclear magnetic resonance (NMR)-based screening approach identifies small molecules that bind conserved SARS-CoV-2 RNA structures. Diverse compound libraries are screened usin...

New publication alert 🎉
Our latest paper explores how small molecules can bind SARS-CoV-2 RNA, combining in silico screening with NMR experiments to uncover promising RNA-targeting candidates.

full text: chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...

#RNA #NMR #screening #drugtargeting

Lucia Banci, Instruct-IT, and Harald Schwalbe visit the new Instruct-DE research side at TU Munich.
Lucia covers the technological developments in drug screening by in-cell NMR, supported by Fragment-Screen

fragmentscreen.org @schwalbe-lab.bsky.social @cerm-cirmmp.bsky.social @tumunich.bsky.social

At this years German User Meeting of Bruker, Instruct Director Prof. Harald Schwalbe introduced the Access options in Instruct for structural biology to more than 200 NMR Scientists, including many facility managers from Germany, Austria and Switzerland - instruct-eric.org @schwalbe-lab.bsky.social

The Zuo1 C-terminal domain stabilizes DNA guanosine quadruplex (G4) structures located on Chromosome IX in Saccharomyces cerevisiae Abstract. Deoxyguanosine quadruplexes (G4s) form stable non-B-DNA structures that can affect transcription, replication, and genome stability. Depending on

Our work on DNA G4 interactions with Zuo1 in collaboration with @paeschkelab.bsky.social and the Penedo lab is out in @narjournal.bsky.social 😍

Full text: academic.oup.com/nar/article/...

#G4 #G-Quadruplex #NMR #smFRET #ChIP-qPCR

Structure‐Based Rational Design of a Selective Hydrolase Inhibitor of the Severe Acute Respiratory Syndrome Coronavirus‐2 Nsp3 Macrodomain Structure-guided design of a sulfamoyl-modified analog of GS-441524 yielded a selective, high-affinity inhibitor of the SARS-CoV-2 macrodomain Nsp3b. The compound occupies the phosphate-binding pocke...

Have a look into our newest publication showing how GS-441524 derivatives can potently inhibit the SARS-CoV-2 macrodomain (Nsp3b), offering a structural roadmap for next-generation antiviral design.

chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...

#SARSCoV2 #NMR #drugtargeting #synthesis

Prof. Harald Schwalbe presenting Fragment-based Drug Discovery at at the 25th ChemBioChem Symposium!

Prof. Schwalbe is coordinator of the Fragment-Screen project, developing new tools for fragment-based drug discovery

Find out more here - fragmentscreen.org/home @schwalbe-lab.bsky.social

Professor Harald Schwalbe Renewed as Instruct-ERIC Director Instruct-ERIC is a pan-European research infrastructure in structural biology, making high-end technologies and methods available to all European researchers.

Great news: Harald Schwalbe has been reappointed as Director of Instruct-ERIC, extending his term through Dec 2031. His leadership shows strong confidence in his work advancing structural biology and research collaboration across Europe. @instruct-eric.bsky.social
instruct-eric.org/news/profess...

Structural role of conformational heterogeneity and juxtamembrane regions in the Ephrin A1 interactions with the EphA2 receptor ligand-binding domain Ephrin growth factors are attached to the membrane by either GPI-anchor or transmembrane domains and are known to signal bi-directionally: cells, expr…

Our study shows Ephrin A1 undergoes conformational selection when binding EphA2, while its juxtamembrane regions stay flexible. These insights clarify bidirectional signaling and may guide future therapeutic strategies.
Full text: www.sciencedirect.com/science/arti...

#NMR #Ephrin #Cancer #receptor

Noncanonical RNA binding of human La-related protein 6 Abstract. La-related proteins (LARPs) are RNA-binding proteins that are involved in a variety of disease-related processes. Most LARPs recognize short sing

Great contribution from our former lab member Robert Silvers in @narjournal.bsky.social ☺️
"Noncanonical RNA binding of human La-related protein 6"
Full text: academic.oup.com/nar/article/...
#LARP #NMR #RNA #structure

Dimerization propensity of the β1-adrenergic receptor in lipid nanodiscs probed by DEER and single-molecule spectroscopies | PNAS G protein–coupled receptors (GPCRs) comprise a large class of membrane proteins that mediate cellular responses to a wide range of external signals...

Take a look at the publication of our former lab member Nina Kubatova in @pnas.org 😇
"Dimerization propensity of the β1-adrenergic receptor in lipid nanodiscs probed by DEER and single-molecule spectroscopies"
Full text: www.pnas.org/doi/10.1073/...
#DEER #protein #SingleMolecule #GPCR

Together with the Evanseck Lab, our lab just published the NMR + MD structure of the Omicron SARS-CoV-2 s2m RNA, revealing a pentaloop with remarkable dynamics.

Full text: m.rnajournal.cshlp.org/content/earl...

#NMR #MD #RNA #SARSCoV2 #structure #dynamics

At the 2025 meeting of the German focus group of Magnetic Resonance (FGMR, EPR/NMR), Harald Schwalbe, Instruct-director, introduced access possibilities and highlighted the recent installation of the German Instruct centre.

@instruct-eric.bsky.social @gdch.de

#NMR #EPR #FGMR #GDCh #InstructERIC

Structural basis for ligand recognition in the tobramycin riboswitch Abstract. Recently, a novel tobramycin-responsive riboswitch was developed by a combination of Capture-SELEX and in vivo screening. This riboswitch regulat

Exciting work from Duchardt-Ferner et al. in NAR: high-res. NMR structure of a tobramycin-responsive riboswitch. Shows a novel aminoglycoside-binding RNA motif with unique RNA-RNA contacts, explaining its exceptional switching efficiency in eukaryotic translation.
academic.oup.com/nar/article/...

Instruct Director Harald Schwalbe will be in Denmark for the SBiNLab’s 25th anniversary!

www1.bio.ku.dk/english/rese... @schwalbe-lab.bsky.social

Researchers in Denmark can receive access to Instruct technologies and instrumentation here - instruct-eric.org/submit-propo...

Targeting the SARS-CoV-2 RNA Translation Initiation Element SL1 by Molecules of Low Molecular Weight We present the development of low molecular weight inhibitors that target the 5′-terminal RNA stem-loop 1 (SL1) of the SARS-CoV-2 genome. SL1 is crucial for allowing viral protein synthesis in the con...

Our newest publication is online!
Using fragment-growth guided by NMR spectroscopy, we optimized small molecules targeting SCoV-2 SL1 RNA, integrating solubility, affinity, and specificity to identify translation inhibitors.

Full text: pubs.acs.org/doi/10.1021/...

#NMR #drugdesign #RNA #SARSCoV2

1H, 13C and 15N chemical shift assignment for stem-loop 5a from the 5‘UTR of HCoV-229E - Biomolecular NMR Assignments Due to the emergence of the SARS-CoV-2 virus, research on coronaviruses has been massively accelerated. In addition to SARS-CoV-2, there are other human coronaviruses, including HCoV-229E. In all coro...

Check out our newest chemical shift assignment! 🎉
We used NMR to characterize the SL5a stem-loop from HCoV-229E, a conserved RNA element key to coronavirus RNA translation and replication.

doi.org/10.1007/s121...

#RNA #NMR #Virology #StructuralBiology #Assignment #Coronavirus

RT @euromar2025 EUROMAR 2025 opens in Oulu A fantastic lineup of prize winners: 🏆 Prof. Thomas Prisner – Richard R. Ernst Prize 🏆 Dr. Nergiz Sahin Solmaz – Raymond Andrew Prize 🏆 Prof. Tuo Wang – Ampere Prize for Young Investigators

RT @euromar2025 EUROMAR 2025 opens in Oulu
A fantastic lineup of prize winners:
🏆 Prof. Thomas Prisner – Richard R. Ernst Prize
🏆 Dr. Nergiz Sahin Solmaz – Raymond Andrew Prize
🏆 Prof. Tuo Wang – Ampere Prize for Young Investigators

Congratulations to all!
#EUROMAR2025 #NMR #EPR #MRI #ESR #NMRchat

Workshop Alert: On July 10, Nathalie Weickgenannt and Laura Buglioni from @angewandtechemie.bsky.social will be at Goethe University Frankfurt to host a Wiley Publishing Workshop. Don’t miss this opportunity to gain valuable insights into scientific publishing!

FEBS Press Papain-like protease from SARS-CoV-2 plays an important role in the cleavage of the viral polyproteins and in the suppression of the host's immune response. Here, we present the results of an NMR scr...

New publication is out in @febsopenbio.bsky.social ! 🎉
"NMR screening of low molecular weight inhibitors targeting the papain-like protease (PLPro) of SARS-CoV-2"

Full text: febs.onlinelibrary.wiley.com/doi/10.1002/...

#screening #NMR #SARS-CoV2 #targeting

Structural heterogeneity and dynamics in the apical stem loop of s2m from SARS-CoV-2 Delta by an integrative NMR spectroscopy and MD simulation approach Abstract. In structured RNAs, helical elements are often capped by apical loops that are integral structural elements, ranging from 3 to >20 nts of size

New publication in @narjournal.bsky.social - Using NMR, SAXS & MD, we characterize the dynamic nonaloop of the SARS-CoV-2 Delta s2m RNA, contributing to future ensemble-functional studies of dynamic RNA motifs.

Full text: doi.org/10.1093/nar/...

#RNA #StructuralBiology #NAR #NMR #MDSimulations

Lucio Frydman Lab on X: "Destruction was extensive, but thanks to numerous precautions there were zero casualties. The Gigahz, the 7T human, the 15.2T animal and the DNPs, are all up and running. So is the He recycling, and our lower-field 500, 600 solids and liquids machines. So life goes on! https://t.co/vaxW9hx8WP" / X Destruction was extensive, but thanks to numerous precautions there were zero casualties. The Gigahz, the 7T human, the 15.2T animal and the DNPs, are all up and running. So is the He recycling, and our lower-field 500, 600 solids and liquids machines. So life goes on! https://t.co/vaxW9hx8WP

x.com/lab_lucio/st...