Falk Schneider

🤩

New preprint out!
In this study, we introduce NanoFLex, a strategy that combines #HaloTag variants with #nanobodies -based immunolabeling to enable rapid, OneStep-IF #lifetime #multiplexing. Possible due to SmartSecondaries fused to #HaloTag from @nanotag.bsky.social: www.biorxiv.org/content/10.6...

𝗘𝘅𝗰𝗶𝘁𝗶𝗻𝗴 𝗗𝗲𝘃𝗲𝗹𝗼𝗽𝗺𝗲𝗻𝘁𝘀 𝗶𝗻 𝗠𝗶𝗰𝗿𝗼𝘀𝗰𝗼𝗽𝘆 𝗥𝗲𝗽𝗼𝗿𝘁𝗶𝗻𝗴! If you're involved in creating, processing, or publishing microscopy data, take a look at the new checklist for reporting requirements provided by @quarep-limi.bsky.social in @jcb.org 🔗 rupress.org/jcb/article/...

Oooooh! Looking forward to reading this one! The first author is the delightful Aviya Perlman Illouz, working with Raya Sorkin. They used a clever system of a phase-separated supported bilayer on a PDMS substrate that they stretched in 2 directions.

Intelligent Imaging Innovations - Microscopist Intelligent Imaging Innovations

3i is hiring a Microscopist - repost & tell a friend!
Ideal candidate has experience in fundamental optical theory & familiarity with lightsheet, spinning disk, multiphoton, TIRF etc. Full-time, exempt position (travel to visit customers) offering a competitive salary & comprehensive benefits.

When vessel constriction fails: Cellular mechanics linking developmental remodeling to vascular malformations

Happy to share a write-up of our recent publication @natcomms.nature.com demonstrating
how circumferential actomyosin-driven endothelial cell contractions constrict vessel diameter, providing insights into mechanisms of normal vessel remodelling and CCM.
www.bdr.riken.jp/en/news/rese...

Just published: chemotactic sperm cells become phototactic 💡- by loading photo-activatable second messengers. This allows to "reverse-engineer" chemotactic signaling dynamics and expose cells to signals they would never see in the wild. Honoured to be part of this collaboration with the Kaupp lab.

hey team! I need advice! If there were theoretically an organization whose sole purpose was to systematically identify and break nuisance and stupid IP in the biomedical space, creating shareware equivalents, what would be the highest value targets? e.g., AAV serotypes, antibodies, CRISPR, 1/n

Spatial patterning of contractility by a self-organized mechanogen activity gradient underlies Drosophila gastrulation - Nature Communications During morphogenesis patterned contractility drives tissue shape changes. Here they show that GPCR signaling and integrin activation give rise to a dynamically translocating gradient of contractility ...

New paper out! In this project, we focused on the question how tissue mechanics is spatially controlled during development. We show that the GPCR ligand Fog acts as a “mechanogen” during Drosophila gastrulation, driving a self-organized wave of tissue invagination:
www.nature.com/articles/s41...

A photo of Lynmarie Thompson, the president of the Biophysical Society, awarding a plaque to Erdinc Sezgin. They are both smiling.

Congratulations to @sciezgin.bsky.social for being honored with the 2026 Early Independent Career award from the @biophysicalsoc.bsky.social!

The Lipid Interactome: an interactive and open access platform for exploring cellular lipid–protein interactions AbstractSummary. Lipid–protein interactions play essential roles in cellular signaling and membrane dynamics, yet their systematic characterization has lon

This is SO COOL!!!

academic.oup.com/bioinformati...

Xiaofeng combined 2D live-cell single-particle tracking with 3D simulations to specifically measure the bacterial nucleoid accessibility and viscosity! Our preprint describes how these nucleoid properties respond to cellular processes! Code is available too.
www.biorxiv.org/content/10.6...

Lipid geeks rise up!

The magnetic field-dependent fluorescence of MagLOV2 in live bacterial cells is consistent with the radical pair mechanism https://www.biorxiv.org/content/10.64898/2026.02.18.706690v1

Tissue phase transitions in development: more than just mechanics Summary: Tissue material phase transitions are classically thought to regulate tissue deformability. This Review emphasises their unexpected roles in directly influencing growth and patterning signall...

Excited to share our new review in @dev-journal.bsky.social on tissue phase transitions during development!

@karengrace12.bsky.social @nicolettapetridou.bsky.social

🔗 doi.org/10.1242/dev....

The Biochemical Society Awards 2025. Biochemical Society Award - Sustained Excellence. Professor Rob Cross, University of Warwick. Includes Biochemical Society awards logo and a photo of Professor Cross.

See @irobcross.bsky.social at Dynamic Cell VI, as he presents his Biochemical Society Award for Sustained Excellence Lecture on the interlocking nanomechanics of kinesins and tubulins! Register now: bit.ly/Dynamic-Cell... #mechanobiology

Learning the Principles of T Cell Antigen Discernment T cells are central to the adaptive immune response, capable of detecting pathogenic antigens while ignoring healthy tissues with remarkable specificity and sensitivity. Quantitatively understanding h...

Happy to share our latest review article, “Learning the Principles of T Cell Antigen Discernment.” www.annualreviews.org/content/jour...
with @fxpbourassa.bsky.social , Sooraj Achar and Grégoire Altan-Bonnet ! 1/4

New method paper out today on FLIM analysis with Flipper-TR.
We discuss fitting strategies, common pitfalls, and quantitative interpretation for measurements.
First corresponding/last authorship for me!
Free link:
authors.elsevier.com/a/1me2hHRzCb...
Thanks to @rouxlab.bsky.social and Tithi Mandal!

A model to describe what we show in the paper. INVs are important for sequestering GLUT4 and related proteins into GSVs, the vesicles that are mobilised in response to insulin.

🚨 We have a new preprint out! 🧪

In this paper we look at GLUT4 trafficking. This is a short explainer thread, but please read the paper!

1/n 🧵

www.biorxiv.org/content/10.6...

Local Confinement within Plasma Membrane Nanodomains Drives Constitutive Activity of GPCRs https://www.biorxiv.org/content/10.64898/2026.02.17.706370v1

GitHub - imaging-formats/bffile: Modern Bio-Formats wrapper with clean lazy Python API Modern Bio-Formats wrapper with clean lazy Python API - imaging-formats/bffile

Rewrote my Python bioformats wrapper (from aicsimageio/bioio) as a standalone package:

- fully bootstrapped Java setup (just pip install)
- lazy, repeatably-indexable Array object
- fully spec-compliant OME-Zarr group obj.
- xarray/dask exports

github.com/imaging-form...
v0.0.rc1 on PyPI

Phasor analysis of RGB camera data enables fluorescence microscopy unmixing and brightfield segmentation in a commercial microscope https://www.biorxiv.org/content/10.64898/2026.02.13.705652v1

#ZebrafishZunday: Valentine's Day Edition ❤️ Beating heart of a transgenic zebrafish embryo. Credit to @zebrafish007.bsky.social. 🧪

We are finally online 🥳 What a fantastic adventure with @rrsims, the @emilianilab.bsky.social and all co-authors from IdV Paris #TwoPhoton #Voltagers #Holography #Rhodopsins

🚨🚨Double preprint alert! 🚨🚨
Two papers went up on Biorxiv this week!
The first presents new chemigenetic FRET-based biosensors: www.biorxiv.org/content/10.6...
And the second presents an improved version of our C-Y FRET PKA reporters, AKAR6 www.biorxiv.org/content/10.6...
Thread 🧵 1/5

Finally putting my social media skills to work for my favorite societies 💪

Happy to announce I've joined the IZFS Publications & Communications Committee to coordinate their member news for the News Splash!

I've also taken on the role of Co-Chair of the newly-formed ZDMS Communications Committee!

Inducing novel endosymbioses by implanting bacteria in fungi - Nature A study presents an approach to establish and track a new endosymbiotic partnership by implanting bacteria in a non-host fungus and shows that stable inheritance of the implanted bacteria is possible ...

Vorholt Lab re-created endosymbiosis in the lab: injected bacteria into a fungus and evolved a heritable partnership.

Evolution, fast-forwarded.

doi.org/10.1038/s415...

How do biomolecular condensates🫧 interact with membranes? We show that dielectric permittivity contrast between coexisting phases directly governs condensate wetting behavior.

👉 preprint: bit.ly/4qBmVRx

Permittivity matters.🧪🫧

The CIC furthermore hosts @eva-kreysing-lab.bsky.social Darius Köster Amit Singh Vishen and of course Jitu Mayor.

We'll make an effort to get everyone on bluesky and keep you posted on the science 🔭🧪