TJ Krzystek

We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji.

It ships many new features, detailed below, but that are articulated around the following:

A schematic representation of HTT’s multifunctionality in the autophagy–lysosome pathway. HTT interacts with ULK1, aiding its recruitment to donor membranes containing PI(4,5)P2 and ATG9, leading to phagophore nucleation, and LC3–I conjugation to LC3–II. HTT can interact with p62 for cargo recruitment of cellular debris, damaged organelles, and ubiquitinated proteins. Subsequently, HTT associates with autophagosomes and late endosomes, likely acting in concert with RAB7 for microtubule–dependent transport to lysosomes following STX17–mediated fusion. Finally, HTT is implicated in lysosome fusion with both autophagosomes and amphisomes for the degradation of cellular cargo.

Pleased to share my latest review article with @tandfresearch.bsky.social, taking a deep dive into neuronal autophagy, how Huntingtin coordinates it, and how the process goes awry in Huntington’s disease.

doi.org/10.1080/27694127.2025.2472450

#Neuroscience #Autophagy #HuntingtonsDisease

Opposing roles for GSK3β and ERK1-dependent phosphorylation of huntingtin during neuronal dysfunction and cell death in Huntington’s disease - Cell Death & Disease Cell Death & Disease - Opposing roles for GSK3β and ERK1-dependent phosphorylation of huntingtin during neuronal dysfunction and cell death in Huntington’s disease

Always exciting when a dissertation chapter makes it to publication.

Thrilled to share this work in Cell Death & Disease, exploring putative roles of GSK3β and ERK1 phosphorylation in Huntington’s disease.

Full article: doi.org/10.1038/s414...

Huge thanks to all co-authors & contributors! 🦾

#GoBills

Do you want to hear the latest and greatest about the neuronal cytoskeleton? Join us in Chile next April for #NeuroCyto2025, organized by @monicamsousa.bsky.social, Kassandra Ori-McKenney and Tomás Falzone! Registration is open until March 5: neurocytoskeleton.com/2025/

Exciting work! 🎉

appreciate the compliment, Rachael!

caption/alt:

iPSC-derived spinal motor neurons from a control/healthy individual were aged 32 days, probed with Spirochrome's pkMito mitochondria dye, then assayed 24hrs later on our automated imager.

The sequence depicted was sped up to 20fps,
but images were capture every 4s across 5mins.

Likely my final #FluorescenceFriday post of 2024! 🔬

Here’s a kinetic showcasing pkMito dynamics and the heterogeneity of axonal mitochondria

I'm looking forward to all of the incredible microscopy from this community in 2025.

Happy Holidays everyone! ☃️❄️

I can watch the ebb-and-flow of that leading edge all day!

That'll be a really interesting comparison! Mitochondria movement is quite challenging to predict with shorter timelapses like this.

Not sure how much it applies beyond axonal transport, but lysosomes and other vesicles always give me less noisy and more linear particle tracking data.

Much appreciated 🫡

Some #MicroscopyMonday to kick off the week! 🔬⛄

I paired #lysosome and #mitochondria kinetics side-by-side to highlight their distinct axonal motility.

I thought this nicely shows the need for organelle-specific metrics when designing high-throughput screening assays.

#Neuroscience #CellBiology

It's also #FluorescenceFriday 🔬
so, here's a taste of the lysosomal transport you can find at my poster (HCB-23)

At TDI, we've been integrating #microscopy and #phenomics to identify and validate phenotypes of ALS patient-derived spinal motor neurons for subsequent drug screening.

At HCB-24,
Anushka B. will share a novel TDP-43 KO cell line for disease modeling.

At HCB-14,
Therese D. will share how C9orf72 HRE or LoF impacts TDP-43 mis-splicing.

At HCB-23,
I'll share our design & development of an automated, live-cell imaging pipeline for drug screening and phenomics.

The cell biology team at ALS Therapy Development Institute will be sharing some exciting work today at the 2024 International Symposium on ALS/MND. #endALS #Neuroscience #CellBiology

If you're here in Montreal, come chat with us during theme05: human cell biology and pathology (details below): 🧵

Important article that challenges the classic view of axons as simple tubes. To what extent they fluctuate in diameter is up for debate - see some thoughts in this commentary piece in @science.org. Exciting time for axon scientists!

I curated more new works here: biomed.news/bims-axbals/...

This collection focuses on recent studies related to axonal biology, cell biology of neurons, neurodegenerative diseases, and some iPSC biology.

@gavinmcstay.bsky.social @biomednews.bsky.social

#Neuroscience #CellBiology #AxonalBiology

Axonal mRNA studies always catch my attention.
Great preprint from the Redemann and Hermann labs: www.biorxiv.org/content/10.1...

". . . only very few studies have paid attention to the important roles of distinct mRNA expression profiles of the axon and soma . . ." #MoreAxonSeq #Neuroscience

ImageJ Fiji 💯

Adding setBatchMode(true) saves a lot of time if you're using a custom macro/script.

I've been processing tifs from 384-well plates with 180-360 frames/well.

The alt text disappeared on me 😱

Here it is:

iPSC-derived motor neurons (labeled green with a membrane dye) were treated with four concentrations of an exogenous stressor (0uM, 0.01uM, 0.1uM, 1.0uM) and imaged every 6 hours across 54 hours using our automated spinning disk.

Scale = 100microns

Sharing #FluorescenceFriday early before the food coma hits this weekend! 🦃

Here are longitudinal kinetics showing dose-dependent degeneration from an exogenous stressor: part of an assay developed for drug screening with iPSC-derived neurons. 🔬

#Microscopy #Neuroscience #Bioimaging #CellBiology

I just created a Microscopy feed that tracks anything that has a cute 🔬 emoji on it as well as the simple #microscopy and #imaging tags. Enjoy!
bsky.app/profile/did:...

Fall hikes 😌

...terrifying

During my postdoc I started running a lot of over-night/weekend live-cell imaging . . . and discovered a whole new fear of drift I never knew I had ☠️

That's ridiculous, sorry 😒

Interested in microscopy and/or image analysis? Check out my newest starter pack of super folks.(which also links to the previous, 150-awesome-full one! Both also link to other imaging-y starter packs as I find them)

go.bsky.app/MU8c2A2

FocalPlane features... reproducibility in imaging YouTube video by The Company of Biologists

ICYMI: The October FocalPlane features… webinar included talks from @helenajambor.bsky.social (‘How not to lie with image data’) and Kota Miura ('Reproducible bioimage analysis for ensuring #scientificintegrity').

Watch the recording at
bit.ly/4fyB2SU

#PublicationIntegrityWeek #COPE

My turn to create a starter pack for scientists using live imaging in their research! Of course, I’ve likely missed many—please let me know who else I should include. Thanks!
go.bsky.app/KYYr7xc
#liveimaging #livevellimaging #microscopy