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Matti Wahlsten

@matwah

Microbiology (cyanobacteria, lichen and fungi), natural products and mass spec in University of Helsinki.

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Posts 11.11.2024
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Latest posts by Matti Wahlsten @matwah

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Neptunizhulides, Cryptic trans-AT Polyketide Synthase-Derived Metabolites from Paraneptunicella aestuarii NBU2194 Genome mining of Paraneptunicella aestuarii NBU2194 resulted in the identification of a family of 17-membered macrolides, neptunizhulides A–F. Their structures were elucidated by comprehensive spectroscopic data analysis. Stereochemical assignments of the neptunizhulides were determined by J-based configuration analysis, ROESY NMR, Mosher’s ester derivatization, and bioinformatic predictions. Bioinformatic analysis and a 15N-serine labeling experiment resulted in the identification of a putative trans-acyltransferase polyketide synthase (trans-AT PKS) biosynthetic gene cluster, and a biosynthetic pathway is proposed.

Thanks for the co-authors for the dive into the trans-AT pks world.

pubs.acs.org/doi/10.1021/...

#microsky

09.01.2025 10:01 👍 3 🔁 1 💬 0 📌 0
Preview
Neptunizhulides, Cryptic trans-AT Polyketide Synthase-Derived Metabolites from Paraneptunicella aestuarii NBU2194 Genome mining of Paraneptunicella aestuarii NBU2194 resulted in the identification of a family of 17-membered macrolides, neptunizhulides A–F. Their structures were elucidated by comprehensive spectroscopic data analysis. Stereochemical assignments of the neptunizhulides were determined by J-based configuration analysis, ROESY NMR, Mosher’s ester derivatization, and bioinformatic predictions. Bioinformatic analysis and a 15N-serine labeling experiment resulted in the identification of a putative trans-acyltransferase polyketide synthase (trans-AT PKS) biosynthetic gene cluster, and a biosynthetic pathway is proposed.

Thanks for the co-authors for the dive into the trans-AT pks world.

pubs.acs.org/doi/10.1021/...

#microsky

09.01.2025 10:01 👍 3 🔁 1 💬 0 📌 0