Conference season is back! ๐ฌ๐งช
Weโre excited to return to Novรฉ Hrady for the XXI Discussions in Structural Molecular Biology by @structbiocssb.bsky.social.
Closing the final session, Pavel Plevka dived into the fascinating world of #enterovirus entryโa great way to wrap up the meeting!
Hi, can our lab be added to the pack? We study interaction of phages with biofilm ๐
Hi, can our lab be added to the pack? ๐
Hi, can our lab be added to the pack? ๐
Hi, can our lab be added to the pack? ๐
Hello, can you add our lab to the pack? :)
Can you, please, add our lab to the list? ๐
Can you, please, add our lab to the list?
We would like to be added to the list ๐
๐
Thank you, Lars! Weโre thrilled to join this vibrant community and connect with amazing labs like yours. Looking forward to engaging discussions and collaborations in structural virology!
A starter pack with science institutions and research labs in Czech Republic.
Let us know what is missing!
go.bsky.app/Bjam2aQ
โ๏ธ Enjoying the first and, hopefully, not the last snow of this winter โ๏ธ
Stay tuned for more insights into viral entry mechanisms! ๐งซ
If you liked this #tweetorial, please retweet the first tweet.
bsky.app/profile/plev...
Endosome rupture enables enteroviruses from the family Picornaviridae to infect cells
nature.com/articles/s42...
#CEITECScience
9/9
๐ช๐ต๐ ๐ฑ๐ผ๐ฒ๐ ๐๐ต๐ถ๐ ๐บ๐ฎ๐๐๐ฒ๐ฟ?
This study brings us closer to the fundamental understanding of the infection process.
8/9
๐ ๐ฒ๐บ๐ฏ๐ฟ๐ฎ๐ป๐ฒ ๐๐ฎ๐ฟ๐ฝ๐ถ๐ป๐ด ๐ฎ๐ป๐ฑ ๐ฒ๐ป๐ฑ๐ผ๐๐ผ๐บ๐ฒ ๐ฟ๐๐ฝ๐๐๐ฟ๐ฒ
Actin filaments observed at membrane protrusions, along with actin polymerisation inhibition results suggest cellular actin can drive endosome rupture.
7/9
On the contrary, inhibition of endosome acidification didnโt stop genome-containing particles from exiting the endosomes.
6/9
๐๐ฎ๐ป ๐ฐ๐ฒ๐น๐น๐๐น๐ฎ๐ฟ ๐บ๐ฒ๐ฐ๐ต๐ฎ๐ป๐ถ๐๐บ๐ ๐ฏ๐ฒ ๐ถ๐ป๐๐ผ๐น๐๐ฒ๐ฑ? ๐ฆ
Inhibition of the actin polymerisation led to genome release within the endosomes, hindering the virus replication.
5/9
๐๐ผ๐ ๐ฑ๐ถ๐ฑ ๐๐ต๐ฒ๐ ๐ฑ๐ผ ๐ถ๐?
Using advanced #cryoET at #CEITEC cryoEM facility
to visualise cell entry of rhinovirus 2, echovirus 18, echovirus 30, and enterovirus 71 โ right in action! ๐
4/9
๐๐ฒ๐ ๐๐ถ๐ป๐ฑ๐ถ๐ป๐ด๐
This study reveals that enterovirus-containing #endosomes can deform and rupture, enabling the release of genome-containing particles into the cytoplasm.
3/9
#Enteroviruses, members of the Picornaviridae family, cause illnesses ranging from the common cold to encephalitis ๐ท However, the infection process was never observed ๐ช๐ฏ ๐ท๐ช๐ท๐ฐ - until now.
2/9
โจ๐ก๐๐ช ๐ฃ๐๐ฃ๐๐ฅ ๐๐๐๐ฅ๐งโจ
We are happy to announce that the paper by Aygul Ishemgulova and members of our group, describing the novel mechanism of cell invasion by enteroviruses is now published in @commsbio.bsky.social ! ๐ฆ
Letโs break it down!
๐งต
1/9
Hello Bluesky!
We're the Plevka Lab, a structural virology research group at CEITEC. Using techniques like SPA #cryo-EM and #cryo-ET ๐ฌ, we explore the fascinating structures of viruses to uncover their secrets. Follow us for research updates, insights, and a glimpse into the world of #virology! ๐งซ ๐ฆ
