Benjamin Grädel

Figure legend: Categories and capabilities of smart microscopy systems integrating real-time image analysis and feedback control. Top: Smart microscopy workflows can be classified based on the driving logic behind decision-making: Event-driven (reacting to rare biological events), Outcome-driven (using feedback-control to steer biological systems toward a desired state), Quality-driven (optimizing signal quality or imaging metrics), and Information-driven (guided by models that predict which measurements/perturbations will yield the most informative data). Middle: Central feedback loop between the microscope and an image analysis system, which continuously exchanges images and commands to guide acquisition dynamically. Bottom: Key control actions enabled by smart microscopy: adjusting imaging modality (e.g. switching from brightfield to fluorescence, adjusting sampling rate), repositioning the field of view (e.g. tracking, drift correction), optimizing acquisition settings (e.g. adaptive optics), and performing photomanipulation (e.g. FRAP, ablation, optogenetics).

🔬🧠 Our paper on smart microscopy & the issue of interoperability! www.biorxiv.org/content/10.1... LONG THREAD WARNING: Smart microscopy uses real-time image analysis to automatically guide the acquisition or perturbation of the sample (closed feedback-control loop). Many applications exist:

Automated optogenetic control of hundreds of cells in parallel. Each cell is individually steered, collectively acting as a "tissue printer". Preprint & code out! www.biorxiv.org/content/10.1...

PREPRINT OUT ✨ "hack" your microscope to work as a 3D printer! print micrometer sized features at cm scale. a method for easy and cheap microfabrication, with a focus on biological applications. THREAD ↓
www.biorxiv.org/content/10.1...

Time to build up my network after switching here 😁

It's the occasion to share one of my older images for #FluorescenceFriday here!

The image shows a massive Boa Constrictor cell culture syncytium with cell membranes in gold, microtubules in cyan and nuclei in grey:)

#sciart

Now that we’re on Bluesky, it’s a good time to bring back #badsciencedrawings – a collection of figures that prove that science is more science than art.
Before Biorender, all we had was MS Paint and a dream. But ovals, lines, and lightning bolts were all we needed

Morales-Botello et al., 2012

🚀 It's #FluorescenceFriday, and commissural axons are coming at you!

🧪🔬 15-hour time-lapse of chick commissural axons navigating and crossing the CNS midline (and reverse).

One stack taken every 10 minutes. Pixels were color-coded in the z-axis.

#LiveImaging #Neuroscience #Science #Microscopy 🧠✨

🔬 Calibrating the DMD for optogenetics. Project light on your cells by drawing in napari-pymmcore!

Mix two motors of opposite polarity with microtubules and they will partition space !
Microtubules will get organized in polar and active barriers, sorting the two motors in separated domains, leading to the emergence of a new type of patterns.
#morphogenesis
doi.org/10.1073/pnas...
(1/n)

Chemo-mechanical diffusion waves explain collective dynamics of immune cell podosomes - Nature Communications Dendritic cells can utilize the dynamics of podosomes to probe their microenvironment. Here, the authors propose a chemo-mechanical model for the height oscillations of individual podosomes and the co...

Maybe somewhat related to this www.nature.com/articles/s41...

I think they originate from the actin spots that grow then collapse and send out a wave, then grow again. with many actin spots next to each other you get complex patterns. But this is purely guessing on my part :)

If i only knew that!

Polymerizing actin creates fireworks that are a sight to behold. This excitable medium produces polymerizing waves similar to those observed in other context. The parallels I tried to lay out in this perspective piece last year: tinyurl.com/53cbxm4v
(LifeAct-GFP in Endothelial Cell)

These are rat fibroblasts after adding PDGF

I thought #fluorescencefriday would be a good opportunity for my first post here. Here are some crazy actin dynamics imaged with TIRF

This dance kills people! Human breast cells with a PI3K mutation showing coordinated cell movement.