Nuestro artículo en JEV, destacado en las noticias de la @uam.es
Este estudio nos posiciona como referentes en el campo de las EVs de plantas. Gran trabajo de Miriam Morales
Gracias @uccuam.bsky.social
www.uam.es/uam/investig...
Huge thanks to the panel members:
Prof. Carmen Montiel
Prof. Fernando Vivanco
Dr. Gloria Alvarez-Llamas
Dr. Milagros Castellanos
Prof. Luis Gandía
Yesterday Ainara González-Moro defended her PhD Thesis brilliantly. The first PhD formed in the ThEVrapies Lab. A great milestone for the group and an honour to have been your director. You have worked hard, learnt lots and have been fundamental for the group in it's rise.
We provide evidence supporting the potential of the following protein families as additional PEV markers: aquaporins, vacuolar-type ATPase complex subunits, fasciclin-like arabinogalactan proteins FLA 10 and FLA13, syntaxins other than PEN1, germin-like proteins and calreticulins
Our data shows consistent specificity for current PEV markers from A. thaliana TET8 and PEN1, but these are not robustly in PEVs from other species. Alternative markers need to be explored, aiming to create a broader set of standard PEV identity markers
Next, we searched for the appearance of protein families from potential plant EV markers in previous proteomic studies of plant EVs. Conservation analyses throughout the plant and animal taxonomy of representative members of these families were also performed
We also analysed protein content of SEC fractions not pooled for isolating EVs and calculated an enrichment degree in EVs. This analysis offered key results to determine marker´s specificity, with some potential EV markers equally present in EVs and the soluble AWF proteome
To accomplish this, we have employed a two-step solubilization method to extract proteins from plant EVs for LC-MS/MS analysis, identifying as many as 1145 proteins in A. thaliana and 899 in B. oleracea, detected in all 3 biological replicates assayed
We achieved the most profound proteomic analysis of AWF-EVs (genuine EVs) from Arabidopsis and red cabbage, ranking potential markers by enrichment in EVs, species-specific abundance and plant specificity, also comparing with previously data and performing conservation analyses
There is urgent need for standardisation of identity markers and evidence of their conservation throughout plant taxa. Hoping this publication helps our efforts in the ISEV Plant Task Force building steps towards rigorous characterisation complying with MISEV.
Major findings👇
NEW PUBLICATION FROM THE GROUP IN #JEV!
A compendium of bona fide reference markers for plant #EVs🌿
isevjournals.onlinelibrary.wiley.com/doi/10.1002/...
🙌 Impressive work by: Miriam M.R. de Lope, Ibone Rubio, @educhicano.bsky.social and more!
@isev.org @snevresearch.bsky.social
Highlights here!👇
Thanks for the invitation!
Inspiring talks and vibrant networking. Great hosting @fafianlabora.bsky.social!
