Brendon Seale

Do you know about machine learning and data visualization? Does someone else you know? Apply those skills and come and join our team as a programmer/data analyst!

Apply here: apply.interfolio.com/178655

Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics - Nature Methods Chip-Tip is a label-free quantification-based single-cell proteomics workflow for deep single-cell proteomics, which identifies over 5,000 proteins and 40,000 peptides in single HeLa cells.

🚨 Our #singlecell article is out! We describe the Chip-Tip label-free SCP workflow quantifying >5000 proteins in single HeLa cells. With high scalability, increased depth and throughput we can now envision large-scale LFQ-SCP biomedical studies!
www.nature.com/articles/s41...

Finally, an experienced MS group performed a proper comparison of MS plasma proteomics vs. Olink! Kudos to the Coon lab! Cannot wait to read all the details!

Boringest study of 2025 so far! 9,000 harmonized QC samples ran by a bunch of labs over 4 years!   In case you're new here or my sarcasm doesn't translate well - this study is amazingly fantastic and probably very very very very boring. ...

News in Proteomics Research blog post | Boringest study of 2025 so far! 9,000 harmonized QC samples ran by a bunch of labs over 4 years! proteomicsnews.blogs...

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#proteomics #prot-other

Not sure of the gradient/flow you are using but at lower flow it can often help to go to a lower ID on the emitter. That's solved a few problems for us, but not as extreme as what you are seeing.

My starter packs:

Proteomics pack (no space left): go.bsky.app/HH7kqEh
ECR proteomics: go.bsky.app/Dp8PHX1
British proteomics: go.bsky.app/S4kiXYL
Single cell proteomics: go.bsky.app/KYdPDb8

IRR-Edinburgh: go.bsky.app/Mca9njQ

Neutrophils: go.bsky.app/34xev7n

I’ve been told they are working on it… since we got ours in January 😕

My proteomics resources on Bluesky:

Proteomics Starter Pack (full): go.bsky.app/HH7kqEh PSP converted into a List so more researchers can be added: (bsky.app/profile/did:...)

Early Career Researchers in Proteomics Pack: go.bsky.app/Dp8PHX1

Single Cell Proteomics Pack: go.bsky.app/KYdPDb8

The NBCC and @gingraslab.bsky.social are hiring a junior programmer/data specialist to help update and advance our proteomics software. If you are motivated and ready to learn, come join our team. For more details and to apply: apply.interfolio.com/147660. #hiring #proteomics

Join us next Tuesday as NBCC's @brendonseale.bsky.social will be presenting at CNPN's virtual meeting highlighting the value of scientific platforms and the vital role they play keeping our facilities operational and at the leading edge.

After a conversation with Bruker application specialist, the consensus looks like this: Bruker PepSep columns thread. @htlau.bsky.social

Super excited to be hosting this event next week with @gingraslab.bsky.social and Sinai Health. Expert talks from @maccoss.bsky.social & Jesper Olsen. Register at t.co/TeuB5bBYl6 and find out why our MS manager @brendonseale.bsky.social loves this instrument.

Hello #TeamMassSpec and BioID fans! We are searching for particular lots of Cytiva Sepharose-Streptavidin resin. Recent lots have been poorly compatible with MS. If you have anything to spare from lots 10326476, 10312459, 10269523 or 10296366 please get in contact!

Even just diluting the provided standards in your lysis buffer can be effective. I did that a few days ago with a 562 nm BCA assay... thought it was Thermo or Pierce, unsure now.

IonOpticks is my go-to for most things these days. And in terms of heater setup for Thermo instruments it's quite affordable.

Nope. It's on the bottom of the stack. It's a fairly compacted design.

That literally happened to us twenty minutes after the techs left!

The HESI “looked better for the photos”

Festivities start early for proteomics researchers at Sinai Health with Canada’s first Astral mass...

Celebrating our new toy. Also the first time I've been quoted in a news release! #TeamMassSpec

There are for sure bugs to work out. Like how 1 in 10 FAIMS runs randomly don't record data.

That's my problem too and I always forget all the syntax etc. when doing so too!

It’s here! It’s running! It still needs to be completely integrated with its crazy server! But it’s here!

Where are your issues lying? It's pretty easy to run it via FragPipe but most of the trouble with these kinds of experiments is in setting up controls etc.

Now this looks like a cool job...

This is true. There is an up-front cost for PaSER. Thankfully no subscription fees!

That’s what I thought… it’s very confusing!

New Astral news: The recommended PC for running ProteomeDiscoverer w/CHIMERYS running from Ardia is almost as powerful as the Ardia server itself... for some reason...

Generally this is done via PaSER/Bruker Proteoscape which works quite seamlessly via the HyStar queue. The PaSER diaPASEF search is based on an earlier version of DIA-NN making them quite comparable.

If Ardia asks me where John Connor is I am going to be very upset...

Purchaser.