Check out this new approach to colocalization analysis from @owfpuls.bsky.social along with members of the AIC and Advanced Bioimaging Unit teams, now out in @jcellsci.bsky.social!
journals.biologists.com/jcs/article/...
🧪 We are excited to share this novel open access paper on Phasor Mixing Coefficient to analyze colocalization, developed by folks @i2janelia.bsky.social and @aicjanelia.bsky.social. This is also the first technical paper jointly published with our sister imaging center @malacridalab.bsky.social!
Looking a little closer with the help of the @aicjanelia.bsky.social we used iPALM to see where septins were in relation to the actin. While they colocalize in the X-Y plane, septins are actually consistently below the actin stress fibers, closer to the membrane.
Starting with your biological question can help focus your #bioimageAnalysis goals. Learn more in the latest @aicjanelia.bsky.social post on @focalplane.bsky.social 👇
In a new post from @aicjanelia.bsky.social and @anjanja1024.bsky.social, Kevin Hamill shares ‘a day in the life’ of an AIC visitor. Kevin’s team went to the AIC to image components of the basal membrane using iPALM.
focalplane.biologists.com/2025/11/24/y...
And, if you've ever wondered what it is like to be a visitor to the AIC, check out this post by @anjanja1024.bsky.social and Kevin Hamill about visiting to use our iPALM system: focalplane.biologists.com/2025/11/24/y...
We are kicking off a blog series with @focalplane.bsky.social to provide insight into life at the AIC, microscopy careers, image analysis, and more! Read our intro post here: focalplane.biologists.com/2025/11/20/a...
We moved the AI@MBL course "Deep Learning for Microscopy Image Analysis" to HHMI Janelia (@hhmijanelia.bsky.social).
Join us for two weeks of intense lectures, exercise, and hands-on project work!
Course dates: June 4-18 2026
Application by: January 15 2026
www.janelia.org/you-janelia/...
I learned so much from the @aicjanelia.bsky.social team at the #InsightFromImaging 2025 workshop this past week. Thank you so much! It was so fun and informative. I enjoyed the discussions between #HannaGrayFellows and the AIC team at @hhmijanelia.bsky.social. I’m off to do bioimaging experiments! 🔬
A photo of a coffee mug. The mug is labeled with writing that says "hhmi | janelia Research Campus." The drink in the mug has the words "Insight From Imaging 2025" printed on the foam.
One last Bob's coffee break at @hhmijanelia.bsky.social before our final #InsightFromImaging activity: Participants are working in groups to formulate a hypothesis and design an imaging experiment to test it 🔬
A 3D rendering of microscopy data. Fluorescently labeled tubulin is shown in green, while second harmonic generation is shown in magenta.
We spent the afternoon at #InsightFromImaging in the lab exploring 5 different microscopes (& 5 different samples!) to understand approaches for imaging depth.
Kidney slice prep by Ellen, imaged by Jenny using 2P (green tubulin) + SHG (magenta), viz by @scientistrachel.bsky.social in #AGAVE
A microscopy image with text annotation. The left side of the image is labeled "System Correction Only" and shows blurry and aberrated mitochondria. The right side of the image is labeled "Adaptive Optics + Deconvolution" and shows a clearer image of mitochondria.
Today at #InsightFromImaging we're focusing on imaging depth, starting with a lecture by @cmhobson.bsky.social. Adaptive optics (available on our MOSAIC system!) can improve deep imaging, as shown by these mitochondria in a zebrafish embryo.
Working with #TrackMate, participants at #InsightFromImaging were able to explore tracking parameters and the tradeoffs inherent in analysis. It was another fun discussion! 🤓
Yes, they are the same field of view 😄 Bonus additional #InsightFromImaging cell from a different FOV here:
That last cell is mostly just floating by, but it is still making protrusions and attempting to engage with the surface and/or the other cells.
This movie was made in @chimerax.ucsf.edu 🙂
HL60 cells take on so many fun shapes as they migrate! This #InsightFromImaging data features cells prepared by Leanna and imaged on the @aicjanelia.bsky.social LLSM by @cmhobson.bsky.social
Continuing our speed theme at #InsightFromImaging, we spent the afternoon at the microscope exploring imaging speed, as well as tradeoffs in imaging scale. Video featuring imaging of neutrophil-like HL60 cells on a widefield vs the AIC's LLSM.
A photo of a classroom. A man stands at the podium next to a projector screen. The audience sits at tables in front of the screen.
Today's theme at #InsightFromImaging 2025 is imaging speed. We're kicking things off with a lecture by Chad Hobson.
A microscopy image of a cell labeled for actin (magenta) and paxillin (green). The left half of the image is taken in widefield mode, while the right half is taken in confocal mode. The scale bar is 10 um.
For our first #InsightFromImaging lab, we're exploring imaging resolution across a variety of microscopy systems. When is increased resolution really necessary? What trade-offs does it require?
I had a wonderful time discussing image analysis with HHMI #HannaGrayFellows this morning. I look forward to continuing the chat throughout #InsightFromImaging this week.
A photo of a man giving a presentation. He is standing in front of a projector screen. In the front of the photo, the audience sits at long tables.
We're kicking off #InsightFromImaging 2025 with a keynote lecture by @scopeshifu.bsky.social on advanced microscopy and the resources available through the Advanced Imaging Center's visitor program 🔬
When #halloween meets #FluorescenceFriday 🎃
Wishing everyone a happy #FluorescenceFriday and a #HappyHalloween! This expansion microscopy visualization of blood vessels in a zebrafish is the result of a collaboration between Ellen, Jenny, @nicolasdenans.bsky.social, and @scientistrachel.bsky.social.
Immunolabeling outlining structures in purple color in a tissue section of human brain at the level of the hippocampus
Hippocampus masquerading as a 🦇 for Halloween 🎃
#FluorescenceFriday
Image credit to the @lieberinstitute.bsky.social Microscopy and Spatial Biology Core 🧠🔬🧪
A 3D rendering of microscopy data. The structure of a fish is visible in gray while green blood vessels are visible through the eye.
When #FluorescenceFriday falls on Halloween, we break out the spooky data 👻 Here we are looking at the blood vessels of a zebrafish through its eye 🎃
Image: three figures in protective suits watch from behind a screen as a child approaches a door-like mechanical structure in which stands a man. Between them is an amorphous glowing shape. Caption: Scientists at the institute of halloween studies have succeeded in creating "The treak": a quantum state that is simultaneously trick and treat. It is stable for a millionth of a second before decaying into a half-hearted prank and a mediocre candy bar.
A halloween cartoon for @newscientist.com
I'm excited to be co-organizing #InsightFromImaging 2025! We have many interactive activities planned, including hands-on image analysis labs 🤓
Today marks 4 years of enjoying the beautiful @hhmijanelia.bsky.social campus 🌳
A photo of two rows of people. The first row of people is sitting on bench while the second row stands behind them, in front of floor to ceiling windows. Outside the windows is a fall scene with trees.
Today we're wrapping up a flatworm imaging project on our SiMView microscope. We've enjoyed having @kinglhung.bsky.social and team from the lab of @ardemp.bskyverified.social here and look forward to working with them on analyzing their images!
