Group Leader Call at the @cbitoulouse.bsky.social in
Genome biology, projects at the interface with devbio will be given particular attention. please share :)
cbi-toulouse.fr/wp-content/u...
Lovely little pre-Christmas present to see this out @natcellbio.nature.com! Some 🔥 new results in here since the biorvix incl (1) a new RARE-GFP reporter ✳️🙌, (2) additional NMP quantification 🔢, (3) no neural tube patterning on RA inhibition 🙅 etc. Enjoy! 😍 www.nature.com/articles/s41...
![NDiff227 gastruloids display a developmental delay in comparison to HM-N2B27 gastruloids. (A) Quantitative analysis of the roundness (perimeter2/4π×area) of gastruloids cultured in either HM-N2B27 or NDiff227 showed that HM-N2B27 gastruloids are significantly less ‘Round’ than their NDiff227 counterparts at 96 h [unpaired two-tailed t-test; t(4)=−4.9541, P=0.008]. Each datapoint is the average roundness of an independent replicate experiment containing between ∼40 and 90 individual gastruloids (see Fig. S4B). (B) The frequency of morphologies from the data in A, with examples of gastruloids that would fall in each category (bottom; the left 'Protrusions' image is also shown in Fig. S4). (C) Light-sheet representative images (maximum projection images) highlighting the expression patterns of Cdh1 (magenta) and Cdh2 (cyan) between HM-N2B27 gastruloids at 96 h and NDiff227 gastruloids at 90 h and 96 h. Gastruloids developed in HM-N2B27 have upregulated Cdh2 by 90 h, unlike NDiff227 gastruloids, which show enhanced Cdh2 only at 96 h. Scale bars: 100 μm. (Ci) Qualitative analysis of the average expression pattern of Cdh2 (N-cadherin). The expression patterns were grouped into two categories (phenotypes I and II) and represent the average of three independent replicate experiments, each containing at least seven individual gastruloids per condition. Examples of the two categories are shown below. The leftmost example for ‘Phenotype I’ is shown in the middle row of 90 h HM-N2B27 (Cdh2) in C. For the box and whisker plot, the central line in the box is the median, the lower edge of the box is the first quartile, the upper edge of the box is the third quartile, and the box height is the IQR. The lower and upper whiskers indicate the smallest datapoint within 1.5xIQR below Q1 or above Q3, respectively.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:btitwyhiegkb6f62oguxt6m6/bafkreiakq7wvgnsnajj2g3bpn5k5p4yu7vznwibobhgzu2o5ttsmjq5ylq@jpeg)
NDiff227 gastruloids display a developmental delay in comparison to HM-N2B27 gastruloids. (A) Quantitative analysis of the roundness (perimeter2/4π×area) of gastruloids cultured in either HM-N2B27 or NDiff227 showed that HM-N2B27 gastruloids are significantly less ‘Round’ than their NDiff227 counterparts at 96 h [unpaired two-tailed t-test; t(4)=−4.9541, P=0.008]. Each datapoint is the average roundness of an independent replicate experiment containing between ∼40 and 90 individual gastruloids (see Fig. S4B). (B) The frequency of morphologies from the data in A, with examples of gastruloids that would fall in each category (bottom; the left 'Protrusions' image is also shown in Fig. S4). (C) Light-sheet representative images (maximum projection images) highlighting the expression patterns of Cdh1 (magenta) and Cdh2 (cyan) between HM-N2B27 gastruloids at 96 h and NDiff227 gastruloids at 90 h and 96 h. Gastruloids developed in HM-N2B27 have upregulated Cdh2 by 90 h, unlike NDiff227 gastruloids, which show enhanced Cdh2 only at 96 h. Scale bars: 100 μm. (Ci) Qualitative analysis of the average expression pattern of Cdh2 (N-cadherin). The expression patterns were grouped into two categories (phenotypes I and II) and represent the average of three independent replicate experiments, each containing at least seven individual gastruloids per condition. Examples of the two categories are shown below. The leftmost example for ‘Phenotype I’ is shown in the middle row of 90 h HM-N2B27 (Cdh2) in C. For the box and whisker plot, the central line in the box is the median, the lower edge of the box is the first quartile, the upper edge of the box is the third quartile, and the box height is the IQR. The lower and upper whiskers indicate the smallest datapoint within 1.5xIQR below Q1 or above Q3, respectively.
The power of the media: core culture conditions direct gastruloid development
This Research Highlight showcases the work from Tina Balayo, André Dias @dias-andre.bsky.social, David Turner @gastruloids.bsky.social and colleagues:
journals.biologists.com/dev/article/...
First author Tina Balayo (left) and corresponding authors André Dias (middle) and David Turner (right)
To find out more about this work and the people behind it, we spoke to first author Tina Balayo, and to co-corresponding authors André Dias @dias-andre.bsky.social and David Turner @gastruloids.bsky.social:
journals.biologists.com/dev/article/...
We would like to thank the reviewers for their inputs, the editor, and @dev-journal.bsky.social for the interview journals.biologists.com/dev/article/... and the highlight journals.biologists.com/dev/article/...
I do like the @dev-journal.bsky.social "Research Report" format for shortish papers
This one is valuable. "Is it the N2B27?" is the stem cell biologist version of "Is it the magnesium?"
Congrats Tina Balayo @dias-andre.bsky.social David Turner & all authors
journals.biologists.com/dev/article/...
Now out in @dev-journal.bsky.social
Lots of new results! Check it out!!
journals.biologists.com/dev/article/...
Now out in @dev-journal.bsky.social
journals.biologists.com/dev/article/...
Now out in @dev-journal.bsky.social
journals.biologists.com/dev/article/...
Happy to present the final version of our Cadherin and gastruloid manuscript, online at @cp-cellreports.bsky.social.
www.cell.com/cell-reports...
Initially in BiorXiv (www.biorxiv.org/content/10.1...) 1/12
🚨JOB ALERT🚨
FRIENDS PLEASE SPREAD and RT
We are building a small expert team within the @bokelab.bsky.social at @crg.eu @prbb.org to investigate fundamental questions in oocyte cell biology, focusing on how proteostasis regulation influences dormancy and fertility.
See below 2 calls👇
THANK YOU for many years of friendship, support and for sharing your great Science
La Pedrera #Barcelona
#NotInTheGenes #CellsRUs #Cambridge
Great opportunity to join the vibrant developmental biology in Toronto (and boss around me and @wbderry.bsky.social).
jobs.sciencecareers.org/job/673968/p...
Catch up on Development presents… July webinar on early embryogenesis
Featuring talks from @lakshmib02.bsky.social, @dias-andre.bsky.social and @nikhil-mishra.bsky.social
🎥 thenode.biologists.com/catch-up-on-...
@dev-journal.bsky.social #DevBio
Tune in tomorrow if you are interested in understanding how stem cells can be used to make different parts of our body
Development presents... Wednesday 9 July 15:00 BST, chaired by Alex Eve. Talk 1: Lakshmi Balasubramaniam (Gurdon Institute) ‘Tissue spreading couples matrix remodelling during avian gastrulation’ Talk 2: André Dias (Universitat Pompeu Fabra) ‘Opposing Nodal and Wnt signalling activities govern the emergence of the mammalian body plan’ Talk 3: Nikhil Mishra (Institute of Science and Technology Austria) ‘Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo’
Sign up to attend our next Development presents... webinar on early #embryogenesis, featuring talks from @lakshmib02.bsky.social, @dias-andre.bsky.social and @nikhil-mishra.bsky.social.
📅Wed 9 July 15:00BST (UTC+1)
Register here: us02web.zoom.us/webinar/regi...
#DevBio @dev-journal.bsky.social
In which we discuss the homologies between amphibian and mammalian organizers from the perspective of #gastruloids with special relevance to recently described A and C gastruloids as containing Spemann/Mangold and Trunk/Tail organizers www.sciencedirect.com/science/arti...
Experimental embryology postdoc available in my lab at the @biology.ox.ac.uk @ox.ac.uk working on the evolution of vertebral counts. Reach out if you’re passionate about EvoDevo, enjoy lab work and microscopy and are into or could get into cichlid fishes. Deadline on the 16th June. Please share!
We've just updated our manuscript to a more 'publishable' version!
www.biorxiv.org/content/10.1...
New details about the role of Wnt in suppressing neural differentiation and fostering a posterior epiblast state, and the temporal effect of Nodal signalling in driving mesendodermal fates
Great work from Alexandra and Sebastian et al showing how gastrulation is governed downstream of Wnt and Nodal. Two competing transcriptional modules - two opposing signalling activities - orchestrate the emergence of the mammalian body plan.
moving forward, together
Difficult to believe that such a community has emerged @ #gastruloids as a model system to explore many aspects of #DevBio. Great to see a room so full of people, curiosity and good Science @prbb.org @upf.edu Thank you to all that have made this possible & of course to the participants
There's increasing evidence that in organoid/embryoids conditions of cells at start of experiments is crucial/ determines outcome. Much to be learnt still. Taking advantage of mouse #gastruloids, two groups explore this here. Important work to calibrate the field www.biorxiv.org/content/10.1...
N2B27 media formulations influence gastruloid development https://www.biorxiv.org/content/10.1101/2025.03.15.643474v1
Fun collaboration! All the nitty-gritty about N2B27 and gastruloids. Kudos to all the authors, particularly Tina Balayo (¡que va de primero!)
🧫Looking for a PhD to start Autumn 2025? We're recruiting! 🧪
The project will focus on using #embryomodels to explore #development & could cover a range of specific questions. Please RT! 🙏
Apply via the portal, and get more details here: www.crick.ac.uk/careers-stud...
The Lanner lab is recruiting a postdoctoral fellow to continue developing our strategy to generate pluripotent stem cell derived photoreceptors for treatment of blindness. Looking for talented colleague to join our team.
@ki.se
ki.varbi.com/what:job/job...
We are looking for a new Executive Editor on the journal, with @katherine-brown.bsky.social taking on a new role at @biologists.bsky.social. If you're an experienced editor with a love of developmental biology and its community, this could be the job for you. Full details in the link below.
I am recruiting an #ERC funded PostDoc to work on the evolution of vertebral counts in cichlids. This is an experimental project & will be looking for candidates with experience generating reporter lines, live-imaging and experimental embryology.
Application deadline: 25th Feb.
tinyurl.com/33jbu8fa
Job Alert!
We are recruiting a postdoc for organoid technology at RIKEN BDR, Japan.
This is a valuable opportunity to work in collaboration with multiple labs, aiming for an advanced integration of developmental biology and engineering.
Please contact!
www.riken.jp/en/careers/r...
