Zoher Gueroui 🦠🔬

FEBS Press ArtiTDP43 allows the control of the assembly and disassembly of cytoplasmic TDP-43 condensates. TDP-43 accumulates into various assemblies, ranging from submicrometric puncta to aggregates that displ...

TDP-43 aggregates are a hallmark of neurodegenerative ALS disease, but how they drive toxicity remains unclear. We present a method to chemically switch cytoplasmic TDP-43 condensates on and off, enabling direct links between aggregation, and toxicity.

febs.onlinelibrary.wiley.com/doi/full/10....

Human monocytes placed on a soft matrix undergo phase separation into micro domains. On glass/plastic, monocytes do not form such domains.

See more details in our recent paper in Nature Materials: www.nature.com/articles/s41...

Organelles harbour pH gradients https://www.biorxiv.org/content/10.64898/2025.12.12.694065v1

Postdoctoral position in the team of Alessandro Barducci in the CBS, focusing on multiscale simulations of RNA-driven condensates, with applications in synthetic biology.

More information :
www.cbs.cnrs.fr/images/jobs/...
The position is expected to start in early 2026, with some flexibility.

FSP1-mediated lipid droplet quality control prevents neutral lipid peroxidation and ferroptosis - Nature Cell Biology Lange et al. identify a lipid droplet quality control pathway in which FSP1 safeguards stored neutral lipids from lipid peroxidation, thereby preventing the induction of ferroptosis.

Excited to share our study out in @natcellbio.nature.com! Led by @mikelangelipid.bsky.social, we identify the first #LipidDroplet lipid quality control pathway: LD-localized FSP1 protects stored lipids from oxidative damage and prevents LD-initiated #ferroptosis.
www.nature.com/articles/s41...

FSP1-mediated lipid droplet quality control prevents neutral lipid peroxidation and ferroptosis - Nature Cell Biology Lange et al. identify a lipid droplet quality control pathway in which FSP1 safeguards stored neutral lipids from lipid peroxidation, thereby preventing the induction of ferroptosis.

Excited to share my postdoc work @olzmannlab.bsky.social! We found lipid droplets, the cell’s lipid storage depots, are subject to oxidative damage and are protected by FSP1. Loss of FSP1 triggers droplet peroxidation and cell death, revealing a new layer of lipid quality control!

shorturl.at/B5XYD

Selective RNA sequestration in biomolecular condensates directs cell fate transitions - Nature Biotechnology Stem cell differentiation is controlled by manipulating RNA condensates.

P-bodies , microRNAs and cell fate ! Not everything is transcription in cell fate decisions ;)

🚀 Our new paper is out @natmethods.nature.com!

Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.

🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/

Wonderful to see our paper on the #organelle signatures of #neurons and #astrocytes out in final form - congratulations, Shannon Rhoads and team!🎉 t.co/BPxKlbU6Ou

Which city is the best for Cytoskeleton research?
Dresden? Barcelona? Cambridge ? Noooo ... you all know its Paris, right? 😎
Registering to the annual meeting on Friday 14th of November is now open:
pcd2025.sciencesconf.org
Keynote speeches by @carter-lab.bsky.social and Verena Ruprecht !

Big Thanks @lisaheinke.bsky.social and Nature Reviews Molecular Cell Biology @natrevmcb.nature.com for the opportunity for our students, Chems and Emma, to write a Tools of the Trade piece on our recently published ControLD technique.

Do you know what happens if you squeeze a #cell?

If the #confinement is high, the nucleus (green) will be super tense & the compressed cells will react like this👇🏽changing their #cytoskeleton (magenta)

Full: www.science.org/doi/10.1126/...
#scicomm #microscopy #sciencesky @focalplane.bsky.social 🧪🔬

The 2025 @cnrs.fr medal are out! Bronze medals for early career researchers: congrats to Aurélie Tchoghandjian at our institute @inp-marseille.bsky.social, and to @alexandra-colin.bsky.social who was nominated by the Cell biology section 🎉

Controlling lipid droplet dynamics via tether condensates - Nature Chemical Biology Amari et al. developed a condensate-based approach, named ControLD, for reversible trapping and release of lipid droplets inside living cells, temporarily halting their functions, enabling detailed st...

A condensate-based approach reversibly sequesters lipid droplets (LDs) in cells, temporarily halting their functions and enabling detailed study of LD biology and the interactions of LDs with other cellular structures

www.nature.com/articles/s41...

Thanks Benoit!

Thanks Ebony!

What if we could trap Lipid Droplets and control their dynamics in cells?
Our latest work does just that, using Engineered Condensates to manipulate LD behavior.
Exciting results by Chems Amari and the team!
Great collab with ‪@abdourachidthiam.bsky.social‬
#Organelles #Condensates

rdcu.be/emYpU

Small-molecule dissolution of stress granules by redox modulation benefits ALS models - Nature Chemical Biology Uechi et al. found that a small-molecule lipoamide dissolves stress granules (SGs) by targeting SFPQ, a redox-sensitive disordered SG protein, alleviating pathological phenotypes caused by amyotrophic...

Hiro's paper is out! @hiro-cg5445.bsky.social
Collaboration and Tour de Force with Richard Wheeler @zephyris-science.bsky.social and many other coauthors.
Big congratulations and thank you to anyone involved!

www.nature.com/articles/s41...

Lipid-degrading small molecule kills cancer cells by ferroptosis A molecule designed to activate iron locked up in organelles called lysosomes and thereby induce cell death might offer a way to tackle treatment-resistant cancer.

Excited to see our N&V w/ @mikelangelipid.bsky.social published in Nature, highlighting new work from Raphael Rodriguez and team. A heterofunctional molecule that activates lysosomal iron triggers lipid peroxidation and ferroptosis in therapy-resistant cancer cells. www.nature.com/articles/d41...

Oligo-LiveFISH enables high-res, live imaging of non-repetitive DNA loci in 3D across cell types, overcoming past limitations in dynamic genome studies. PMID:40239646, Cell 2025, @Cell https://www.cell.com/cell/fulltext/S0092-8674(25)00350-2 #Medsky #Pharmsky #RNA #ASHG #ESHG 🧪

I am excited to share the latest paper from my lab where we leverage the selection bias of condensate-promoting oncofusions to uncover molecular rules governing condensate specificity and function. www.cell.com/cell/fulltex....

How can a cell control the size of its organelles?

Excited to share a mechanism to control droplet size we recently found.

a 🧵

www.nature.com/articles/s41...

We’re excited to share our work where we investigated the mechanisms of chromosome motion by tracking all #chromosomes, mapping their interactions, and live cell karyotyping, using #AI based denoising, segmentation and registration, published in Nature Cell Biology.
www.nature.com/articles/s41...

EPSILON: a method for pulse-chase labeling to probe synaptic AMPAR exocytosis during memory formation - Nature Neuroscience Kim and coworkers describe a technique, EPSILON, to map AMPA receptor exocytosis, a proxy for synaptic plasticity, in mice. The authors demonstrated a correlation between AMPA receptor exocytosis and ...

Which synapses get stronger when we form a memory? We developed a technique to tag recently potentiated synapses in live mice. Pulse-chase labeling with membrane-impermeable HaloLigand dyes distinguishes fresh AMPA receptors from old ones. www.nature.com/articles/s41...

A tunable and versatile chemogenetic near-infrared fluorescent reporter - Nature Communications This paper reports the engineering of nirFAST, a near-infrared fluorescent chemogenetic reporter suitable for multi-color protein imaging in cells and organisms, as well as for the design of cellular ...

Very happy to share the recent paper of Lina El Hajji in Arnaud
Gautier's team on the development of a tunable chemogenetic
near-infrared fluorescent reporter for imaging proteins in live cells and organisms
www.nature.com/articles/s41...

Immiscible proteins compete for RNA binding to order condensate layers Biomolecular condensates mediate diverse and essential cellular functions by compartmentalizing biochemical pathways. Many condensates have internal subdomains with distinct compositional identities. ...

Excited to share our first story on nuclear paraspeckles! Led by @sneadlab.bsky.social, this is the culmination of a ~4 year effort in collab. with @shrinivaslab.bsky.social. Can’t wait to see more paraspeckle stories from the future Snead Lab at Northwestern 🫧 www.biorxiv.org/content/10.1...

Axonal RNA localization is essential for long-term memory - Nature Communications Axonal transcriptomes have been characterized, but their regulation and role in neuronal circuits remain unclear. Here authors uncover activity-dependent RNA localization in axons important for memory...

Axonal RNA localization is essential for long-term memory
www.nature.com/articles/s41...

Intrinsic hydrophobicity of IDP-based biomolecular condensates drives their partial drying on membrane surfaces The localization of biomolecular condensates to intracellular membrane surfaces has emerged as an important feature of sub-cellular organization. In this work,

New paper out! Why do condensates prefer some surfaces and not others? Check it out here: doi.org/10.1063/5.02...

@science.org Cryo-EM reveals mechanisms of natural RNA multivalency | Science www.science.org/doi/10.1126/...
#RNAsky #RNAbiology #Cryo-EM

A small but special moment: My illustration is on the cover of the March issue of Nature Methods!

A huge hug to @anwaiarchit.bsky.social and @cppape.bsky.social — it was a pleasure to work alongside you on this!

www.nature.com/nmeth/volume...