Dorothy Koveal

Second preprint from the lab. Collab with @dkoveal.bsky.social, with many more to come! Effort led by @xshirleyz.bsky.social with help from Brittany Addison, @ezeyulu00.bsky.social, Claire Deng (on the grad school market, better act fast, Claire’s amazing!), @ajemanuel.bsky.social, and many others!

High-resolution in vivo kinematic tracking with customized injectable fluorescent nanoparticles Injectable fluorescent nanoparticles were used to track positions on and inside of freely moving animals at high resolution.

Our first preprint has been accepted for publication www.science.org/doi/10.1126/... !!
tldr: @ezeyulu00.bsky.social , @amartyapradhan.bsky.social , @dkoveal.bsky.social and I developed a method using injectable nanoparticles to turn mice into…constellations in motion. 🧵⤵️

This work was driven by the singular vision and dedication of Zeynep Ulutas, @ezeyulu00.bsky.social . We could not have finished this without @amartyapradhan.bsky.social . @dkoveal.bsky.social provided key guidance on chemistry.

a man in a suit and tie is dancing in a room in front of a wall . ALT: a man in a suit and tie is dancing in a room in front of a wall .

Beautiful structural analysis of a lifetime sensor from Rosen et al. Really cool mechanism reveal - something I never would have guessed when we made LiLac. A must-read for sensor folks!

www.pnas.org/doi/10.1073/...

Mechanism of giant magnetic field effect in fluorescence of mScarlet3, a red fluorescent protein Several fluorescent proteins, when expressed in E. coli, are sensitive to weak magnetic fields1. We found that mScarlet3 fluorescence in E. coli reversibly decreased by 21% in the presence of a 60 mT ...

How do tiny magnetic fields affect biochemistry? Overflowing with pride over Katherine Xiang's study on a giant magnetic field effect in a red fluorescent protein, www.biorxiv.org/content/10.1...

Could one envision a synthetic receptor technology that is fully programmable, able to detect diverse extracellular antigens – both soluble and cell-attached – and convert that recognition into a wide range of intracellular responses, from gene expression and real-time fluorescence to modulation..

Figure 1. Screening and characterization of glucose sensors. A) The screening assay identifies responsive glucose sensors in a medium-throughput manner. Plates lacking glucose have YSH757 yeast cells expressing sensor variants and are visualized whereafter glucose is sprayed on the plate and visualized again. Dividing the 2 images yields a ratiometric image showing which colonies have a responsive sensor. Colour indicates ratio of fluorescence after the spray divided by the fluorescence before the spray. B) normalized change of each colony tested on the plate, colour indicates basal fluorescence. C) design of the best performing variant. D) In-vivo CFP emission spectrum of YSH757 cells expressing TINGL. Colour indicates amount of glucose added to the cells. Data obtained in a fluorescence plate reader E) In-vivo Dose-response curve of YSH757 cells expressing TINGL or TINGL-RR obtained using a plate-reader. Fluorescence and CFP:RFP ratio are in absolute values. Line shows a Hillfit through the data points. F) Dose-response curve of TINGL-RR of cell-free extracts. Top facet shows CFP:RFP ratio versus mM glucose with the shapes depicting the pH. Line shows a Hillfit through the data of pH 7.5, 7 and 6.5 combined. Colored panel indicates the physiological pH of yeast.

Great to see this online as #preprint:
TINGL - A Turquoise INdicator for GLucose by the lab of @basteusink.bsky.social (and a small contribution from me): www.biorxiv.org/content/10.1...

A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic events Hao, Lee, et al. report ASAP5, a GEVI that enhances responsivity and accelerates kinetics. It enables single-trial recordings of sub- and suprathreshold activities in flies, mice, and human stem-cell-...

Out yesterday in Neuron, the final print version of our paper on voltage imaging with ASAP5, "A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic events"

www.cell.com/neuron/fullt...