Our new preprint is out＠bioRxiv: www.biorxiv.org/content/10.1...
@masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/

Red and blue poster supporting Stand Up for Science event 3-7 at Seattle Center noon to 3 includes a blue statue of liberty, and more information and a QR code. standupforscience2025.org

Among speakers Fri in #seattle,
brain surgeon Jim Olson, MD.
Who helped develop a "tumor paint" from scorpion 🦂
venom with @fredhutch.bsky.social
And @meadekrosby.bsky.social Climate scientist 🌏
Watch thread for more speakers @sufsseattle.bsky.social
🧵

One of the many, many “pauses” and cancellations devastating our next generation of researchers. “The NIH Intramural Program has paused the recruitment of IRTAs, CRTAs, and Visiting Fellows in all training programs pending guidance from Health and Human Services. Check back daily for updates.”

Chromatin conformation, gene transcription, and nucleosome remodeling as an emergent system Gene transcription is an emergent phenomenon of packing domain geometry in situ.

In effect, our genomes are a very sophisticated computer system. We take measurements of the state (salts, food, stressors), integrate it with our prior measurements (chromatin remodeling from prior exposures, epigenetic memories of our grandparents) and adapt.
www.science.org/doi/10.1126/...

Let's start a conversation about this. I keep thinking about how what we do now spans across generations. #Epigenetics and #chromatin is a system for our genomes to tell our cells what to expect in the future and to prepare our children/grandchildren for current-ish conditions.

PREPRINT! Park et al. makes the case that we may be misunderstanding heterochromatin for past 30 years due to ChIP-Seq biases... 1/n

Frontiers | Inclusive mentorship of pediatric trainees: pediatric oncology as a microcosm

Very pleased - on this week of all weeks - to have this paper on the importance of inclusivity in mentorship published. Co-authored with the fantastic Drs. Sadhana Jackson and Jessica Tsai (@jestsai.bsky.social), we do a deep dive on pediatric oncology, but 1/3

www.frontiersin.org/journals/onc...

Nuclear blebs are associated with destabilized chromatin packing domains Disrupted nuclear shape is associated with multiple pathological processes including premature aging disorders, cancer-relevant chromosomal rearrangements, and DNA damage. Nuclear blebs (i.e., herniat...

Our new manuscript up now! journals.biologists.com/jcs/article/... Nuclear blebs are hallmarks of cancer and aging. The 3-D structure of #chromatin domains inside blebs is completely deformed.

We hypothesized that this unique cohesin-mediated structural change in nascent chromatin domains may be caused by cohesin activity on the surface of mature chromatin domains. Using SMLM to image RAD21 and EDU-labeled chromatin, we found RAD21 primarily localized in DNA-poor areas between domains.

Mature chromatin packing domains persist after RAD21 depletion in 3D RAD21 contributes to nascent packing domain formation, but maturation requires nucleosome posttranslational modifications.

CPGE's most recent publication on cohesin function and chromatin domain organization is out now in Science Advances. This thread will take a look at some of the key observations from the paper!

#chromatin #genomics #epigenetics #3Dgenome #northwestern #dna #cpge

www.science.org/doi/10.1126/...

Our paper on cohesin regulation of mesoscale chromatin organization is out

Chromatin conformation, gene transcription, and nucleosome remodeling as an emergent system Gene transcription is an emergent phenomenon of packing domain geometry in situ.

There’s a lot to unpack in our recent Science Advances paper - come check it out! Here’s a thread on what we found.

#chromatin #genomics #biophotonics #dna #epigenetics #imaging #northwestern #science #biology #aging #longevity #biomedicine #cpge

www.science.org/doi/10.1126/...

mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription Transcription inhibitors also disrupt nuclear export. Here, Williams et al. reveal that mRNA export factors sense transcription inhibition and adapt by storing mature export-competent mRNA in nuclear speckles. This enables rapid release when transcription resumes and ensures retention of cellular identity and viability during a transient global transcription insult.

mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription
#RNAbiology #RNAlocalization

www.cell.com/molecular-ce...

Our latest paper on chromatin domains and genome function is out!

Hi Bluesky! I made a 3D chromatin starter pack.
Let me know if you would like to be added.
go.bsky.app/6tTQdqQ

mRNA expression is co-regulated by non-nucleolar RNA polymerase I The relationship between gene transcription and chromatin organization is an area of active study. Due to its role in mRNA synthesis, many studies have focused on the regulaton of RNA polymerase II (P...

Catch the preprint here:

www.biorxiv.org/content/10.1...

Out here at the #4DNucleome meeting in San Diego with a poster on our recent preprint where we explore the impact of Pol 1 and Pol 2 transcriptional inhibition on mesoscale chromatin organization.

#3DGenome

Center for Physical Genomics and Engineering The mission of the Center for Physical Genomics and Engineering at Northwestern University's McCormick School of Engineering is to create new strategies for the treatment of disease and the reversible...

CPGE's archive of recorded talks can be viewed on our YouTube channel at www.youtube.com/@PhysGenCenter.

Visit us at www.physicalgenomics.northwestern.edu for details on our transdisciplinary research platform, training programs, and initiatives such as the Monthly Seminar on Physical Genomics!

Single-molecule states link transcription factor binding to gene expression - Nature A study uses single-molecule footprinting to measure protein occupancy at regulatory elements on individual molecules in human cells and describes how different properties of transcription factor bind...

Single-molecule states link transcription factor binding to gene expression www.nature.com/articles/s41...