💸 Stem cell culture that’s ~95% cheaper and weekend-free: a win-win for PIs and trainees!
Our revised manuscript is online at ORE: open-research-europe.ec.europa.eu/articles/4-1...
Benchmarked across cell lines & differentiation protocols, including scRNA-seq — with lessons for #cultivatedmeat too!
Our review, "Revitalizing the heart: strategies and tools for cardiomyocyte regeneration post-myocardial infarction." doi.org/10.1038/s415..., has just been published in npj Regenerative Medicine!
Created a second stem cell biology starter pack, as the first one was full.
Let me know if you would like to be added!
go.bsky.app/UG3eYwU
Cardiac scratch wound assay to show the role of ECM in wound healing. HiPSC Cardiomyocytes in white on the boarders with a rich ECM scar tissues (cyan) through the middle with a simple nuclear stain (red) highlighting all cells.
How to fix a broken #heart 💔->❤️
#Proteoglycan rich #ECMatrix scar tissue is laid by migratory cells after a cardiac scratch wound with #hiPSC #Cardiomyocytes.
Happy #Fluorescencefriday #BlueSky & #CardioSky!
@ablanchasensio.bsky.social 👀
#Science 💥🧪 #Microscopy 🔬
With Christmas just around the corner, it’s time for the #FluorescenceFriday post🎄!
This week, we’re featuring a hiPSC reporter line engineered to express 2 fluorescent proteins—located in the nuclei and actin filaments of #hiPSCs, generated using STRAIGHT-IN Dual🧪🔬
@benjaminbjohnson.bsky.social?
There are so many #morphogenesis researchers here!
My first starter pack is full!
Here is Part 2: go.bsky.app/K6YYKeC
#devbio #cellbio #science
Tell me if I should add you but try to make sure, that you're not already in Part 1.
I cannot cross-reference everyone 😅
Part 1: go.bsky.app/RzDD9or
New starter pack for all people interested in Gene Editing & Gene Therapy 🧬✂️ including Tools & Delivery|CRISPR-Cas|Zinc finger nucleases|Viral vectors|Non-viral delivery methods
go.bsky.app/8R4dVez
Can I be added here, please? Thanks!
Created a starter pack focusing on disease modeling in a dish using stem cells! 🧪🔬
Please (self)nominate anyone you think should be added to the list and repost so it finds the right people ❤️
hPSC Disease Modeling: go.bsky.app/EpWsxNG
For a good general stem cell pack go here: go.bsky.app/UTW4d7b
Can I also be added please? Thanks!
Working on a #StarterPack to collect researchers using human biology-based tools ( #organoids, #microphysiologicalsystems, other human-derived #invitro tools) + computational models & the orgs & accounts that promote non-animal science. Small so far, but I'm new here. Msg to add.
go.bsky.app/SVWGwgu
Hi! Can I be added please? Gracias!
Cyan cellular extracellular matrix fibers are highlighted laying over the top of the orange hyaluronan secreted matrix after a scratch would assay.
Dedicating this #Fluorescencefriday to the #ECMatrix lovers. Was trialing some ECM staining methods on a scratch wound and love the HABP staining looking almost like flames 🔥🧪🔬
Lets see what you got, @ablanchasensio.bsky.social 😉
Can I also be added please? Thanks!
Added!
Same, but in 3D cardiac organoids 🚦🔴
Calcium signals in beating hiPSCs-derived cardiomyocytes.
A single copy of this reporter was integrated using the STRAIGHT-IN platform at the AAVS1 locus.
doi.org/10.1016/j.cr...
doi.org/10.1101/2024...
Genetically-modified hiPSCs expressing the genetically-encoded calcium indicator jRCaMP1b with a nuclear export signal (NES). This reporter is used to track calcium dynamics in live cells. It combines a red fluorescent protein with calmodulin and a calcium-binding domain, changing fluorescence intensity upon calcium binding. Its red-shifted emission is ideal for imaging in complex tissues or dual-color experiments, making it valuable for studying calcium-mediated processes. A single copy of this reporter was integrated using the STRAIGHT-IN platform at the AAVS1 locus. See below! https://doi.org/10.1016/j.crmeth.2022.100300 https://doi.org/10.1101/2024.10.17.616637
Here is my 2nd #FluorescenceFriday post! 🧪 #hiPSCs expressing jRCaMP1b, a genetically-encoded Ca2+ indicator 👻. This reporter allows us to record intracellular Ca2+ in hiPSC-derived #cardiomyocytes and cardioids (videos in the comments) #microscopy 🔬 #Science #Biology @benjaminbjohnson.bsky.social
My colleague @benjaminbjohnson.bsky.social has just created a starter pack for hPSC disease modeling. Check it out! 🧪🧫
🧪
Graphical abstract depicts automated setup to generate 3D cardiac mini tissues consisting of 3 cell types seeded into 384 well plates. The cardiac tissues are monitored using calcium traces to detect arrhythmias and subsequent rescue after compound addition.
🔥Super excited to drop our recent preprint🔥
biorxiv.org/cgi/content/...
Here, we automated the generation of 3D Cardiac Microtissues and performed a small high-throughput screen to identify several novel anti-arrhythmic compounds for CPVT1 🧪❤️
#Cardiosky #Science #hiPSC #Arrhythmia #Stemcell
We designed MS2 and PP7 binding coat proteins that are degraded in cells except when bound by their hairpin RNAs.
You can check out our pre-print here: tinyurl.com/27p7hyt9,
and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...
that is right!
Hey #BlueSky! Here is my first #FluorescenceFriday post!
This cardiac organoid was derived from #hiPSC and imaged using light sheet #microscopy 🔬.
🔵 Cardiomyocytes
🔴 Endothelial cells
🟡 Fibroblasts
More coming next week! 🧪
#Science #Biology #CellBiology #SciArt
@benjaminbjohnson.bsky.social
Still a few spots left in the Mammalian Synthetic Biology starter pack!🧪
I thought it would be a good idea to create a starter pack for Engineered Living Materials so the community can grow and post in this platform.
Please share and feel free to contact me or comment if you want to be added (or removed)!
go.bsky.app/EKRq4zp
Added! Welcome Alex!
Can I be added please? Thanks!
Added!
Yes ofc, added!
