Witold Szymański

SLS, SDC? You can precipitate them out in low pH.

#Autism causes #Vaccines #Humour #ScienceHumour #MAHAha #LunaticRFKJr

@openvibe.social quite often, in light theme, I don't see my clock and other controls on top as the font is white on white background. But then sometimes I see it as the text becomes gray. Is it a bug from open vibe or android stuff?

Ms upgrade!!! +10% to IDs

There is something poetic in this email 🫠

Yes, exactly.

Single-pot, solid-phase-enhanced sample preparation for proteomics experiments - PubMed A critical step in proteomics analysis is the optimal extraction and processing of protein material to ensure the highest sensitivity in downstream detection. Achieving this requires a sample-handling technology that exhibits unbiased protein manipulation, flexibility in reagent use, and virtually l …

Wait... What? I think we talk about different SP3 :) why expensive? Are you thinking about this? pubmed.ncbi.nlm.nih.gov/30464214/

Yes, that what I meant, with the "dirty" and "clean" samples. But the method of EV prep also affects the content in the sample, of the EV proteins, apart of the background.

We use SP3 for the sample prep of EVs. We tested it against C18 many times and it was way better. We can wash away any detergent or salt and there is no carryover or effect on chromatography.

But it's also a question to EV biologist, if they really expect to have >2000 proteins in their samples.

We commonly use higher % of SDS for the EV lysis, bit now I'd like to test the SDC :)

So many variables at play.

@evcorefacilty.bsky.social @chpreusser.bsky.social

Nice and easy paper. However, the EV protein content is massively dependent on the isolation method. If I understood correctly, they "just" used UC, which is the "dirtiest" method. I wander how this whole comparison would look like with different EV preps: TFF, SEC, UF, combination of those?

Why not? They didn't want to come?

RIP... :(

How did I not think of this ?

So insightful.
So specific.

Just leverage AI.

(given the information density, multiple readings may help)

UK? 😏 Or does it still count as Europe?

How does it compare to Quarto? Worth a switch?

@kerrynugent51.bsky.social 's magic column continues to live. These Hela and BSA-QC runs are 1100 injections apart, running mainly with one-column methods on nanoElute2 from #bruker 🥳

Starting today, only certified Evosep engineers will show up in the official orange cape and mask.
Make sure your service hero is properly attired before letting them near your system!
#proteomics #lcms

What about that 24+k emails?! 🫠🤣

A simplified perchloric acid workflow with neutralization (PCA-N) for democratizing deep plasma proteomics at population scale Large scale plasma proteomics studies offer tremendous potential for biomarker discovery but face significant challenges in balancing analytical depth, throughput and cost effectiveness. We present an...

Our PCA-N workflow featuring a neutralization step allows direct enzymatic digestion, doubles proteome depth from 5µL neat plasma, and handles 10K+ sample-preps daily. Democratizing plasma proteomics with 50K sample validation. Check it out on bioRxiv!
www.biorxiv.org/content/10.1...

I already ordered all the missing chemicals 🫠

Do you actually need to read and understand all this stuff? 😱😵‍💫🫠

The "save scroll location" is a saviour!! I really don't understand, why the official app of @bsky.app can't do that.

Merica!

Can DiaNN in CLI on Linux produce the QC plots now? Somehow I never managed to get it work.

Thanks a lot!

For no particular reason at all, I want to give a shout-out to @signal.org - do yourself and your data privacy a favor and switch over from other messengers (tell your friends too!)

Only way your data could be compromised there is if you accidentally invite a journalist to your group chat 👀

https://youtube.com/shorts/RnyUlMS5RAA?si=E3WNT-3aifshHkZn This race was a blast! Best Milan San Remo that I have watched. 🎉 And the commentary was 11/10💪 Rob does it best.

Huh! Idk, I have to call all my local representatives :) now I have many options to try out. Thanks all!

It would actually be interesting to compare all of them... Hmm...