Kelvin Lau @klausenhauser on 🙅‍♂️🐘🪡

Knowing the kinetics from their previous experiments also got me thinking of some interesting questions

Not my sample. Was a student at the course here. That is my assumption. Or some “release” either of water or protein. I’m imagining a weakly self associated helix this gets pushed off by a binder… that also doesn’t form many bonds.

How much about thermodynamics of AI binders are known? I think this is the first time I’ve seen an endothermic interaction. (Kinetics is fast on fast off) #EMBLProteinQC

Currently it is still in essence getting cut. No increases for inflation just means in real amounts it’s just a smaller budget

In our lab we want the dna and rna gone so it’s great!

Indeed!! More fun, faster and cheaper!

Happy that when public services were put to a referendum (proposal to decrease of the annual household charge of 335 CHF (~$430) to 200 for the public broadcaster) that people understood and made the right choice.

the minipreps of the plasmid itself sometimes get degraded overtime due to residual protein!

Started this project years ago, but now everything is deposited on @addgene.bsky.social and a short @protocolsio.bsky.social is online. Try it out to make your own (cheap) homemade nuclease/Benzonase!

dx.doi.org/10.17504/protocols.io.261ged7xov47/v2

Thank you @jcoker10.bsky.social and Michael Lim!

Coptic Church

Inscription that says “the well which the holy family drank from”

Synagogue

Old Islamic period home

Those were some Pharonic and Islamic places of interests. Lots of Beautiful Coptic ones as well and some rare Synagogues

Inside the new Grand Egyptian Museum

View of the pyramids

Cat in Mosque

Ramasseum

First vacation that I spent more on museums and temples than on food. What a rich cultural history Egypt has spanning millennia

It’s the first time I can actually be honest in my message :)

I wish! I would be getting blasted in any game now

Oh that’s a good one!

Its that time of the year again OOO

New BLM Bergman in front of the mountains, Mönch and Jungfrau

A view of Eiger, Mönch and Jungfrau

Interior of the mid-mountain Birg station

Ski slopes with Schilthorn in the distance

My favourite place this weekend. And some new infrastructure sightings. New train and a new gondola mid level station (nicer than some airports!)

Nope it’s a Ni-IDA like from Cube Biotech. Blue Sepharose is really really Blue like Indigo

Oops *resin!

What’s your end of the year task? Cleaning all the reason we use in half a year. Of course on an indestructible Amersham era pump with its detector intact

Seen elsewhere… and was triggered. Don’t think I would set up the void peak that has the lower melting temperature if I only had one chance. The small monomer and higher melting curve in the dotted example however.. don’t trust your AI infographics

Saw this nice short protocol paper on LI. Not totally HTP but totally feasible in an academic lab. Right in the intersection of drug discovery, automation, structural biology and protein expression. Ps the guys looking for a job downunder!

www.biorxiv.org/content/10.6...

Nipah Binder Competition Design a protein capable of neutralizing the Nipah virus, a pathogen with up to 75% mortality rate and high pandemic potential, currently lacking effective treatments.

Most protein design binders look the same, bundles of alpha helices or clearly a VHH. But the strategies in getting there are getting more diverse. The pipelines that humans are deciding to use are super interesting. proteinbase.com/competitions...

Lots of fun working on this project! Looking forward to see more and more animations published in the future!

From a pathogenic bacteria…. Of flies

Cryo-EM reveals open and closed Asgard chromatin assemblies Ranawat et al. show the cryo-EM structures of Asgard archaeal chromatin assemblies, revealing that the histone HHoB assembles into both compact closed and extended open hypernucleosomes. The closed co...

Let’s wrap things up: my commentary on the Asgard hypernucleosomes.

Congratulations to all the authors of the paper 🍾!

kwnsfk27.r.eu-west-1.awstrack.me/L0/https:%2F...

Cryo-EM reveals open and closed Asgard chromatin assemblies: Molecular Cell www.cell.com/molecular-ce...

Interesting SEC as well. Hint: putative lectin binder.

looking back at things to delete. I loved this protein. It makes for fun questions to ask for undergrads.

so good 🥮

always exceptions. but Im still not happy!

#1 lesson I give to protein designers for wet lab purposes : Please design with a Trp and/or multiple Tyr. Please do not design with 10 cysteines.