Misha Kudryashev

haha, yes, exactly! I had to post it :)

Yes, exactly!!!

Wish everyone a New Year resolution of 2-3 Ångström!!!

We love isonet and isonet2!!

❄️🔬⏱️
I am excited to share that our study „Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons” has been published in Nature Communications www.nature.com/articles/s41...

👇 Short summary of our key findings (1/8)

yeah, exactly!!!

Academia is just a job - Nature Human Behaviour Working in academia can be stressful. Laurel Raffington suggests treating it as ‘just a job’ to reduce performance pressure and advocate for structural improvements.

Well, that's one way to look at it. Although I am sure that people with dedication would also take a coffee shop job way too seriously and will be amazing in it!

Academia is just a job | Nature Human Behaviour share.google/7IQ3wak0pmOS...

Postdoctoral Positions in Protein Engineering Post a job in 3min, or find thousands of job offers like this one at jobRxiv!

We got a large Helmholtz Bioengineering grant with Oliver Daumke, Artur Yakimovich, Alina Bazarova, and Dietrich Ruess to use protein design to target cancer.

Five open postdoctoral positions in Generative AI & Protein Engineering. Share & apply!

jobrxiv.org/job/max-delb...

Beautiful, congratulations! to you and all the Dutch cryo-EM and scientific community!

We were very happy to help, in a very small way, in getting this beautiful structure out. The structure is remarkably conserved considering the famous 28S ribosomal RNA split. I suspect that the split has as yet unknown biological repercussions in this remarkable animal..

Structures of Naked Mole-Rat, Tuco-Tuco, and Guinea Pig Ribosomes–Is rRNA Fragmentation Linked to Translational Fidelity? Ribosomes are central to protein synthesis in all organisms. Among mammals, the ribosome functional core is highly conserved. Remarkably, two rodent species, the naked mole-rat (NMR) and tuco-tuco dis...

Related reading:
1. A recent preprint by Cristing Gutterrez-Vargas et al with several structures of rodent ribosomes, including the naked mole rat, biorxiv.org/content/10.1...
2. The classical 2013 paper with functional characterization of the NMR ribosome: pmc.ncbi.nlm.nih.gov/articles/PMC...

No spoilers this time, just the beautiful structure!

Mehmet Gul led the work with the dream team of Alice Rossi, Christian Spahn, and Gary Lewin.

Enjoy it and have a fantastic day

Cryo-EM structure of the naked mole-rat ribosome reveals a stabilized split 28S rRNA The naked mole-rat (Heterocephalus glaber) is a long-lived mammal with remarkable resistance to cancer and hypoxia, suggesting the evolution of robust proteostasis networks. The ribosome, the central ...

We have a new preprint for you. Naked mole rats are very long-lived rodents and have increased translational fidelity.

Interestingly, 28S ribosomal RNA is split. Here we investigated the structure of the ribosome by single particle cryo-EM. Take a look.
www.biorxiv.org/content/10.1...

Group Leader in Biomedical Engineering at the @mdc-berlin.bsky.social in Berlin.

It's a broad call for whoever is doing exciting Bioengineering, please apply or share. We have a great institute with amazing colleagues and core facilities,highly recommend

application.mdc-berlin.de/site/index.p...

It's a team effort of the group: Igor, Ulja, Xiaofeng, Artsemi, Patricia, and we thank the funders.

The print version will come up in a book on methods in molecular biology, hopefully later this year!

Enjoy reading and have a great week!

But for a larger system, smaller proteins, interactions with the cytoskeleton, cluster formation, and other biological applications, there seems to be a use of this microvesicle technique.

Membrane protein isolation and structure determination in cell-derived membrane vesicles - PubMed Integral membrane protein structure determination traditionally requires extraction from cell membranes using detergents or polymers. Here, we describe the isolation and structure determination of pro...

Some thoughts: wonderful papers by Roderick Mackinon and other groups show that 2D imaging of membrane proteins in small vesicles can result in high-resolution structures. So if you want structures - try that!

pubmed.ncbi.nlm.nih.gov/37098056/

The book chapter is a set of methods to express your membrane protein of interest, purify vesicles, image them by cryo-ET, and perform subtomogram averaging.

As usual, we like to go into details:

The chapter is inspired by our recent paper, where we show that tetrameric and pentameric forms of the serotonin receptor ion channel 5-HT3R were present in the same membrane patches
www.embopress.org/doi/full/10....

We have a preprint of a book chapter for you: Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles.

We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography

zenodo.org/records/1717...

Roses are red
Because they have SCREP!

Lithium deficiency and the onset of Alzheimer’s disease - Nature Lithium has an essential role in the brain and is deficient early in Alzheimer’s disease, which can be recapitulated in mice and treated with a novel lithium salt that restores the physiological level...

I'd say it's a paper of the week if not a year

Lithium deficiency and the onset of Alzheimer’s disease
www.nature.com/articles/s41...

Postdoc program at the MDC in Berlin, @mdc-berlin.bsky.social : take a look 👇👇

For this call, collaborative projects between two groups will be funded. Take a look at the call and the groups and drop me an email if interested. Share with colleagues too!

www.mdc-berlin.de/postdocs#t-g...

Building atomic models to cryo-EM maps of huge molecules is intimidating: crowded, uncertain copy numbers, variable resolution ...
Fan Liu and team incl us, show how cross-linking MS can be used to build novel models of unknown complexes, like a giant Melbourne virus
biorxiv.org/content/10.1...

Thanks Mimi! ❄️❄️❄️

Thanks Sandip!

Thanks John !!!

We have a preprint for you

EHD2 forms rings on caveolae necks, in contrast to most EHDs forming helices. We determined its structure on membranes and show that N-term acts as a spacer

By Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff &Oli Daumke www.biorxiv.org/content/10.1...

It's fellowship season in the EU. If you want to talk and join our group at the amazing MDC in Berlin on a fellowship, please write me an email.

We do cryo-EM/ET, membrane proteins, data science/AI, our work: scholar.google.com/citations?so...

Fellowships: Marie Curie, EMBO, HFSP, Humboldt...

Our contribution to Mohit Misra, Ivan Đikić, and Co's stellar research on how a pathogenic bacterium, Legionella pneumophila, hijacks host cell signaling

It was a huge effort and took many years to combine all the data and methods, so enjoy reading👇
www.researchsquare.com/article/rs-6...