2: NIPBL regulates distinct sets of lineage-related genes across different cell types, likely by facilitating a unique spatial organization only at these select genes. This includes strong enhancer-promoter contacts and reduced insulation across the TSS, possibly reflecting multi-way contact hubs.
1.2: Loops rapidly lost with NIPBL depletion (transient) are associated with active promoter chromatin states, whereas the persistent loops are associated with H3K27me3-marked (Polycomb) chromatin, which may influence the stability of cohesin at this subset of loops.
1.1: A group of cohesin- & CTCF-dependent chromatin loops persist for >4 hours in the absence of NIPBL, but require
NIPBL for establishment during mitotic exit. The persistence of these loops is increased by STAG2 knockout & decreased by STAG1 knockout. We speculate that these are long-lived loops.
Very excited to share my postdoc research in the @jesserdixon.bsky.social lab at @salkinstitute.bsky.social, out online at @natgenet.nature.com today! www.nature.com/articles/s41... We investigated the function of the cohesin accessory protein NIPBL, making two particularly interesting findings:
2: NIPBL regulates distinct sets of lineage-related genes across different cell types, likely by facilitating a unique spatial organization only at these select genes. This includes strong enhancer-promoter contacts and reduced insulation across the TSS, possibly reflecting multi-way contact hubs.
1.2: While more transient loops are typically associated with active promoter chromatin states, the persistent loops are associated with H3K27me3 (Polycomb) repressive chromatin, which may act to influence the stability of cohesin and this subset of loops.
1.1: A group of cohesin- & CTCF-dependent chromatin loops persist for >4 hours in the absence of NIPBL, but require NIPBL for establishment during mitotic exit. The persistence of these loops is increased by STAG2 knockout & reduced by STAG1 knockout. We speculate that these are long-lived loops.
