Veesler lab

🚨 new paper alert 🚨

Here we demonstrate increased anti-RBS neutralizing responses elicited by stabilized influenza H5 immunogens:

bit.ly/4u8UxJl

Big thanks to Aakanksha @singhvilabglia.bsky.social for the nomination, to J. Bai and @nlehrbach.bsky.social for supporting it, to A. Calixto, S. Karimzadegan, P. Gurung, J. Cattin-Ortola, M. Crawford, for their supportive letters and to all my mentees for making this job colorful and meaningful ♥️

Functional and antigenic constraints on the Nipah virus fusion protein | PNAS Nipah virus is a highly pathogenic virus in the family Paramyxoviridae that utilizes two distinct surface glycoproteins to infect cells. The recept...

In new study led by Brendan Larsen, we map functional constraint across the Nipah virus F protein to define constrained epitopes for antibody targeting and identify mutations that stabilize the prefusion conformation for vaccine immunogens.

www.pnas.org/doi/10.1073/...

In new study we examine constraints on evolution of endemic "common-cold" coronavirus 229E

We find strong tradeoff between antibody neutralization & receptor binding, due to up-down position of spike RBD

www.biorxiv.org/content/10.6...

See lead author Sheri Harari's summary: x.com/SheriHarari/...

Our latest paper shows that IFITM3 deficiency allows SARS-CoV-2 to adapt more easily to a new host species. Mouse-adapted beta and omicron variants from this work maintained their unique tropisms and pathogenic characteristics allowing exciting comparative studies.
www.nature.com/articles/s41...

As Fran was nice enough to highlight this, I thought I'd put up a brief thread. 3 in 10 proteins that our cells make are either embedded in membranes (ion channels, adhesion molecules, etc.) or secreted (insulin, antibodies). They move through the secretory pathway.
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We studied how the glycan shield of alphaherpesvirus fusion protein gB mediates host co-receptor interactions. Great work by Sabrina, check it out!
www.biorxiv.org/content/10.6...

Our paper on the role of #SARSCoV2 NSP3 in stress granule control is now out in JVI.

Reposting our original BioRXIV thread on the summary.

journals.asm.org/doi/10.1128/...

Potent neutralization of Marburg virus by a vaccine-elicited antibody

www.nature.com/articles/s41...

Potent neutralization of Marburg virus by a vaccine-elicited antibody @nature.com @hhmi.org @veeslerlab.bsky.social @virbiotechnology.bsky.social
www.nature.com/articles/s41...

Delighted to share the peer-reviewed version of our article describing a prefusion-stabilized MARV GP vaccine immunogen and a best-in-class MARV neutralizing and protective antibody!

Led by @aminaddetia.bsky.social with @virbiotechnology.bsky.social

@hhmi.org

www.nature.com/articles/s41...

Since early 2024 we have been working with @brukercorporation.bsky.social on the first prototype timsOmni instrument, integrating an Omnitrap into a timsTOF. Here we report for the first time on the use of this instrument for protein-centric antibody sequencing. See www.biorxiv.org/content/10.1...

Mysteries of trafficking of the vRNP segmented genome and assembly of influenza A virus revealed by in situ cryo-ET!
🔗 rdcu.be/eMmct
We are very excited that our paper is finally out!
🎉 Big congratulations to Moritz Wachsmuth-Melm and to everyone involved.

Repeated COVID-19 vaccine boosters elicit variant-specific memory B cells in humans The first exposure to a pathogen or an antigen profoundly impacts immune responses upon subsequent encounter with related pathogens. This immune imprinting explains that infection or vaccination with ...

Our latest work on #COVID immunity and imprinting in humans.
Led by Ale Tortorici and Kaiti Sprouse with @aminaddetia.bsky.social and several others!

www.biorxiv.org/content/10.1...

Structural basis of T-loop–independent recognition and activation of CDKs by the CDK-activating kinase Cyclin-dependent kinases (CDKs) are prototypical regulators of the cell cycle. The CDK-activating kinase (CAK) acts as a master regulator of CDK activity by catalyzing the activating phosphorylation o...

Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control.
www.science.org/doi/10.1126/...

Congratulations Jason! This is awesome

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.

www.nature.com/articles/s41...

I am excited to share our latest preprint presenting the structure of human NHE6, a key endosomal Na⁺/H⁺ exchanger involved in Christianson syndrome.

We bring together #cryo-EM, NMR, SAXS, lipidomics, and functional assays for an integrative analysis of NHE6 architecture, function, and regulation.

We sequenced the anti-RSV-F antibody 131-2a by mass spec and determined its epitope by cryoEM. New insights in postfusion specific binding and antigenic site I. Now published in ACS Infectious Diseases @pubs.acs.org.
pubs.acs.org/doi/10.1021/...
@xdh.bsky.social @hangryhobbit.bsky.social

Our entrance into protein design! Inverse folding steered by external sources of information, and for multiple conformations. By the amazing @kaiyi94.bsky.social and @kjamali.bsky.social!
Download our code and try it yourself. 🥳

Loss of FCoV-23 spike domain 0 enhances fusogenicity and entry kinetics - Nature The molecular mechanisms of cell entry for the recently identified highly pathogenic feline coronavirus FCoV-23 are characterized in detail.

Important insights into FCoV-23 cell entry from the @veeslerlab.bsky.social

Freely available: www.nature.com/articles/s41...

Loss of FCoV-23 spike domain 0 enhances fusogenicity and entry kinetics pubmed.ncbi.nlm.nih.gov/40634609/ #cryoEM

Loss of FCoV-23 spike domain 0 enhances fusogenicity and entry kinetics - Nature The molecular mechanisms of cell entry for the recently identified highly pathogenic feline coronavirus FCoV-23 are characterized in detail.

Our paper describing how in-host evolution of a recently emerged, highly pathogenic coronavirus modulates entry into cells, membrane fusion and likely pathogenicity has now been published in its peer-reviewed form!

Led by Ale Tortorici!

@hhmi.org

www.nature.com/articles/s41...

Our effort to try to do something for [cystic fibrosis (CF)] started as a basic science enterprise. That was a hard slog back in the 1980s. Nobody would have supported except NIH. Now, 30 years after that discovery, young people with CF get to plan for retirement instead of an early funeral.

The 2025 Freeman Hrabowski Scholars | HHMI Freeman Hrabowski Scholars are outstanding early career faculty who have the potential to become leaders in their research fields.

Congratulations @stanford-chemh.bsky.social Institute Scholar @cobarnes27.bsky.social on being named a 2025 Freeman Hrabowski Scholar @hhmi.org 🎉 🍾

www.hhmi.org/programs/fre...

I have exhorted others to share positive news even in these uncertain times for US science.

I am really excited that @tamanash.bsky.social’s first paper on the remarkable evolutionary gymnastics of dual-host viruses (even in single codons) is now online.

Follow along here 🧪🧵

Potent neutralization of Marburg virus by a vaccine-elicited monoclonal antibody Marburg virus (MARV) is a filovirus that causes a severe and often lethal hemorrhagic fever. Despite the increasing frequency of MARV outbreaks, no vaccines or therapeutics are licensed for use in hum...

Outbreaks of Marburg virus (MARV) disease have been increasingly more frequent recently. Here we describe a prefusion-stabilized MARV GP and a best-in-class MARV neutralizing antibody!

Led by @aminaddetia.bsky.social with @virbiotechnology.bsky.social

@hhmi.org

www.biorxiv.org/content/10.1...

SARS-CoV-2 nsp1 mediates broad inhibition of translation in mammals Gen et al. demonstrate that SARS-CoV-2 nsp1 potently blocks translation in Rhinolophus lepidus bat cells and several other mammalian cell lines, concurring with the broad species tropism of SARS-CoV-2...

The peer-reviewed version of our article can now be found here and it is open access!

www.cell.com/cell-reports...

Fantastic team effort involving Kaitlin Sprouse, Mary-Jane Navarro, Jack Brown, Lexi Walls, Jimin Lee, Albert Seo, Cameron Stewart, Ben Merz, Samantha Zepeda, along with @kinglabipd.bsky.social Baric lab @tylernstarr.bsky.social lab Moderna SK Bioscience & many others!

Phylogeny-driven design of broadly protective sarbecovirus receptor-binding domain nanoparticle vaccines Vaccines against emerging SARS-CoV-2 variants and sarbecoviruses with pandemic potential must elicit a robust humoral immune response in a population imprinted with the SARS-CoV-2 spike (S) protein. H...

Delighted to share our most recent work on designing broadly protective sarbecovirus vaccines and defining the path forward for other coronaviruses.

Led by @aminaddetia.bsky.social

@hhmi.org

www.biorxiv.org/content/10.1...