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Manon Demulder

@manondemulder

Incoming Young Group Leader GMI - Vienna Biocentre | Current postdoc in Engel Lab| All things carbon fixation and algae diversity zoomed in β„οΈπŸ”¬πŸͺΈ

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20.10.2023
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Latest posts by Manon Demulder @manondemulder

Do you like it when weird organisms do silly things?? Then you're going to love this!

05.03.2026 21:03 πŸ‘ 21 πŸ” 7 πŸ’¬ 3 πŸ“Œ 0
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An unconventional Rubisco small subunit underpins the CO2-concentrating organelle in land plants In many algae, photosynthesis is boosted by biophysical CO2-concentrating mechanisms, which pack the CO2-fixing enzyme Rubisco into liquid-like organelles called pyrenoids. Engineering C3 crops with a...

Our work on #hornwort #pyrenoids is finally out in @science.org! πŸŽ‰ We uncovered how hornworts pack their Rubisco into pyrenoids and successfully recreated them in Arabidopsis. A key step toward engineering more efficient photosynthesis in crops @btiscience.bsky.social www.science.org/doi/10.1126/...

05.03.2026 20:03 πŸ‘ 122 πŸ” 62 πŸ’¬ 6 πŸ“Œ 7

Apply now! @vbcscitraining.bsky.social offers more than 25 fully-funded PhD positions! GMI group leaders are also recruiting new students.

04.03.2026 15:33 πŸ‘ 4 πŸ” 9 πŸ’¬ 0 πŸ“Œ 0
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Interested in the most recent developments in plant science? Join the Mendel Early Career Symposium on May 21-22 at the @viennabiocenter.bsky.social to exchange with experts about their science and career trajectories. Register by March 13th for a talk. More here: www.oeaw.ac.at/gmi/news-eve...

24.02.2026 14:01 πŸ‘ 20 πŸ” 13 πŸ’¬ 0 πŸ“Œ 1
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@embl.org at its best: collaborative, open, transformative, and just the right amount of crazy. Dreaming of mechanistic cell biology *in the natural environment*? Dream no more, rent the AML.

www.biorxiv.org/content/10.6...

www.youtube.com/watch?v=jepb...

#WeFreezeOnTheBeach #ProtistsOnSky 🦠🌍πŸ§ͺ

24.02.2026 12:34 πŸ‘ 86 πŸ” 29 πŸ’¬ 3 πŸ“Œ 2
Fig. 2 Life cycle of Cyanidiophyceae.

Fig. 2 Life cycle of Cyanidiophyceae.

✨ Paper spotlight ✨

(🧡 1/2) Fascinating single cell red algae: models for evolution and adaptation
nph.onlinelibrary.wiley.com/doi/10.1111/...

#PlantScience

20.02.2026 13:02 πŸ‘ 12 πŸ” 7 πŸ’¬ 1 πŸ“Œ 0
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Thermophilic bacteria employ a contractile injection system in hot spring microbial mats Abstract. Bacterial contractile injection systems (CISs) are multiprotein complexes that facilitate the bacterial response to environmental factors or inte

Contractile Injection Systems - Hot Springs - cryoET: Read the final version of our paper here:
academic.oup.com/ismej/advanc...
#microsky #teamtomo

11.02.2026 14:43 πŸ‘ 27 πŸ” 9 πŸ’¬ 0 πŸ“Œ 1
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Long in the making, but happy to present the Chlamydomonas chlororibosome!
Cryo-ETπŸ”¬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins.
bioRxiv πŸ“–: shorturl.at/q44tG
This suggests greater chlororibosome diversity than expected!
1/n 🧡

10.02.2026 08:35 πŸ‘ 154 πŸ” 54 πŸ’¬ 4 πŸ“Œ 6
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Spatial Proteomics Workshop for Protists

(see more in thread below)

Registration
Early Bird Monday 22nd June 2026.
Regular Monday 10th August 2026.
There will be a conference dinner on day 1. To register visit protistology.org.uk/autumn-meeti...

06.02.2026 18:11 πŸ‘ 25 πŸ” 17 πŸ’¬ 1 πŸ“Œ 0

You like #LLPS? #TeamTomo? #Rubisco? Check out our latest preprint!

What a great collaboration this has been!

30.01.2026 15:38 πŸ‘ 30 πŸ” 11 πŸ’¬ 0 πŸ“Œ 0
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How does molecular valency shape condensate assembly and function? We used the CO2-fixing organelle in algaeβ€”the pyrenoidβ€”to find out… 🧡

Preprint here!:
www.biorxiv.org/content/10.6...

@cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social
#Rubisco #PhaseSeparation #Condensates #Pyrenoid

30.01.2026 15:20 πŸ‘ 78 πŸ” 37 πŸ’¬ 1 πŸ“Œ 5
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How do diatom cells maintain a constant supply of carbon for photosynthesis? Our microelectrode measurements of the phycosphere around individual diatom cells show that diatoms can rapidly switch between distinct carbon acquisition pathways. @thembauk.bsky.social www.science.org/doi/10.1126/...

14.01.2026 09:49 πŸ‘ 24 πŸ” 10 πŸ’¬ 1 πŸ“Œ 0
Sticker number modulates pyrenoid condensate assembly to support algal fitness The valency of intrinsically disordered proteins underpins liquid-liquid phase separation (LLPS), yet how this parameter shapes condensate function and cellular fitness remains poorly understood. Here we exploit the algal pyrenoidβ€”a minimal, two component LLPS systemβ€”to directly link condensate properties to physiological performance. Pyrenoid assembly is driven by a disordered, multivalent Linker protein that binds Rubisco at symmetry-related surface sites, with the number of binding motifs (β€³stickersβ€³) varying across species. Using Chlamydomonas reinhardtii , we systematically tuned sticker number from two to nine and examined effects on Rubisco condensation, pyrenoid architecture and CO2 fixation. Three stickers were sufficient for condensation in vitro, but at least four were required for pyrenoid assembly in vivo. Cryo-electron tomography and single-molecule tracking revealed that increasing sticker number enhances Rubisco packing and mobility, while time-resolved imaging and competition assays demonstrated that sticker number governs the kinetics of pyrenoid formation and determines cellular fitness under fluctuating carbon conditions. Our findings establish sticker number as an evolutionary tuning parameter that balances condensate formation, dynamics, and function, providing a quantitative framework for linking the molecular grammar of phase separation to biological fitness. ### Competing Interest Statement The authors have declared no competing interest. EPSRC, EP/W024063/1

Sticker number modulates pyrenoid condensate assembly to support algal fitness

Looking forward to reading this! @james-r-barrett.bsky.social @phaips.vd.st ps.vd.st

www.biorxiv.org/content/10.6...

30.01.2026 03:58 πŸ‘ 6 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
Researcher Florian Wollweber standing next to a cryo-focused ion beam microscope in a laboratory, with technical infrastructure visible in the background.

Researcher Florian Wollweber standing next to a cryo-focused ion beam microscope in a laboratory, with technical infrastructure visible in the background.

The origin of eukaryotic cells continues to be one of the greatest mysteries in biology.

EMBL Grenoble is excited to welcome Florian Wollweber, who will use cutting-edge imaging techniques to study Asgard archaea β€” microbes closely related to our earliest ancestors. πŸ¦ πŸ”¬

www.embl.org/news/people-...

28.01.2026 10:56 πŸ‘ 86 πŸ” 20 πŸ’¬ 4 πŸ“Œ 0
Poster of the EM course in Naples with marine samples. The Electron Microscopy Hands-On Course: sample preparation and imaging of marine environmental samples arises from a very strong and active collaboration between the Stazione Zoologica Anton Dohrn and the EMBL in Heidelberg.
The aim of the course is to provide a theoretical and practical training on electron microscopy starting from the basics to the preparation of samples with high pressure freezing and image acquisition using TEM and SEM.

Poster of the EM course in Naples with marine samples. The Electron Microscopy Hands-On Course: sample preparation and imaging of marine environmental samples arises from a very strong and active collaboration between the Stazione Zoologica Anton Dohrn and the EMBL in Heidelberg. The aim of the course is to provide a theoretical and practical training on electron microscopy starting from the basics to the preparation of samples with high pressure freezing and image acquisition using TEM and SEM.

If you are interested in electron microscopy, registration is still open for the

"Electron Microscopy Hands-On Course: sample preparation and imaging of marine environmental samples"

at Stazione Zoologica in Naples.

WIth great teachers from Naples […]

[Original post on biologists.social]

13.01.2026 07:07 πŸ‘ 31 πŸ” 32 πŸ’¬ 0 πŸ“Œ 1
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Pyrenoid Structure, Function, Evolution, and Characterization Across Diverse Lineages Pyrenoids are eukaryotic CO2-fixing organelles that are evolutionarily diverse, globally abundant, and critical to global carbon cycling. Despite being described over 200 years ago, the vast majority ...

Pyrenoid Structure, Function, Evolution, and Characterization Across Diverse Lineages.

www.annualreviews.org/content/jour...

17.01.2026 02:22 πŸ‘ 9 πŸ” 4 πŸ’¬ 0 πŸ“Œ 0

This one was quite the journey! The paper describing the #ChlamyDataset is finally out and on the cover of Mol Cell!

This beautiful rendering made by co-author @jessheebner.bsky.social and Holly Peterson shows an instance of mitochondrial fission found in the dataset 😍

[Maybe long thread ahead]

09.01.2026 19:14 πŸ‘ 92 πŸ” 35 πŸ’¬ 3 πŸ“Œ 10

CryoET of microbes inside an animal organ? Yes it’s possible! Check out our new preprint showing how we did it! What an amazing collaboration with amazing scientists who made this possible!

01.12.2025 04:35 πŸ‘ 123 πŸ” 35 πŸ’¬ 2 πŸ“Œ 4
Station 2  | SOI Divestream 863
Station 2 | SOI Divestream 863 YouTube video by Schmidt Ocean

Livestream dive two. We see a strawberry squid and a sea of brittle stars: www.youtube.com/watch?v=HZLe...

15.11.2025 10:01 πŸ‘ 21 πŸ” 5 πŸ’¬ 1 πŸ“Œ 0
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We are looking for a PhD student to work on an exciting plastid endosymbiosis in microbial eukaryotes. This position involves sampling, exciting microscopy such as CARDFISH, ExM and FIBSEM, single-cell transcriptomics and more. #protistsonsky 1/2

12.11.2025 09:50 πŸ‘ 79 πŸ” 66 πŸ’¬ 2 πŸ“Œ 3
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Exciting day for the lab: our 1rst paper is officially out in @currentbiology.bsky.social πŸ₯³ Wonderful collaboration wt @gautamdey.bsky.social showing how Cryo-ExM achieves consistent immunostaining in diverse diatoms, from the lab and the natural environment 1/n
#ProtistsOnSky
tinyurl.com/2zxaund7

31.10.2025 15:27 πŸ‘ 166 πŸ” 55 πŸ’¬ 1 πŸ“Œ 2
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🚨Our collaboration with @centriolelab.bsky.social & @gautamdey.bsky.social is out today in @cp-cell.bsky.social
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky

www.cell.com/cell/fulltex...

31.10.2025 14:41 πŸ‘ 215 πŸ” 98 πŸ’¬ 15 πŸ“Œ 11

Please join us next week for the second iteration of the DinoSphere Seminars! This time our two speakers will talk about host-associated dinoflagellates and any challenges they might have encountered when working with them. All current and future friends of dinoflagellates welcome!
#ProtistsOnSky

29.10.2025 09:19 πŸ‘ 12 πŸ” 7 πŸ’¬ 0 πŸ“Œ 0
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We’re back with the next DinoSphere Online Seminar!

Join us on Nov 4th, we're hosting:

Edmée Royen (ULiège) - Symbiodinium

Nicolas dos Santos Pacheco (Cambridge) - Perkinsids

πŸ“… Tue, Nov 4 - 4 PM CET/3 PM GMT
β€¨πŸ’» Zoom link: sites.google.com/view/dinosphere


Please spread the word!
#protistonsky

28.10.2025 15:29 πŸ‘ 19 πŸ” 16 πŸ’¬ 0 πŸ“Œ 2
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After exploring Europe’s coastlines, EMBL’s TREC expedition is moving inland! πŸ’§

The new Traversing Ecosystems phase focuses on freshwater habitats – studying how human activity and environmental change affect life at the molecular level.

www.embl.org/news/science...

23.10.2025 09:02 πŸ‘ 26 πŸ” 8 πŸ’¬ 0 πŸ“Œ 0
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Locating the missing chlorophylls f in far-red photosystem I The discovery of chlorophyll f-containing photosystems, with their long-wavelength photochemistry, represented a distinct, low-energy paradigm for oxygenic photosynthesis. Structural studies on chloro...

Have you ever looked at the night sky and wondered if there’s a chlorophyll f in the reaction centre of Photosystem I from far-red light adapted cyanobacteria? 🦠

Well, we did (don’t judge) and the work that followed is now out as a First Release in @science.org

www.science.org/doi/10.1126/...

11.10.2025 10:37 πŸ‘ 64 πŸ” 19 πŸ’¬ 2 πŸ“Œ 2
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Pretty excited to share our new preprint!
Non-photosynthetic Plastid Replacement by a Primary Plastid in the Making
www.biorxiv.org/content/10.1...

10.10.2025 03:34 πŸ‘ 91 πŸ” 45 πŸ’¬ 0 πŸ“Œ 3
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Ice Diatoms Glide at Record-Low Temperatures - Eos New observations reveal how microscopic organisms move through polar ice and illustrate how they may have evolved to thrive in extreme environments.

Life knows how to keep going - even if it’s freezing -15 degrees C πŸ₯Ά outside. AGU just covered our discovery of ultra resistant ice diatoms gliding into a world record! πŸ§ͺπŸ”¬Enjoy the story here:

eos.org/articles/ice...

07.10.2025 15:39 πŸ‘ 40 πŸ” 13 πŸ’¬ 0 πŸ“Œ 4

EXCITING NEWS!! We're searching for a new professor colleague at the BiozentrumπŸ‡¨πŸ‡­. Ideal candidates are exploring novel biological questions with structure, biophysics, or high-resolution imaging. πŸ§ͺ 🧢🧬 πŸ”¬

The call closes Nov 3. Please get in touch if you would like more information. Come join us! πŸ‘©β€πŸ”¬πŸ€“

30.09.2025 09:52 πŸ‘ 58 πŸ” 60 πŸ’¬ 2 πŸ“Œ 4

Check out the work our lab’s photosynthesis brain
@wojwie.bsky.social
and @lorenzlamm.bsky.social who created the AI pipeline that made this possible

25.09.2025 19:40 πŸ‘ 6 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0