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Norman Rzepka

@normanrz.com

Co-Founder of scalable minds. Building WEBKNOSSOS, a tool visualizing, annotation, managing and sharing of large 3D images. Contributing to OME-Zarr. he/him @normanrz@mastodon.social

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Latest posts by Norman Rzepka @normanrz.com

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Run pre-trained segmentation models for SEM data in WEBKNOSSOS.

Test on small bounding boxes, inspect merge and split errors, and run on the full dataset once validated.

View the tutorial: youtu.be/lZS2tOJYErw

05.03.2026 13:30 πŸ‘ 1 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
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We annotated ground truth data and trained an instance segmentation model for mitochondria detection on a 140 GB mouse cortex dataset (Motta et al., 2019) in WEBKNOSSOS.

See the workflow and results: medium.com/webknossos/c...

26.02.2026 15:30 πŸ‘ 2 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
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syGlass Winter 2026 Release: v2.6.0 β€” syGlass Although it comes on the heels of our last feature release less than three months ago, syGlass v2.6.0 has no shortage of new content to help create new workflows and streamline old ones. Read on to se...

The first syGlass feature release of the year is here! Meet syGlass v2.6.0:

πŸ–₯️ Spatial Reality Display support
πŸ›œ Stream/read OME-Zarr directly
πŸ–ŒοΈ Partition surfaces via 3D brush
⌨️ Enter text via in-VR keyboards

www.syglass.io/blog/syglass...

17.02.2026 23:55 πŸ‘ 1 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
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Last year brought many great new features to WEBKNOSSOS ✨
Over the next days, we’ll share a series of feature highlights. Stay tuned!

15.01.2026 13:01 πŸ‘ 0 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
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NGFF weekly dev update thread 🏁 Sprint to 0.6 🏁 Dear NGFF-community, it's been some time since the last release of a mjor version of the NGFF-specification. Since then, many of you engaged with the community which resulted in a lo...

🚨Gearing up for #NGFF 0.6! 🚨

Help us give this a final push over the finish line and join us in ironing out the last flaws on the way!

More information here πŸ‘‡
forum.image.sc/t/ngff-weekl...

09.01.2026 13:48 πŸ‘ 4 πŸ” 2 πŸ’¬ 0 πŸ“Œ 0
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Method of the Year 2025: electron microscopy-based connectomics - Nature Methods A large network of interconnected neurons serves as the basis of brain function and of behavior. Methodological advances have enabled the reconstruction of large-scale and even whole-brain connectomes...

Our Method of the Year 2025 is...drumroll please...EM-based connectomics!!

Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! πŸŽ‰πŸ§ πŸ”¬

www.nature.com/articles/s41...

08.12.2025 16:59 πŸ‘ 52 πŸ” 21 πŸ’¬ 1 πŸ“Œ 2
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Connectomics is Method of the Year 2025!!
@natmethods.nature.com
just released the news nature.com/articles/s41... In my perspective piece, I afford some predictions into the future and compare how we are doing vs. genomics www.nature.com/articles/s41...

08.12.2025 16:44 πŸ‘ 27 πŸ” 16 πŸ’¬ 1 πŸ“Œ 0
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Maybe @normanrz.com can provide more @webknossos.org socks to help with the shoe-filling?

04.12.2025 18:17 πŸ‘ 1 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0

Thanks so much for all your work organizing this great symposium series over the years!
I am already looking forward to next year’s edition.

03.12.2025 07:30 πŸ‘ 1 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0
Nondestructive X-ray tomography of brain tissue ultrastructure - Nature Methods Optimizations of X-ray nanotomography including the choice of resin allows high-resolution imaging of mouse brain tissue, approaching the resolution of volume electron microscopy. Since it does not re...

#paper!
X-rays can resolve #ultrastructure in tissues non-destructively.

www.nature.com/articles/s41...

Shoutout to key collaborators at @crick.ac.uk and @psich.bsky.social Ana Diaz, @adrianawanner.bsky.social and @andreas-t-schaefer.bsky.social, and to the whole team that made this possible.

27.11.2025 11:51 πŸ‘ 10 πŸ” 7 πŸ’¬ 1 πŸ“Œ 1
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Happy to share the first paper from my journey at @psich.bsky.social towards X-ray connectomics, now out in @natmethods.nature.com: www.nature.com/articles/s41...

27.11.2025 10:21 πŸ‘ 39 πŸ” 15 πŸ’¬ 1 πŸ“Œ 0
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Our new paper showcasing molecular connectomics with pan-expansion microscopy is out in @natbiotech.nature.com! www.nature.com/articles/s41...
This wonderful collaboration with @bewersdorflab.bsky.social was led by Ons M'Saad (now founder/CEO of Panluminate) and @allisonphysics.bsky.social. (1/5)

27.11.2025 04:39 πŸ‘ 61 πŸ” 24 πŸ’¬ 1 πŸ“Œ 0
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An Introduction to OME-Zarr for Big Bioimaging Data

Today I'm announcing a new digital textbook πŸ“–πŸ–₯️, "An Introduction to OME-Zarr for Big Bioimaging Data".

ome-zarr-book.readthedocs.io

04.11.2025 15:33 πŸ‘ 30 πŸ” 16 πŸ’¬ 2 πŸ“Œ 0
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🌟Enhancements🌟

The proofreading tool got smarter: you can now define multiple segments around a desired split, and it will automatically pick the optimal split point.

09.10.2025 12:48 πŸ‘ 5 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
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🌟Enhancements🌟

Shared annotations got a big upgrade: they now update liveβ€”see your collaborators’ changes as they happen. Simultaneous editing is on the horizon πŸ‘€

18.09.2025 12:48 πŸ‘ 3 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
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Hard X-ray imaging of biological soft tissues symposium 2025

#Nanoimaging symposium @crick.ac.uk!

At #CrickXrays25 we'll tackle:
πŸ’«synchrotron upgrades
πŸ“ˆcurrent limits in throughput, volume, resolution
πŸ§ͺsample preparation for X-ray PC imaging
🧠β™₯️applications in neuroscience and tissue life science

πŸ—“οΈ13th October 2025
πŸ‡¬πŸ‡§πŸŒ London & online
tinyurl.com/crickxrays25

23.09.2025 10:43 πŸ‘ 10 πŸ” 7 πŸ’¬ 1 πŸ“Œ 1

Want to hack with ~40 motivated developers in the OME-Zarr space this fall in Zurich, November 12 - 14th? The application window for the 2025 OME-NGFF workflows hackathon is closing on September 30th! Apply here to be part of this exciting week about OME-Zarr: forms.gle/849cf2fBk1pJ...

18.09.2025 09:42 πŸ‘ 11 πŸ” 11 πŸ’¬ 0 πŸ“Œ 0
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πŸŽ‰ Our paper β€œMIFA: Metadata, Incentives, Formats and Accessibility guidelines to improve the reuse of AI datasets for bioimage analysis” is out in @natmethods.nature.com!
Community-driven standards to make bioimage data AI-ready & reusable.
πŸ‘‰ www.nature.com/articles/s41... #AI #Bioimaging #FAIRdata

15.09.2025 09:57 πŸ‘ 16 πŸ” 8 πŸ’¬ 1 πŸ“Œ 1
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CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy - Nature Methods CLEM-Reg automates the three-dimensional alignment of volume correlative light and electron microscopy datasets by leveraging probabilistic point cloud registration techniques for fast and accurate re...

Super happy to see CLEM-Reg out in @natmethods.nature.com! A short thread on the algorithm behind it and how to try it out for yourself🧡(1/9)

www.nature.com/articles/s41...

10.09.2025 09:37 πŸ‘ 43 πŸ” 20 πŸ’¬ 2 πŸ“Œ 2

#preprint:

We optimised the preparation of tissues for #X-ray phase contrast imaging.

Drying tissue samples (instead of embedding them) brings more signal in #X-ray phase contrast images.

The process largely preserves #ultrastructure.

@safekhan.bsky.social @andreas-t-schaefer.bsky.social

10.09.2025 15:51 πŸ‘ 10 πŸ” 4 πŸ’¬ 1 πŸ“Œ 0
Visual of segmented human induced pluripotent stem cells with the key workshop dates: Symposium on November 10-11, Hackathon on November 12-14th in Zurich Switzerland

Visual of segmented human induced pluripotent stem cells with the key workshop dates: Symposium on November 10-11, Hackathon on November 12-14th in Zurich Switzerland

We're happy to announce the 2025 International OME-NGFF workshop πŸŽ‰

πŸ“… Dates:
- Symposium: Nov 10–11, 2025
- Hackathon: Nov 12–14, 2025

πŸ“ Location: Zurich, Switzerland

Full details & registration: www.biovisioncenter.uzh.ch/en/events/Up...Β 

#bioimaging #openscience #OMENGFF #OMEZarr

(1/x 🧡)

25.08.2025 09:07 πŸ‘ 40 πŸ” 32 πŸ’¬ 1 πŸ“Œ 4
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🌟Enhancements🌟

ICYMI: Webknossos viewports are now rotatable.

Use keyboard shortcuts to rotate the orthogonal viewport. Try Shift + Left, Shift + Right, Shift + Up and Shift + Down.

Learn more about keyboard shortcuts: docs.webknossos.org/webknossos/u...

14.08.2025 15:47 πŸ‘ 1 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
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🌟Big new feature🌟

Rotate the viewports. You can now freely rotate the orthogonal viewports. Trace your objects in the best angle.

31.07.2025 15:06 πŸ‘ 4 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
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🌟Enhancement🌟

Make the 3D meshes of your segments translucent. Great for revealing inner segments, such as as cell organelles. Also, translucent meshes look really cool 😎

17.07.2025 14:27 πŸ‘ 7 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
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Hey bioimaging folks, join us for the next OME-NGFF Community Call on June 24 at 9:00 CEST and 17:00 CEST!

Details and agenda on the one and only image.sc here: forum.image.sc/t/join-us-fo...

03.06.2025 10:17 πŸ‘ 10 πŸ” 10 πŸ’¬ 0 πŸ“Œ 0

OME-Zarr 4 life

13.05.2025 14:21 πŸ‘ 9 πŸ” 3 πŸ’¬ 1 πŸ“Œ 0
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WEBKNOSSOS: Visualize, share and annotate your large 3D images online Visualize, share, and annotate your large 3D images online. Upload your data and share links with colleagues. Collaboratively create skeleton and volume annotations, all from your browser. Optimized f...

You can try it on webknossos.org and if you want to set it up on your own, check out docs.webknossos.org/webknossos/o...

12.05.2025 12:20 πŸ‘ 2 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

You can now apply until the 4th of June !!
#EMBOBioImage www.embl.org/about/info/c...

05.05.2025 17:54 πŸ‘ 9 πŸ” 10 πŸ’¬ 0 πŸ“Œ 1

See you there!

01.05.2025 17:12 πŸ‘ 1 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0
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#preprint:

We mapped the structure and function of olfactory bulb circuits with in vivo 2-photon microscopy and synchrotron #Xray holographic nanotomography.

doi.org/10.1101/2025...

@yuxinzhang.bsky.social @andreas-t-schaefer.bsky.social @apacureanu.bsky.social
@crick.ac.uk @esrf.fr

01.05.2025 13:38 πŸ‘ 62 πŸ” 22 πŸ’¬ 1 πŸ“Œ 4