This came up on stream earlier, so I should ask you guys:
Do you think it's okay to stream with a sillhouette model before the release? Or should it be kept to social media spoilers only?
#vtuber #vtubermodel #communityquestions
My characters are trapped in a dungeon full of mana stones and only have basic weapons.
What should they do?😰
Let's help them🥺
#TTRPG #DnD #CommunityQuestions
Question to the lovelies 💚
Do you prefer?
1. Multiple channels/accounts for specific content?
2. A single, well managed, channel/account with everything a creator does?
#vtuber #ENVtuber #communityquestions #streamer #twitch #youtube #soundcloud #promosky #indieproducer #music
#nanopore #communityQuestions
"""
* Does running multiple pore checks affect the pores in the flow cell in any way?
* Why are total pores higher than available pores?
* What length of reads block or damage pores?
"""
- Natalie hajduga
#nanopore #communityQuestions
Can Adaptive Sampling be used to multiplex samples for human mitochondrial DNA sequencing on MinION? Samples contain both nuclear and myDNA. If yes, what maximum number of samples can be multiplexed? - Prince Adoba
#nanopore #communityQuestions
There are frequent questions on the nanopore community forums about people wanting to do very specific things with the latest unpublished technology, and asking for advice prior to trying anything out.
"The native adapter (NA) seems to be a limiting factor, as only 40µl is provided per kit, and 5µl are needed per sequencing run. When running very few barcodes, the NA runs out quickly.
Is it possible to order the native adapter separately?" - Balal Sadeghi
#nanopore #communityQuestions
#nanopore #communityQuestions
"We are trying to optimize using the native barcoding kit for plant genomic DNA multiplexed runs. It's often the case that one of the samples is overrepresented in the demultiplexed reads. Did anyone else encounter this bias? What could be the cause?" - Ari Meerson
#nanopore #communityQuestions
"How can one calculate the estimated sequencing time for amplicons barcoded using transposomes and those natively barcoded?"
- Younis Skaik
#nanopore #communityQuestions
"test 1 test 1 test 1 test 1 test 1 test 1 test 1 test 1 test 1 test 1" - Nick Brain
#nanopore #communityQuestions
"Is it recommended to run a Lambda control run on a newly purchased P2 Solo? For how long?"
"I have these NEB reagents (Blunt/TA Ligase Master Mix, End repair/dA-tailing Module and Quick Ligation Module); can I follow our amplicon sequencing protocol?"
- Paola Niola
#nanopore #communityQuestions
"Has anyone had luck using the transposon based rapid barcoding kit to tagment plasmids, then anneal a native barcode from the ligation kit to this, pre-adaptor, and finish off then sequence with the ligation-based kit?" - Integral Molecular
#nanopore #communityQuestions
"In the rapid plasmid sequencing protocol the load amount suggested is '50 ng high molecular weight plasmid DNA per sample'. What is 'high molecular weight referring to here? Is there a plasmid size cut-off under which this protocol wont function?" - Integral Molecular
#nanopore #communityQuestions
"I'm collecting longitudinal water samples for a year for taxonomic profiling, diversity analysis, and group comparative analysis between seasons. What's the difference between barcoding 24 or 96 samples in each run via native barcoding?" - Hanieh Shakeri Moghaddam
#nanopore #communityQuestions
"Is it possible to sequence more than one sample for DNA methylation analysis using adaptive sampling with the MinION sequencer?
We plan to analyze 500 methylation targets. How many samples can we multiplex per MinION flow cell?" - Aleksandra Pisarek-Pacek
#nanopore #communityQuestions
"Is there any way to design custom primers and so that the PCR product can be directly processed by the Rapid Adapter and subjected to V14 flow cells?" - Wei Wei
#nanopore #communityQuestions
"Does anyone know the shelf life of a mix of Rapid Adapter and Adapter Buffer from the Rapid Sequencing Kit V14 when stored at -20°C? I'm considering preparing a stock solution of the two that I can use over the course of a month." - Demi van Os
#nanopore #communityQuestions
"We are planning to start metagenomic sequencing of ticks and I am wondering if anyone in this community has any experience with tick metagenomics and can recommend a library preparation kit?" - Liisa Truu
