Lighting the Path to Neofunctionalization: Tuning Yeast Evolution with OptoRep The pursuit of rapid, targeted protein evolution has been significantly advanced by systems like OrthoRep, which allows for high-rate in vivo hypermutation of specific genes in Saccharomyces cerevisia...

Princeton's OptoRep, a system combining OrthoRep's hypermutation with optogenetic precision. Blue light controls essential metabolism, fine-tuning selection pressure, enabling de novo neofunctionalization. go.njmicrobe.org/9M8ssQ #Microbiology #MetabolicEngineering #Optogenetics #ContinuousEvolution