🧬 Just pushed new updates to #OpenCloning Assembler based on user feedback!
✍️ Now supports #GoldenBraid and Plant #MoClo
🚀 You can edit assembly product names, view a full syntax overview, and enjoy cleaner visuals.
👉 Try it out (link in comments) — feedback welcome!
#OpenCloning is shaping up to be a really useful tool, and the new Assembler is powerful for defining and using Golden Gate syntaxes. Give it a try!
Someone requested #GoldenBraid Assembly, now it's live on #OpenCloning. Still missing the omega Level 1 assembly (coming very soon).
For GoldenBraid reference: goldenbraidpro.com and pmc.ncbi.nlm.nih.gov/articles/PMC...
Do you like #cloning or #MolBiol?
Do you like #OpenSource software?
Do you like keeping structured research records in a way that can also save you time and help avoid mistakes?
If you answered 'Yes' to any of the above, then check out this new update to #OpenCloning from @manu-lera.bsky.social 👇
🚀 Excited to launch the #OpenCloning Assembler!
🧬 Built to demystify Golden Gate for beginners and save time for experts -> MoClo assembly blazing fast ⚡
✍️ Use existing syntaxes or design your own!
#SynBio #GoldenGate #MoClo #OpenSource #Biotech
👉 Try it: app.opencloning.org
Feedback welcome!
Working on a tool to define Golden Gate syntaxes for #OpenCloning. You define your syntax, and then you can drag-and-drop your plasmids in OpenCloning, they will be sorted into the right categories, and you can do combinatorial assembly. Reach out if you'd like to contribute:
syntax.opencloning.org
🚀 A long-requested feature just landed in #OpenCloning
🔬🧬 You can now manually annotate sequences and create primers right from the editor
✨ Hope it makes your workflow a bit smoother!
📨 If you want to stay updated with upcoming features, subscribe to the mailing list (link in the comments)
If you are looking for an Open and free alternative to SnapGene, you may want to give #OpenCloning a try!
bsky.app/profile/manu...
If you are looking for an Open and free alternative to SnapGene, you may want to give #OpenCloning a try!
bsky.app/profile/manu...
cc: crisprflydesign.bsky.social
If you are looking for an Open and free alternative to SnapGene, with a more modern look than ApE, you may want to give #OpenCloning a try!
bsky.app/profile/manu...
If you are looking for an Open and free alternative to SnapGene, you may want to give #OpenCloning a try!
bsky.app/profile/manu...
If you are looking for an Open and Free alternative to SnapGene, you may want to give #OpenCloning a try!
bsky.app/profile/manu...
A cloning strategy as depicted in the OpenCloning website
#OpenCloning is a an Open Source alternative to SnapGene/Benchling that supports automation and integration with other software
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
✨ Last week at #ESSL2025 I presented a poster on #OpenCloning 🧬
🔥 Crowd favorite feature: pull gene sequences directly from NCBI — no copy-paste needed!
🎥 Here’s a quick demo
🔗 Visit opencloning.org
#OpenCloning opencloning.org now features WeKwikGene integration—streamlining downloading plasmid sequences from wekwikgene.wllsb.edu.cn and downstream cloning and collaborations!
🚀 OpenCloning now has a documentation page!
Whether you're getting started or diving deep, find everything you need to make the most of OpenCloning, including a powerful search function to quickly find exactly what you’re looking for.
#OpenCloning #OpenSource #SynBio
🔗 docs.opencloning.org
🔬 I added a template for the #GoldenPiCS #MoClo kit to #OpenCloning!
⚡ Speed up your design when cloning in #Kphaffii!
🧪 Simulate plasmid assembly
🧬 …and integration into the genome!
👉 Check it out here: opencloning.org 🌍
🎥 youtu.be/BadWzLjojb4
🧪 Maybe it's time to give #OpenCloning a try!
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
👨🔬 Built by a fellow researcher — for researchers!
👉 Check it out: bsky.app/profile/manu...
I'm really excited that #OpenCloning has been integrated into @elabftw.bsky.social - we're just getting it setup on our development server, and I'm looking forward to seeing what our users will be able to do with this! Love the structure of your approach - keep up the good work!
🧬 If you are in London and you want to learn about a free & Open Source SnapGene/Benchling alternative that also supports automation 🤖, come to our next London Synbio Network meeting this Thursday 24th in Imperial!🎓
🎤 I’ll be giving a talk about #OpenCloning!
events.humanitix.com/copy-of-lond...
Come join us for the next London Synbio Network meeting on Thursday the 24th of April in Imperial.
I am giving a talk about #OpenCloning, so join if you want to hear more about Open Source and automation of DNA engineering.
Just got your sequencing results from Full Circle Labs? 🧬✅ Use #OpenCloning to easily align your Nanopore (or Sanger) data with your constructs and verify your sequences in seconds!
🔬 Free & Open Source
🛠️ Supports most cloning methods
🔗 opencloning.org
#SyntheticBiology #MolecularBiology
You can now ✅ verify your #OpenCloning sequences using sequencing data 🧬 (Sanger or Nanopore). Just load your files 📂 and see where they align! 🎯
#OpenCloning is free & Open Source, and supports most cloning methods! 🔬🛠️
👉 Try it here: opencloning.org
🎞️ Demo video: youtu.be/n0hedzvpW88?...
#OpenCloning now displays primer details! 🧬🔍 Minimal info is shown in the table, and the full #primer3 report when clicking the ℹ️ icon.
🧪 The values in parenthesis are for the full primer, the preceding ones for the annealing part.
🔗 Try it yourself: opencloning.org
🎥 Demo: youtu.be/-wjOY4i6mjg
With #OpenCloning, you can very quickly access your locus of interest from the #NCBI to start cloning. No more downloading entire genomes and cutting your fragment of interest!
🧬 Using Gateway for cloning? Plan it with #OpenCloning! 🖥️✨
🆓 Free & Open Source, it supports most cloning methods! 🔬🛠️
🎥 Demo: youtu.be/-wjOY4i6mjg
🧪 Try it yourself: opencloning.org
🤔 Why GateWayMine?
I'm building #OpenCloning, an 🧬 Open Source web app to plan & document cloning & genome engineering. 🖥️✨
To support Gateway Cloning, I needed consensus sequences for att sites—but they were nowhere to be found! 😱🔍
⛏️ So, I had to mine them! ⛏️
🧬 Now you can access @sevaplasmids.bsky.social directly from #OpenCloning ✨.
🔗 try it: opencloning.org
🎞️ demo: youtu.be/n0hedzvpW88?...
I have turned the primer designer of #OpenCloning into a stepper after feedback from lab members and @francescodb.bsky.social. It now conveys more clearly the workflow. Below an example of #primerDesign for #GatewayCloning
🔗 try it: opencloning.org
🎞️ demo: www.youtube.com/watch?v=n0he...
