Regulatory rewiring drives intraspecies competition in Bacillus subtilis Author summary Microbial competition and cooperation are key in shaping the structure and evolution of microbial communities. Our study on Bacillus subtilis, a model for microbial social interactions,...

Regulatory rewiring drives intraspecies competition in Bacillus subtilis
#SupressorMutations #RapP and #ComP

#PLOSGenetics from @bacteriacities.bsky.social

journals.plos.org/plosgenetics...

🔎 Spotted in #PLOSGenetics!
Researchers from South China Agricultural University, among others, used DSHB's anti-MyHC [B103] mAb in a study on the role of m⁶A RNA methylation in regulating circular RNA activity during chicken myogenesis.

📄 tinyurl.com/3ur2kt94

🧫 dshb.biology.uiowa.edu/B103

Parallel regulatory circuits orchestrate biofilm formation in response to c-di-GMP levels and growth phase Author summary Biofilm formation enhances the environmental fitness of bacteria and relies on the second messenger c-di-GMP; the cellular levels of c-di-GMP are controlled by diguanylate cyclases (DGC...

Parallel regulatory circuits orchestrate biofilm formation in response to c-di-GMP levels and growth phase
[in Vibrio cholerae]

#PLOSgenetics from Fitnat Yildiz
with David Grainger

journals.plos.org/plosgenetics...

Phenotype to genotype: A new and rapid approach using whole-genome sequencing. #WGS #PhenotypeToGenotype #PLOSgenetics
journals.plos.org/plosgenetics...

Phenotype to genotype: A new and rapid approach using whole-genome sequencing Author summary Forward genetic mutagenesis screening is an unbiased approach for the identification of mutations linked to a phenotype of interest. While this approach can be a powerful tool for uncov...

Check it out in #PLOSGenetics: journals.plos.org/plosgenetics...

DNA duplication in Burkholderia thailandensis induces biofilm formation by activating a two-component regulatory system

-in #PLoSGenetics

journals.plos.org/plosgenetics...

I'm excited to share our latest work, now out in #PLOSGenetics! If you feel limited by linear models in TWAS or PWAS but don't think you can switch to nonlinear methods due to a lack of individual-level phenotype data, this paper is for you.

🔗 journals.plos.org/plosgenetics...

Thread below 👇 (1/6)

Quantitative analysis of relationship between mutation rate and speed of adaptation under antibiotic exposure in Escherichia coli Author summary Bacteria evolve to survive in changing environments, but how does the rate of genetic mutations influence their ability to adapt, especially to antibiotics? In this study, we explored h...

Quantitative analysis of relationship between mutation rate and speed of adaptation under antibiotic exposure in Escherichia coli

#PLoSGenetics

journals.plos.org/plosgenetics...

Quantitative analysis of relationship between mutation rate and speed of adaptation under antibiotic exposure in Escherichia coli Author summary Bacteria evolve to survive in changing environments, but how does the rate of genetic mutations influence their ability to adapt, especially to antibiotics? In this study, we explored h...

Quantitative analysis of relationship between mutation rate and speed of adaptation under antibiotic exposure in Escherichia coli

#PLoSGenetics

journals.plos.org/plosgenetics...

Fission yeast Caprin protein is required for efficient heterochromatin establishment Author summary Regulation and stability of eukaryotic genomes is dependent on the assembly of certain regions into a distinct, repressive form of chromatin termed heterochromatin. How pre-existing het...

Published at #PLOSGenetics @plos.org
>> doi.org/10.1371/jour...

Quantitative analysis of relationship between mutation rate and speed of adaptation under antibiotic exposure in Escherichia coli Author summary Bacteria evolve to survive in changing environments, but how does the rate of genetic mutations influence their ability to adapt, especially to antibiotics? In this study, we explored h...

Quantitative analysis of relationship between mutation rate and speed of adaptation under antibiotic exposure in Escherichia coli

-in #PLoSGenetics

journals.plos.org/plosgenetics...

Mutations in the Staphylococcus aureus Global Regulator CodY confer tolerance to an interspecies redox-active antimicrobial Author summary The pathogenic bacterium Staphylococcus aureus frequently co-infects patients with another pathogen, Pseudomonas aeruginosa, and the simultaneous presence of both is associated with wor...

#CodY in #PLoSGenetics- a role in interspecies #redox active #antimicrobial #tolerance in #Staphylococcus- #Pseudomonas interactions: dx.plos.org/10.1371/jour...

Minimization of the Bacillus subtilis divisome suggests FtsZ and SepF can form an active Z-ring, and reveals the amino acid transporter BraB as a new cell division influencing factor Author summary One of the key aims of synthetic biology is the construction of a minimal cell. Since bacteria are the simplest life forms, they are the preferred blueprint for such a cell. However, a ...

Minimization of the Bacillus subtilis divisome suggests FtsZ and SepF can form an active Z-ring, and reveals the amino acid transporter BraB as a new cell division influencing factor

#PLoSGenetics from Leendert Hamoen

journals.plos.org/plosgenetics...

Capturing Heterogeneity in Gene Expression Studies by Surrogate Variable Analysis (R::sva)

Control the impact of Unmeasured/Unmodeled factors

"to disentangle correlated and overlapping differential expression signals"

Dr. John Storey lab #PLOSGenetics 2009
journals.plos.org/plosgenetics...

#PLOSGenetics: Onwards! Open science and the (PLOS) genetics community dx.plos.org/10.1371/jour...
To us, one of the most important qualities of PLOS Genetics is that it is, at its core, a community journal.

#SpatialTranscriptomics Visium
Human embryonic heart valves
Carnegie stage 16 & 19 female

Novel aortic valve markers
LINC00632 PDLIM3 FLRT2 MXRA8 RBP1

Defects of aortic valvulogenesis in RBP1 (Retinol Binding Protein 1) KO🐭

#PLOSGenetics 2023
journals.plos.org/plosgenetics...

TGF-β ligand cross-subfamily interactions in the response of Caenorhabditis elegans to a bacterial pathogen Author summary The first line of defense upon exposure to a pathogen consists of innate immunity, which includes barrier functions and cell-cell communication. These cell-cell communication signaling ...

The final formatted version of our paper on TGF-β ligands in host-pathogen response is now out in #PLOSGenetics ! This work supports the existence of cross-subfamily heterodimers, a hypothesis we plan to test next ~~~
dx.plos.org/10.1371/jour...

A general passion

Really happy to see Aimee Dudley taking the reins at #PLOSGenetics. I hope Greg Barsh and I kept it in good shape for her to do exciting things under the “by working scientists, for working scientists” banner.
dx.plos.org/10.1371/jour...

Two distinct regulatory systems control pulcherrimin biosynthesis in Bacillus subtilis

#PLOSGenetics

journals.plos.org/plosgenetics...

SHE as a negative modulator of ABL kinase & Aortic size #Zebrafish

SHE crispant🐟->
⏫ABL-CRKL-Claudin5a
⏫Cdc42-PAK4 (likely)->
⏫#EndothelialCell Proliferation+#Lumenogenesis

Love to see this #PLOSGenetics ver from preprint🥳

Saulius Sumanas lab 2024
journals.plos.org/plosgenetics...

Excited to see (and share) the final version of this work with @bryancquach! From the labs of @WilliamValdar, Ivan Rusyn, and Terry Furey.

#PLOSGenetics: Integrative QTL analysis of gene expression and chromatin accessibility identifies multi-tissu ...

dx.plos.org/10.1371/journa…

#PLOSGenetics: Human genetic variation in GLS2 is associated with development of complicated Staphylococcus aureus bacteremia http://dx.plos.org/10.1371/journal.pgen.1007667

#PLOSGenetics: Genome-wide detection of conservative site-specific recombination in bacteria

http://dx.plos.org/10.1371/journal.pgen.1007332

#PLOSGenetics: Controlled branched-chain amino acids auxotrophy in Listeria monocytogenes allows isoleucine to serve as a host signal and virulence effector

http://dx.plos.org/10.1371/journal.pgen.1007283

Novel CRISPR/Cas9 gene drive constructs reveal insights into mechanisms of resistance allele formation and drive efficiency in genetically diverse populations Author summary Gene drive systems provide a wide array of potential applications, including new strategies for the control of vector-borne diseases. For example, a functioning gene drive system could rapidly spread a genetically modified allele designed to reduce pathogen transmission throughout a vector population. The recently developed CRISPR/Cas9-based gene drive mechanism works by converting wild type alleles to drive alleles in heterozygotes via cleavage and homology-directed repair. However, resistance alleles that are unable to be converted to drive alleles can also be generated during this process, which may pose a major obstacle to the practical use of such gene drives. In our study, we developed two CRISPR/Cas9 gene drive constructs using different promoters and target sites in the model organism Drosophila melanogaster. We observed that these constructs produced resistance alleles at high rates both in the germline and post-fertilization in the embryo. Additionally, conversion efficiency and resistance allele formation rates varied substantially among genetically diverse fly lines. Overall, we conclude that new gene drive approaches will be necessary to reduce the formation of resistance alleles, particularly in genetically diverse natural populations.

#PLOSGenetics: Novel CRISPR/Cas9 gene drive constructs reveal insights into mechanisms of resistance allele f ...

What Is Speciation? Concepts and definitions of species have been debated by ...

#PLOSGenetics: What Is Speciation? http://dx.doi.org/10.1371/journal.pgen.1005860

an excellent paper about neto processing and glutamate receptors at the nmj from mihaela serpe's lab in #PLOSGenetics http://dx.doi.org/10.1371/journal.pgen.1004988

Nucleosomes Shape DNA Polymorphism and Divergence Author Summary In eukaryotic cells, the majority of DNA i...

#PLOSGenetics: Nucleosomes Shape DNA Polymorphism and Divergence http://dx.plos.org/10.1371/journal.pgen.1004457

Early Back-to-Africa Migration into the Horn of Africa Author Summary The Horn of Africa (HOA) occupies a centra...

#PLOSGenetics: Early Back-to-Africa Migration into the Horn of Africa http://dx.plos.org/10.1371/journal.pgen.1004393

Insights into the Genetic Structure and Diversity of 38 S... Author Summary Indians of South Asia has long been a popu...

#PLOSGenetics: Insights into the Genetic Structure and Diversity of 38 South Asian Indians from Deep Whole-Genome Seq http://dx.plos.org/10.1371/journal.pgen.1004377