Proud to announce our bioRxiv preprint: tinyurl.com/2k5a459h
Kudos to co-authors Chuffa, Seiva, and Reiter for demonstrating a universal regulator that restores normal cellular phase behavior—disrupting the condensates cancer cells exploit.
#PhaseSeparation #CancerResearch #Biophysics #Melatonin

Excited to share this detailed biophysical study of #PhaseSeparation in the Pyrenoid, an algal microcompartment that fixes a third of the Earth's #CO2 🌏. We combine methods across scales, in vitro 🧪 and in vivo 🦠

Thanks for the very fun collaboration! 🤗
#PlantScience 🌾 #TeamTomo 🔬 #LivingPhysics 🧬🧶

How does molecular valency shape condensate assembly and function? We used the CO2-fixing organelle in algae—the pyrenoid—to find out… 🧵

Preprint here!:
www.biorxiv.org/content/10.6...

@cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social
#Rubisco #PhaseSeparation #Condensates #Pyrenoid

#ICYMI:
“The insights of [#LaskerAward winners] Görlich & McKnight are being harnessed in medical research.” --Tanja Mittag on how low-complexity domains of proteins impact human health 🧪

#Lasker2025 #phaseseparation #biophysics
MPI for Multidisciplinary Sciences
NEJM.org
https://ow.ly/Fbmy50XUcWn

🎄Our last issue for 2025 is out!
Cover: human trunk development model

🔬 #EmbryoModels #proteostasis #tRNA #ferroptosis #trogocytosis #mechanobiology #chromatin #TranscriptionFactor #epigenetics #ExtracellularVesicles #hematopoiesis #cancer #PhaseSeparation & more!
www.nature.com/ncb/volumes/...

Captured a very obvious phase separation and fusion process (nuclear localization of tobacco cells)
#LLPS #phaseseparation

[BioRxiv] RNA modulates FUS condensate assembly, dynamics, and aggregation through diverse molecular contacts

https://doi.org/10.64898/2025.12.13.694118

#neurodegeneration #biorxiv #phaseseparation #RNAbindingprotein #aggregate

[BioRxiv] Phase Behavior of TDP-43 and hnRNP H1: From Soluble and Aggregated States to Liquid-Like Droplets

https://doi.org/10.1101/2025.11.12.687994

#neurodegeneration #biorxiv #phaseseparation #aggregate

Grateful to present at the BMB Research Colloquium @ Johns Hopkins!
Shared our work on how transient PAR signals trigger sustained FUS condensation — revealing molecular hysteresis in DNA repair foci.
Always excited to share science in person.
#BiomolecularCondensates #DNARepair #PhaseSeparation

Making sense of low-complexity domains: The 2025 Lasker Basic Science Award | PNAS Up to 10 to 20% of the proteome contains regions with much lower amino acid diversity than would be expected by chance. This year’s Lasker Basic Sc...

“The work of McKnight and Görlich is a testament to curiosity-driven research.”
#ICYMI: William G. Kaelin Jr writes about this year’s Basic Research #LaskerAward winners in the @pnas.org #Lasker2025 collection. 🧪
www.pnas.org/doi/10.1073/...
@mpi-nat.bsky.social
#phaseseparation #biophysics

[BioRxiv] Phase separation as a concentrating mechanism of protein/peptide hormones for the secretory granule storage

https://doi.org/10.64898/2025.12.05.692483

#neurodegeneration #biorxiv #phaseseparation #amyloid

I’ll be presenting at this week’s BMB Research Colloquium on how PAR triggers sustained FUS condensation (hysteresis) in DNA repair.
📍 JHSPH W3030
🕥 Thu, Dec 11 | 10:30 AM
All welcome!
Poster credit: Maranda
#DNARepair #PhaseSeparation #BiomolecularCondensates #PAR #FUS #Science

🫣

#LLPS #Droplets #phaseseparation

I've had to write about #PhaseSeparation atleast four times now, but I'll be damned if I can make heads or tales of this topic. @philipcball.bsky.social FYI might want to keep an eye on Andrea's thread.

🌟G&D SPOTLIGHT 🌟

REVIEW: RNA granules: functional compartments or incidental condensates?
By Andrea Putnam, Laura Thomas and Geraldine Seydoux
➡️ https://genesdev.cshlp.org/content/37/9-10/354.abstract

#RNAmetabolism #RNAgranules #phaseseparation #RiboNucleoProteins #condensates

Low-complexity domains: structures & functions The 2025 Albert Lasker Basic Medical Research Award honors Dirk Görlich and Steven McKnight for discoveries that exposed the structures and functions of low-complexity domains within protein…

Why are low-complexity domains (LCDs) in proteins important?
#LaskerAward winners Dirk Görlich & Steven McKnight showed that they are critical for intracellular transport and organization. #Lasker2025 🧪
#structuralbiology #phaseseparation
@mpi-nat.bsky.social

Ca2+-driven PDIA6 biomolecular condensation ensures proinsulin folding - Nature Cell Biology Lee et al. show that Ca²⁺ triggers condensates enriched with PDIA6, an ER-resident disulfide isomerase and chaperone, along with other protein disulfide isomerase family proteins and some chaperones t...

☕Lee et al. show that Ca²⁺ triggers condensates enriched with PDIA6, an ER-resident disulfide isomerase and chaperone, along with other protein disulfide isomerase family proteins and some chaperones that in turn enhance folding of #proinsulin. #PhaseSeparation
bit.ly/44mX1bW

🌟G&D SPOTLIGHT 🌟

RESEARCH PAPER: Nuclear mRNPs are compact particles packaged with a network of proteins promoting RNA–RNA interactions
By Bonneau et al. and Elena Conti
➡️ genesdev.cshlp.org/content/37/11-12/505.abs...

#ribonucleoproteins #granules #RNAinteractions #IDR #phaseseparation

Online now - the Review " #Autophagy regulation by phase separation, avidity, and wetting" from @wilflinglab.bsky.social, @kraftlabfr.bsky.social and co.

#BiomolecularCondensates #PhaseSeparation #Wetting #Avidity #Theraeputics

Read it here: authors.elsevier.com/sd/article/S...

👋Our November issue's out!
Cover: Electrostatic forces in condensates

👉🏼Comments: #DEI; #statistics #CellBiology; german #StemCell network
🔬 #ferroptosis #mitochondria #PhaseSeparation #EmbryoModels #transcriptomics #chromatin #cancer #TransposableElements & more!
www.nature.com/ncb/volumes/...

“The Enigma of Protein Domains of Low-Sequence Complexity”
#LaskerAward winner S. McKnight writes about “these ugly duckling proteins” that turned out to be vital for cellular function. 🧪
#RNAbiology #phaseseparation #Lasker2025 #UTSWMedicalCenter #JAMA
ow.ly/klVz50WY5Hf

Biomolecular condensates: molecular structure, biological functions, diseases, and therapeutic targets - Molecular Biomedicine Cells constantly encounter environmental and physiological fluctuations that challenge homeostasis and threaten viability. In response to these cues, specific proteins and nucleic acids engage in mult...

#Review
Biomolecular condensates: molecular structure, biological functions, diseases, and therapeutic targets doi.org/10.1186/s435...

#PhaseSeparation #BiomolecularCondensate #NuclearSpeckle #Paraspeckle #StressGranule

Phase-separated NDF−FACT condensates facilitate transcription elongation on chromatin - Nature Cell Biology Li, Burgos-Bravo and colleagues report that NDF phase separation regulates FACT condensation, which enhances transcription by generating a localized biochemical environment that promotes nucleosome di...

☕Li, Burgos-Bravo et al. report that NDF #PhaseSeparation regulates FACT condensation, which enhances transcription by generating a localized biochemical environment that promotes nucleosome disassembly while preserving #chromatin integrity by retaining histones.
bit.ly/3JwVetO

Paralogs with distinct phase behaviors broaden the in vivo stress response range of condensates Paralogs are widespread, but their physiological roles are often masked by redundancy. H-NS, a nucleoid-associated protein in Gram-negative bacteria, typically coexists with paralogs such as StpA, who...

Bacteria use paralog teamwork to survive stress.
H-NS forms fluid condensates; its partner StpA forms rigid fibrils. Together, they balance flexibility & stability to keep genes properly regulated under pressure.
#PhaseSeparation #Bacteria
www.biorxiv.org/content/10.1...

Epitranscriptomics #PhaseSeparation

m6A-modified #EnhancerRNA & YTHDC1-BRD4 assemble transcriptional condensate

MINT-seq detects >3-fold more m6A peaks than MeRIP-seq

Stringtie annotate eRNAs & promoter upstream antisense RNAs, 24% & 48% are m6A+ (MCF7)

#MolCell 2021
www.cell.com/molecular-ce...

🎃Our October issue's out!
Cover: mechanoresponsive #microtubules

👉🏼Reviews: #CellularStress ; #NuclearPoreComplex & #mechanobiology
🔬 #StemCells #senescence #cancer #EndoplasmicReticulum #phagocytosis #pyroptosis #autophagy #PhaseSeparation #AMPK #chromatin & more!
www.nature.com/ncb/volumes/...

The KEY🔑 to regulating Rubisco phase separation in the Chlamydomonas algal pyrenoid. Beautiful work by Shan He, @mjonikas.bsky.social, and friends. #PhaseSeparation #CO2 #PlantScience 🧪 🧶🧬 🌾 🌍

🚨 Publication🚨: ACS Nano: @laynefrechette.bsky.social
and @n4rens.bsky.social use Langevin dynamics simulations to demonstrate how self-assembly of empty #ViralCapsidAssembly is modulated by liquid compartments formed by #PhaseSeparation. Check it out: pubs.acs.org/doi/10.1021/... #BrandeisMRSEC

🚨 Publication Alert 🚨
Congratulations to Weixiang Chen from @waltherlab.bsky.social Andreas Walther Lab on their recent publication in #NatureCommunications! 🎉👏
#SyntheticNuclearArchitectures #TranscriptionalCondensates #DNAProtonucleus #BiomolecularCondensates #PhaseSeparation #SFB1551 #UniMainz

We also observed that PSII concentration was uniform throughout each granum, irrespective of distance from the margin. Thus, we propose a simple two-domain model of thylakoid organization in green algae and plants. And yes, we speculate about domain #PhaseSeparation. I await your hate mail 📬🤗

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