Prime-SGE: #saturation #mutagenesis via #primeediting
3825 epegRNAs → 1220 mutations in 7 cancer genes (EGFR, KRAS, BRAF, MET, MEK1, AKT, RIT1)
EGFR inhibitor screen: 17-37 resistance variants/drug. KRAS G12 = pan-resistance. C797S only in covalent inhibitors.
In vivo validated ✓
#CancerResearch

SHOT 2.0: CAST-based platform for programmable baculovirus engineering

→ ≥14 kb cargo, dual-loci integration, stable transgene retention (ODVe56 > v-cath)
→ All-in-one Bac-PE5max: 85.6% in HEK293T, 31.3% in iPSCs

📄 pmc.ncbi.nlm.nih.gov/articles/PMC...

#PrimeEditing #Baculovirus #CRISPR

Adolescente de Columbia Británica se convierte en la primera persona Curada mediante Edición Genética PRIME | Ciencia y Cultura | CIBERED Prime editing logra primera cura de enfermedad genética rara. Ty Sperle supera enfermedad granulomatosa. Hito en medicina de precisión. ¡Descubre el avance!

🧬 Adolescente de Columbia Británica se convierte en la primera persona Curada mediante Edición Genética PRIME cibered.com/tecnologia/c...

#EdicionGenetica #PrimeEditing #InnovacionMedica #Cibered

Deaminet 2026 - Harris Lab

Anyone attending #Deaminet2026 on January 21-23? Looking forward to catching up on the latest developments in #baseediting and #primeediting!

labs.uthscsa.edu/rsh/deaminet...

@addgene.bsky.social will be sponsoring poster prizes!

#CRISPR #geneediting #GeneAndCellTherapy #CellAndGeneTherapy

proPE splits tasks between two guides: engRNA nicks the DNA (exposing the non-target strand); tpgRNA carries PBS+RTT and binds nearby with a truncated spacer, delivering the template without cutting.
See schematics. #CRISPR #primeediting

ProPE expands the prime editing window and enhances gene editing efficiency where prime editing is inefficient - Nature Catalysis Prime editing is a CRISPR methodology whose efficiency declines with distance from the target sequence. Here the authors demonstrate prime editing with prolonged editing window, proPE, which extends t...

Delighted to share our new method proPE (prime editing with prolonged editing window) is out in Nature Catalysis: www.nature.com/articles/s41...
Kudos to first author Sarah Laura Krausz and all co-authors at HUN-REN TTK & HUN-REN SZBK! #CRISPR #primeediting

MIT Reduces Prime-Editing Errors Ten-Fold for Gene-Therapy Accuracy

MIT researchers unveiled vPE, a prime‑editing version that lowers error rates about ten‑fold, cutting mistakes to roughly 1 in 101 edits in standard mode and 1 in 543 in high‑precision mode. getnews.me/mit-reduces-prime-editin... #primeediting #mit

A more precise way to edit the genome MIT researchers have dramatically lowered the error rate of prime editing, a genome-editing technique based on CRISPR. This advance could make it easier to develop gene therapy treatments for a variet...

A more precise way to edit the #Genome.

#MIT researchers have dramatically lowered the error rate of #PrimeEditing, a technique that holds potential for treating many #Genetic #Disorders. news.mit.edu/2025/more-pr...

Engineered prime editors with minimal genomic errors - Nature Engineered prime editor systems with reduced occurrences of unwanted insertions or deletions during genome editing are developed.

Genome editors like prime editors can rewrite the genetic code – but sometimes they incorporate undesired errors. Today in Nature (@nature.com), we report how we engineered prime editors with strikingly low errors.
www.nature.com/articles/s41...
#GenomeEditing #CRISPR #PrimeEditing #genetherapy 🧪

Coming up in September!
➡️Christina Kajba @ckajba.bsky.social & Michael Herger @michaelherger.bsky.social — pooled prime editing screens in haploid human cells
➡️Jon Acosta — multiplexed in vivo base editing screens
#GeneticVariants #PrimeEditing
ℹ️https://www.varianteffect.org/seminar-series/

Prime editing treats childhood brain disease in mice Scientists use a precise form of gene editing called prime editing to correct the most common genetic mutations that cause alternating hemiplegia of childhood, a rare and severe neurological disorder ...

Partnership between David Liu, @jacksonlab.bsky.social, and rare disease group RARE Hope leads to first use of prime editing for a brain disease that reduced symptoms, extended lifespan in mice, offering hope for other rare genetic diseases. #Science #PrimeEditing

Per la prima volta, la tecnica di #primeediting è stata utilizzata su un paziente affetto da malattia granulomatosa cronica.

📈 I risultati preliminari sono buoni, ma la sperimentazione potrebbe non proseguire.

Tutte le info:
👉 www.osservatorioterapieavanzate.it/terapie-avan...

The next session is on Genetic Technologies, and the first speaker is Prof. Sabine Fuchs (from @umcutrecht.bsky.social). She will talk about prime editing for inherited metabolic diseases. #NVGCT2025 #metabolicdiseases #primeediting #liver

Breaking...2nd Patient with Chronic Granulomatous Disease Successfully Treated with #PrimeEditing (blog): rnaitherapeutics.blogspot.com/2025/06/2nd-...

$PRME $BEAM #CRISPR

Prime Editing: The Next Frontier in Genetic Medicine Prime editing is an advanced gene editing technology that enables precise DNA corrections without introducing double-stranded breaks, significantly reducing off-target effects. This innovation holds p...

Prime Editing: The Next Frontier in Genetic Medicine www.news-medical.net/health/Prime... #PrimeEditing #GeneEditing #Genome #GeneticMedicine #CRISPR #PersonalizedMedicine #Biotechnology #Genetics #DNARepair

World first: ultra-powerful CRISPR treatment trialled in a person Immune-cell function improved in a teenager whose DNA was altered using prime editing.

First patient receiving cells edited with prime editing, to treat chronic granulomatous disease! 💪🏻

#CRISPR #PrimeEditing

First Curative Correction of a Deletion Mutation by Prime Editing Another week, another disease cured by CRISPR technology.   Today, Prime Medicine revealed that a young man with autosomal recessive chroni...

First Curative Correction of a Deletion Mutation by #PrimeEditing (blog): rnaitherapeutics.blogspot.com/2025/05/firs...
$PRME $BEAM #CRISPR

TBC1D30 regulates proinsulin and insulin secretion and is the target of a genomic association signal for proinsulin - Diabetologia Aims/hypothesis Components of the insulin processing and secretion pathways remain incompletely understood. Here, we examined a genome-wide association study (GWAS) signal for plasma proinsulin levels...

TBC1D30 sequence & expression changes impact proinsulin & insulin secretion, linking this putative RabGAP to GWAS signal for proinsulin levels. Study suggests role for TBC1D30 in proinsulin secretion & diabetes risk. #T2D #Proinsulin #GWAS #PrimeEditing link.springer.com/article/10.1...

🧬 CRISPR Meets Probiotics🦠

What if we could engineer probiotics with precision to fight disease and promote animal and plant health?

Read here-

🔗https://journals.asm.org/doi/10.1128/aem.00031-25

#CRISPR #PrimeEditing #MicrobiomeEngineering #Probiotics #SyntheticBiology #PrecisionMedicine

Scientists map cancer mutations in EGFR gene, revealing drug resistance paths Researchers used base and prime editing to uncover new EGFR gene mutations that affect cancer progression and drug resistance, paving the way for personalized treatment strategies.

Scientists map cancer mutations in EGFR gene, revealing drug resistance paths 🔬🧬🧪 www.news-medical.net/news/2024111... #Cancer #GeneEditing #EGFR #PrecisionMedicine #DrugResistance #CancerProgression #BaseEditing #PrimeEditing #PersonalizedTreatment @naturebiotech.bsky.social

Saturation profiling of drug-resistant genetic variants using prime editing - Nature Biotechnology PEER-seq characterizes the drug resistance profiles of thousands of genetic variants.

Saturation profiling of drug-resistant genetic variants using prime editing. #GeneticVariants #SaturationProfiling #PrimeEditing #NatureBiotechnology
www.nature.com/articles/s41...

Multimodal scanning of genetic variants with base and prime editing - Nature Biotechnology Thousands of rare oncogene variants are evaluated using multimodal gene editing screens.

Multimodal scanning of genetic variants with base and prime editing. #GeneticVariants #PrimeEditing #NatureBiotechnology
www.nature.com/articles/s41...

CRISPR technologies for genome, epigenome & transcriptome editing

Dazzling ever-expanding Cas-verse😎
#BaseEditing
#PrimeEditing
Large insertions/deletion kb
Transient/Stable transcriptional activation/inhibition
RNA degradation/editing/methylation🤠

#NatRevMCB 2024
www.nature.com/articles/s41...