Next month, we will present multiple posters and oral presentations during the @ESCMIDGlobal, but 2 are among the top 2% of highest rated submissions.
We see you in Munich! #surveillance #collaboration #RIVM #SSI #genomics #plasmids #outbreak #ESCMID #ESGMAP #CPEsurveillanceStudyGroup #ECDC
Celebrating one year of incredible achievements! 🥳 Feeling incredibly humbled and proud of everything we've accomplished. This is just the beginning...
#ESGMAP #Plasmids #MGEs #Celebration #Milestone #Journey
Mathematical modeling predicts that CRISPR-Cas has a greater potential to benefit its plasmid in defence than in offense. Structure of the mathematical model when a CRISPR-Cas plasmid is the resident (left) or the invader plasmid (right). The authors consider seven distinct cell types: C (CRISPR-Cas bearing) and T (TA-bearing) plasmids reside in host cells; CT denotes a C cell that has been invaded by a T plasmid and vice versa; Cr* and Ci* cells have undergone segregational loss of a T plasmid, and the fate of PSK is not yet resolved; PSK cells are dead cells after post-segregational killing. When the plasmids co-reside, they are subject to basic biological parameter s (segregational loss). CRISPR-Cas and TA alter parameter s by their own modes of action x and y, respectively. Parameter f describes the positive effect PSK cells have on growth of nearby cells, where fr describes the benefit to cells containing the previously resident plasmid. All parameters adopt distinct values when their respective plasmids are resident (r) or invasive (i).
Plasmids use immune systems like #CRISPR-Cas to compete with other #plasmids, but do these systems confer an advantage? @davvi36.bsky.social &co show that CRISPR-Cas benefits resident plasmids but is constrained by toxin-antitoxin systems after horizontal transfer @plosbiology.org 🧪 plos.io/4qNbdDt
Mathematical modeling predicts that CRISPR-Cas has a greater potential to benefit its plasmid in defence than in offense. Structure of the mathematical model when a CRISPR-Cas plasmid is the resident (left) or the invader plasmid (right). The authors consider seven distinct cell types: C (CRISPR-Cas bearing) and T (TA-bearing) plasmids reside in host cells; CT denotes a C cell that has been invaded by a T plasmid and vice versa; Cr* and Ci* cells have undergone segregational loss of a T plasmid, and the fate of PSK is not yet resolved; PSK cells are dead cells after post-segregational killing. When the plasmids co-reside, they are subject to basic biological parameter s (segregational loss). CRISPR-Cas and TA alter parameter s by their own modes of action x and y, respectively. Parameter f describes the positive effect PSK cells have on growth of nearby cells, where fr describes the benefit to cells containing the previously resident plasmid. All parameters adopt distinct values when their respective plasmids are resident (r) or invasive (i).
Plasmids use immune systems like #CRISPR-Cas to compete with other #plasmids, but do these systems confer an advantage? @davvi36.bsky.social &co show that CRISPR-Cas benefits resident plasmids but is constrained by toxin-antitoxin systems after horizontal transfer @plosbiology.org 🧪 plos.io/4qNbdDt
Mathematical modeling predicts that CRISPR-Cas has a greater potential to benefit its plasmid in defence than in offense. Structure of the mathematical model when a CRISPR-Cas plasmid is the resident (left) or the invader plasmid (right). The authors consider seven distinct cell types: C (CRISPR-Cas bearing) and T (TA-bearing) plasmids reside in host cells; CT denotes a C cell that has been invaded by a T plasmid and vice versa; Cr* and Ci* cells have undergone segregational loss of a T plasmid, and the fate of PSK is not yet resolved; PSK cells are dead cells after post-segregational killing. When the plasmids co-reside, they are subject to basic biological parameter s (segregational loss). CRISPR-Cas and TA alter parameter s by their own modes of action x and y, respectively. Parameter f describes the positive effect PSK cells have on growth of nearby cells, where fr describes the benefit to cells containing the previously resident plasmid. All parameters adopt distinct values when their respective plasmids are resident (r) or invasive (i).
Plasmids use immune systems like #CRISPR-Cas to compete with other #plasmids, but do these systems confer an advantage? @davvi36.bsky.social &co show that CRISPR-Cas benefits resident plasmids but is constrained by toxin-antitoxin systems after horizontal transfer @plosbiology.org 🧪 plos.io/4qNbdDt
Build better #plasmids with confidence. Learn how #CodonOptimization and smart preparation strategies reduce errors and improve #gene design. Register today! hubs.ly/Q043zbn80
#Bacterial hitchhikers can give their hosts super strength ...
| #microbes | #bacteria | #infections | #plasmids | #pili | Via @sciencex.bsky.social
Our results suggest plasmids are active ecological agents that clear their own niche by eliminating non-permissive competitors. 🦠❌🤝🦠
🔗 Full paper here: www.biorxiv.org/content/10.6...
#Microbiology #SynBio #Plasmids #CRISPR (6/6)
Our results suggest plasmids are active ecological agents that clear their own niche by eliminating non-permissive competitors. 🦠❌🤝🦠
🔗 Full paper here: www.biorxiv.org/content/10.6...
#Microbiology #SynBio #Plasmids #CRISPR (6/6)
🧬 Plasmids are essential genetic tools 🔬, enabling gene cloning, recombinant DNA formation, and protein production in modern biotechnology 🧪.
#Plasmids #GeneCloning #RecombinantDNA #MolecularBiology #Biotechnology #GeneticEngineering #LifeScienceStudents #Bioinformatics #LabTechniques #BioResire
Thrilled to be starting my postdoc @unibirmingham.bsky.social.
Working with @bucknerlab.bsky.social to explore how biofilm growth influences the evolution of plasmid-mediated AMR and hyper-virulence in Klebsiella pneumoniae. Big questions, exciting times.
#AMR #Biofilms #plasmids #microsky
🧬 Plasmids are powerful genetic tools 🔬, enabling gene cloning, recombinant DNA formation, and protein production in modern biotechnology 🧪.
#Plasmids #GeneCloning #RecombinantDNA #Biotechnology #MolecularBiology #GeneticEngineering #LifeScienceStudents #Bioinformatics #LabTechniques #BioResire
But in these New Zealand cases, the OXA-48 gene wasn’t on a massive #plasmid
It was carried on a tiny plasmid ~8,000 DNA letters long
That’s quite small
#Genetics #DNA #Plasmids #Science #Easyfig
🖥️🧬💻
#AcademicSky
#MicroSky
#IDSky
🧪🧫🦠
🧵BIG PROBLEMS can hide in small #Plasmids
📌Integration of blaOXA-48 into a Col156 plasmid drove a carbapenem-resistant Escherichia coli ST131 outbreak in New Zealand: Global genomic evidence for the gene’s multilayered dissemination
doi.org/10.1016/j.dr...
🖥️🧬💻
#AcademicSky
#MicroSky
#IDSky
🧪🧫🦠
Fig. 1 from https://www.nature.com/articles/s41467-025-66874-7. A) shows a map of the world, with the distribution of IMP variants. B) shows a stacked column graph of IMP variants over time (1996-2023). C) shows the number of IMP-positive genomes over time, by geographic region.
(2/7) Read this if:
- You are interested in #carbapenem resistance or #AMR
- Love #plasmids or #MGE
- Work on #OneHealth
- Want to learn about the global distribution of antimicrobial resistance and its spread
- Enjoy #DataVis
Evolved plasmid-carrying clones with high copy number contain a mix of full-length and streamlined plasmids. Left: summary of the initial experimental evolution design and nomenclature used for the evolved plasmid-carrying clones. Host strains had either wildtype (w) or increased (m) mutation rate. Treatment indicates the % of plasmid-free hosts added at each passage during evolution. Right: coverage map of evolved clones. Relative coverage of sequencing reads is shown for all clones across R1 sequence length (only the first 50 kb are shown). The AMR region and copies of IS1 are shown against the ancestral R1 (“anc” treatment) coverage map.
Conjugative plasmids help spread AMR genes. @andrewmatthews.bsky.social @sonjalehtinen.bsky.social & @tatianadimitriu.bsky.social show that #AMR loss in evolving populations involves streamlined #plasmids that gain a transmission advantage by deleting AMR genes @plosbiology.org 🧪 plos.io/48NM0BL
We are pleased to share our fourth and final newsletter of 2025 (tinyurl.com/bdhf7uhm). We are bringing you summaries of the year, activities to look forward to, and we are introducing a Slack workspace to facilitate idea sharing and collaboration. #ESCMID #ESGMAP #plasmids #newsletter #AMR #webinar
Evolved plasmid-carrying clones with high copy number contain a mix of full-length and streamlined plasmids. Left: summary of the initial experimental evolution design and nomenclature used for the evolved plasmid-carrying clones. Host strains had either wildtype (w) or increased (m) mutation rate. Treatment indicates the % of plasmid-free hosts added at each passage during evolution. Right: coverage map of evolved clones. Relative coverage of sequencing reads is shown for all clones across R1 sequence length (only the first 50 kb are shown). The AMR region and copies of IS1 are shown against the ancestral R1 (“anc” treatment) coverage map.
Conjugative plasmids help spread AMR genes. @andrewmatthews.bsky.social @sonjalehtinen.bsky.social & @tatianadimitriu.bsky.social show that #AMR loss in evolving populations involves streamlined #plasmids that gain a transmission advantage by deleting AMR genes @plosbiology.org 🧪 plos.io/48NM0BL
Evolved plasmid-carrying clones with high copy number contain a mix of full-length and streamlined plasmids. Left: summary of the initial experimental evolution design and nomenclature used for the evolved plasmid-carrying clones. Host strains had either wildtype (w) or increased (m) mutation rate. Treatment indicates the % of plasmid-free hosts added at each passage during evolution. Right: coverage map of evolved clones. Relative coverage of sequencing reads is shown for all clones across R1 sequence length (only the first 50 kb are shown). The AMR region and copies of IS1 are shown against the ancestral R1 (“anc” treatment) coverage map.
Conjugative plasmids help spread AMR genes. @andrewmatthews.bsky.social @sonjalehtinen.bsky.social & @tatianadimitriu.bsky.social show that #AMR loss in evolving populations involves streamlined #plasmids that gain a transmission advantage by deleting AMR genes @plosbiology.org 🧪 plos.io/48NM0BL
🧬 How do we evaluate structural variations in plasmids?
Find out in the final ESGMAP webinar in 2025.
💥Dr. Daria Frolova presents "Studying plasmid relatedness and evolution through structural variation"
📅15 December, 12:00 CET
🔗Registration required (link in comments)
#ESCMID #ESGMAP #plasmids
#evolution
#plasmids
Great paper (imo) beginning with "the genetic history of the Murray collection, a rare assemblage of bacterial samples from 1917 to 1954..."
Professor Murray's & his collection's history tout court
www.microbiologyresearch.org/sotsog/egd-m...
The collection of the journal BMC Microbiology "Antimicrobial Resistance" where I will serve as a guest editor is OPEN for research article submissions!
#AMR #plasmids #spread #genomics #RIVM #editor #BMCmicrobiology #Nanopore #Illumina #sequencing
link.springer.com/collections/...
The next five videos from our #IMMEM XIV poster presentations are available on our YouTube channel and cover topics like #plasmids, #genomics, #sequencing and more. Stay tuned for more videos every Wednesday!
youtube.com/playlist
Remember to join our @escmid.bsky.social study group webinar tomorrow.
Registration required.
#ESCMID #ESGMAP #plasmids #webinar #series
Subverting #plasmids to combat #antibiotic resistance ...
| #antibioticresistance | #bacteria | #evolution | #antimicrobial | By@harvardmed.bsky.social via @sciencex.bsky.social
Genomics isn’t just solving #Outbreaks, it’s stopping them before they escalate
HOT take from @natrevmicro.nature.com: If you only sequence #MDR outbreaks, you’re already late. Consider susceptible strains & #Plasmids
#GenomicSurveillance #InfectionControl
🖥️🧬💻
#AcademicSky
#MicroSky
#IDSky
🧪🧫🦠
We are pleased to share our third newsletter (www.escmid.org/fileadmin/es...), with upcoming scientific meetings and webinars with experts in the field.
Enjoy the read, and stay tuned for the next one.
#ESCMID #ESGMAP #plasmids #newsletter #AMR #webinars
Understand bacterial genetics and gene transfer mechanisms driving diversity and resistance. #BacterialGenetics #Microbiology #GeneTransfer #Transformation #Transduction #Conjugation #Transposition #Plasmids #Bacteriophage #AntibioticResistance #USMLE #MedicalEducation #ClinicalMicrobiology #Genetic
🧬 #HelicobacterPylori carries plasmids that may aid its survival in hostile environments. This #FEMSMicrobiolLett reveals plasmid-driven adaptation and a novel toxin-antitoxin system crucial for its persistence. 🔍 Read more: buff.ly/h56fzgw #Microbiology #Plasmids
