⭐ #NARBreakthrough! ⭐
🧬 New study uncovers how #RNAstructures control stochastic exon selection in Dscam1 genes. #Balancer RNA structures and a #multi-subunit RNA architecture coordinate spatial and temporal splicing 🔬✨
Read the full article here: doi.org/10.1093/nar/...
#AlternativeSplicing

#AlternativeSplicing analysis pipeline

MAJIQ v2 - VOILA v2 modulizer

👉Build gene splicegraph including unannotated, complex elements (e.g. de novo intron retention)
👉Quantify AS events & modules
👉Comparative analysis of GTEx v8 all 53 tissues

#NatComm 2023
www.nature.com/articles/s41...

🚨 New paper alert! 🚨

Our method paper entitled: "Semi-quantitative RT-PCR Assay for the Analysis of Alternative Splicing of Interleukin Genes" is out now on the volume "Immune Mediators in Cancer"!

🧵 Thread 👇

#AlternativeSplicing
#SpliceVariant
#Interleukins
#Cytokines

#AlternativeSplicing

Systematic profiling of AS program & splicing factors (RBPMS, RBFOX2, MBNL1/2, Quaking) in Smooth Muscle Cell

RBPMS, RBFOX2 & MBNL1/2, but not QK, are pro-contractile in PAC1 SMC

#NucAcidRes 2025
academic.oup.com/nar/article/...

This #STTT study shows that hnRNPR regulates m6A-dependent #AlternativeSplicing of Skap2, positioning it as a key regulator of #Spermiogenesis, while highlighting mEVs-SKAP2 as a promising #TherapeuticTarget.

#OpenAccess: doi.org/10.1038/s413...

College of Medicine, National Taiwan University.

Me and members of the Huang lab.

Me and Dr. Hsien-Sung Huang.

Thank you, Dr. Hsien-Sung Huang for hosting me during my stay in #Taipei & for inviting me to present my work at #NationalTaiwanUniversity. I had a wonderful time talking #organoids, #AlternativeSplicing, #RNAediting, & Rbfox 3.

Huang lab (黃憲松實驗室) share.google/Rv5o6uwhByHV...

#Taiwan
#IonChannels

Alternative Splicing: Molecular Mechanisms, Biological Functions, Diseases, and Potential Therapeutic Targets Alternative splicing (AS) expands proteomic diversity and functional complexity in eukaryotes, regulated by spliceosomal components, RNA elements, and epigenetic modifications. Dysregulated AS contri...

#Review in #MedComm
Alternative Splicing: Molecular Mechanisms, Biological Functions, Diseases, and Potential Therapeutic Targets doi.org/10.1002/mco2...

#AlternativeSplicing #BiologicalFunction #disease #MolecularMechanism #TherapeuticTarget

While reaching for cookies, we stumbled on a treat of a different kind: DIGGER 2.0 for exploring the functional role of protein interactions in #AlternativeSplicing! ☃️🍪
Now supporting the mouse interactome and the functional enrichment tool NEASE #CoSyAdventcalender

👉 exbio.wzw.tum.de/digger/

Robust Integration of Sparse Single-Cell Alternative Splicing and Gene Expression Data with SpliceVI
Isaev, K. et al.
Paper
Details
#SingleCell #AlternativeSplicing #SpliceVI

Advancing automated cell type annotation with large language models and single-cell isoform sequencing. Computational and Structural Biotechnology Journal, DOI: https://doi.org/10.1016/j.csbj.2025.11.008

🔗 Advancing automated cell type annotation with large language models and single-cell isoform sequencing. Computational and Structural Biotechnology Journal, DOI: doi.org/10.1016/j.cs...

📚 CSBJ: www.csbj.org

#LargeLanguageModels #Genomics #CellBiology #AlternativeSplicing #LLMs #SystemsBiology

KAT2A-mediated #succinylation of the #splicingfactor SRSF11 at K419 enhances #DNARepair and #radioresistance in #HCC by promoting RAD52 exon inclusion, linking #AlternativeSplicing to #GenomicStability and offering a potential therapeutic target.

#STTT: doi.org/10.1038/s413...

Schematic model depicting the role of PRMT5-mediated symmetric arginine dimethylation in regulation of TCF3 alternative splicing under normoxia (left) and hypoxia (right). Created in BioRender. Shukla, S.

PRMT5 is a #HistoneMethyltransferase upregulated in various cancer types. This study shows that PRMT5 regulates TCF3 #AlternativeSplicing under #hypoxia and that this promotes #EMT and invasion of #BreastCancer cells @plosbiology.org 🧪 plos.io/48ZbZrT

Schematic model depicting the role of PRMT5-mediated symmetric arginine dimethylation in regulation of TCF3 alternative splicing under normoxia (left) and hypoxia (right). Created in BioRender. Shukla, S.

PRMT5 is a #HistoneMethyltransferase upregulated in various cancer types. This study shows that PRMT5 regulates TCF3 #AlternativeSplicing under #hypoxia and that this promotes #EMT and invasion of #BreastCancer cells @plosbiology.org 🧪 plos.io/48ZbZrT

Schematic model depicting the role of PRMT5-mediated symmetric arginine dimethylation in regulation of TCF3 alternative splicing under normoxia (left) and hypoxia (right). Created in BioRender. Shukla, S.

PRMT5 is a #HistoneMethyltransferase upregulated in various cancer types. This study shows that PRMT5 regulates TCF3 #AlternativeSplicing under #hypoxia and that this promotes #EMT and invasion of #BreastCancer cells @plosbiology.org 🧪 plos.io/48ZbZrT

Synergy between RNA editing and alternative splicing modulates the biological properties of the voltage-gated calcium channel CaV1.3 Alternative splicing and adenosine-to-inosine (A-to-I) RNA editing are post-transcriptional mechanisms that promote proteomic diversity in the brain. The impact of RNA editing on brain physiology is u...

🚨 New paper alert! 🚨

Our paper entitled: "Synergy between RNA editing and alternative splicing modulates the biological properties of the voltage-gated calcium channel CaV1.3" is out now on Journal of Biological Chemistry!

www.jbc.org/article/S002...

#AlternativeSplicing #RNAediting

🧵 👇

Predicting the structural impact of human alternative splicing. #AlternativeSplicing #GenomeBiology
genomebiology.biomedcentral.com/articles/10....

Multiomics analyses of #SCLC #tumors reveals widespread #AlternativeSplicing and frequent #FocalAdhesionKinase splicing variants that drive aggressive disease, predict poor prognosis, & show marked sensitivity to FAK inhibitors. #medsky

#STTT #OpenAccess: doi.org/10.1038/s413...

Good morning Sky-Friends!
Our own @biochemtrix.bsky.social (and occasional poster!) is in Germany at the #Unleash consortium meeting. They are joining us here in the sky @unleash-as.bsky.social so follow along to find out about #AlternativeSplicing!

It's day 2 of the UNLEASH meeting! We are in beautiful Bernried and looking forward to hearing more about the progress our members have made unpicking the details of #AlternativeSplicing!

We are all here in sunny Munich to talk about the progress we have all made UNLEASHing the secrets of #AlternativeSplicing

Members of four different scientific groups across three different countries collaborating and sharing data.

Follow us to see our progress too!

This exciting issue includes articles on #AI and #Huanglongbing resistance, vertical farming, #reviews on genetic variation in adaptive #evolution, and on #AlternativeSplicing, along with much more research!

Read more great #PlantScience from this issue here: onlinelibrary.wiley.com/toc/17447909...

Computational workflow to analyze #AlternativeSplicing
vast-tools
Matt
ExOrthist
VastDB (vertebrate database) vastdb.crg.eu/wiki/Main_Page
PastDB (A. thaliana database) pastdb.crg.eu/wiki/About

Manuel Irimia lab #MethodMolBiol 2022
link.springer.com/protocol/10....

Our new review about RNA processing and mechanotransduction is out in Cell Reports! Link: lnkd.in/edApA4ZT. Proud of our very talented graduate student Gabrielle Bais and thankful to our institution and funding sources: UNC-Chapel Hill, NIH, NSF.
#mechanotransduction #RNA #alternativesplicing

This study reveals that TPM3P9 promotes #tumor growth in #ClearCellRenalCellCarcinoma (ccRCC) by enhancing the #alternativesplicing of oncogenic RNAs and highlights it as a potential therapeutic target in ccRCC management. #medsky

#STTT #OpenAccess: doi.org/10.1038/s413...

Presenting DIGGER 2.0: we extended our #AlternativeSplicing software DIGGER with new functionalities, including support of the mouse interactome and NEASE for #EnrichmentAnalysis! Check it out here: exbio.wzw.tum.de/digger/

DIGGER 2.0: digging into the functional impact of differential splicing on human and mouse disorders Abstract. Changes in alternative splicing between groups or conditions contribute to protein–protein interaction rewiring, a consequence often neglected in

Discover a new tool: DIGGER 2.0 enables splice-aware functional annotation of interaction network changes using RNA seq analysis via NEASE.
#VirusBioinformatics #AlternativeSplicing #InteractomeTools

📄 doi.org/10.1093/nar/...
👤EVBC: Olga Kalinina

GitHub - alihamraoui/scKenver Contribute to alihamraoui/scKenver development by creating an account on GitHub.

🛠️ Resources

All code, datasets, and reproducible pipelines are openly available: github.com/alihamraoui/...

#bioinformatics #RNAseq #singlecell #alternativesplicing #benchmark #opensource #scRNAseq #MinION

cc @fr-genomics.bsky.social @mthomaschollier.bsky.social

Single cell and spatial alternative splicing analysis with Nanopore long read sequencing. #SingleCell #scRNAseq #AlternativeSplicing #LongReads #Sequencing @nanoporetech.com @natcomms.nature.com
www.nature.com/articles/s41...

Next Up in the #LongTREC Series 📽️ : Yalan Bi!
Yalan is a LongTREC Doctoral Candidate working with Ralf Herwig at @molgen.mpg.de Yalan shares insights into her research project and her experience as part of the LongTREC network. #LongReadsTranscriptomics #AlternativeSplicing #ProteinInteraction

Alternative Splicing and CaV‐Associated Channelopathies Voltage-gated calcium channels control vital cellular processes through complex alternative splicing mechanisms, and their dysfunction contributes to neurological, cardiac, endocrine, oncogenic, and ...

📢 #PaperAlert

Review article in @WIREs_Reviews on #AlternativeSplicing, voltage-gated #calcium #channels, and #channelopathies. #IonChannels

Alternative Splicing and CaV‐Associated Channelopathies - Munyao - 2025 - WIREs RNA - Wiley Online Library wires.onlinelibrary.wiley.com/doi/10.1002/...