Left: Graphic summary of the USPPAR approach. The entire process is divided into three stages. Dissociation involves separating whole cells from culture dishes or extracting nuclei from tissues, followed by fixation. Barcoding includes converting mRNA to cDNA with initial tagging, then performing 2–3 additional rounds of barcoding through ligation. Amplification encompasses cell/nucleus lysis, high-efficiency poly(dA) extension of cDNA, preamplification, tagmentation, and final library amplification. Right: Joint UMAPs of 50,584 murine hepatic nuclei, including 12,646 nuclei from each technology: USPPAR_pH3 (pH3 lysis), USPPAR_CuC (CuC lysis), Liver Cell Atlas (LCA, 10× Chromium), and PanSci (EasySci platform). Nuclei are colored by technology (Technology)

Single-cell RNA-sequencing based on combinatorial barcoding is pricey and lacks sensitivity. USPPAR is a novel #scRNAseq approach that combines split-pool #barcoding & optimized polydeoxyadenylation, outperforming existing methods without specialized equipment @plosbiology.org 🧪 plos.io/3XUsleH

Left: Graphic summary of the USPPAR approach. The entire process is divided into three stages. Dissociation involves separating whole cells from culture dishes or extracting nuclei from tissues, followed by fixation. Barcoding includes converting mRNA to cDNA with initial tagging, then performing 2–3 additional rounds of barcoding through ligation. Amplification encompasses cell/nucleus lysis, high-efficiency poly(dA) extension of cDNA, preamplification, tagmentation, and final library amplification. Right: Joint UMAPs of 50,584 murine hepatic nuclei, including 12,646 nuclei from each technology: USPPAR_pH3 (pH3 lysis), USPPAR_CuC (CuC lysis), Liver Cell Atlas (LCA, 10× Chromium), and PanSci (EasySci platform). Nuclei are colored by technology (Technology)

Single-cell RNA-sequencing based on combinatorial barcoding is pricey and lacks sensitivity. USPPAR is a novel #scRNAseq approach that combines split-pool #barcoding & optimized polydeoxyadenylation, outperforming existing methods without specialized equipment @plosbiology.org 🧪 plos.io/3XUsleH

Left: Graphic summary of the USPPAR approach. The entire process is divided into three stages. Dissociation involves separating whole cells from culture dishes or extracting nuclei from tissues, followed by fixation. Barcoding includes converting mRNA to cDNA with initial tagging, then performing 2–3 additional rounds of barcoding through ligation. Amplification encompasses cell/nucleus lysis, high-efficiency poly(dA) extension of cDNA, preamplification, tagmentation, and final library amplification. Right: Joint UMAPs of 50,584 murine hepatic nuclei, including 12,646 nuclei from each technology: USPPAR_pH3 (pH3 lysis), USPPAR_CuC (CuC lysis), Liver Cell Atlas (LCA, 10× Chromium), and PanSci (EasySci platform). Nuclei are colored by technology (Technology)

Single-cell RNA-sequencing based on combinatorial barcoding is pricey and lacks sensitivity. USPPAR is a novel #scRNAseq approach that combines split-pool #barcoding & optimized polydeoxyadenylation, outperforming existing methods without specialized equipment @plosbiology.org 🧪 plos.io/3XUsleH

The hand of a researcher holding one of the grids containing samples for barcoding. Photo credits: Alena Sucháčková.

View of the stunning landscape in Mals (Italy), where the first field campaign of CA 10kLepGenomes was conducted this summer. Photo credits: Alena Sucháčková.

Moths resting over one of the UV-light traps set during the samplings conducted in Mals for the first field campaign of CA 10kLepGenomes (July 2025). Photo credits: Alena Sucháčková.

🎉 #Barcoding results from specimens collected in the Mals field-workshop are in — thank you Marko Mutanen!
🧬We confirmed IDs of tricky specimens (mostly microleps), adding 43 new species to @projectpsyche.bsky.social and identifying 14 that need recollection.

Thanks to all who took part!

and in supp data the logo of diat.barcode ;-) #diatoms

Taxonomic tree of Diat.barcode v12. Hierarchical relationships of families according to their parent taxonomical links are represented. Size of bubbles gives the number of sequences in the corresponding families. Colors correspond to Classes: purple: Mediophyceae, red: Bacillariophyceae, green: Coscinodiscophyceae, blue: Fragilariophyceae.

Ecological guild distribution in the phylogeny (based on rbcl). Black color corresponds to a sequence belonging to an ecological guild. From inner to outer circle: high-profile, low profile, motile, planktonic.

Ordination biplots for Mainland France obtained from symmetric co-correspondence analysis (sCoCA). Ecological guilds are displayed in the left panel and species in the right panel. To improve the interpretation of the plots, species are colored based on their family and polygons represent the sampling sites from which they were sampled, regardless of their identification.

Navicula photo F Rimet

Happy to share our new paper presenting anew version of diat.barcode reference #barcoding #dna #library for #diatoms
Gelis, Barry-Martinet, Briand, Chonova, Cochero, Gassiole, Kahlert, Karthick, Keck, Kelly, Kochoska, Mann, Nayak, Pfannkuchen, Servulo […]

[Original post on mastodon.social]

A molecular perspective on horticultural trade: lessons from India for international species authentication The global horticultural industry faces increasing challenges related to species authentication, regulatory compliance, and trade transparency. Misidentification of plant species can result in economic losses, compromised consumer safety, and breaches of the regulatory framework...

#OpenAccess - A #molecular perspective on #horticultural trade: lessons from #India for international species authentication.
To read this new #review article in JHSB, visit: doi.org/10.1080/1462...

#IPR #DNA #Barcoding

All @biogeneurope.bsky.social outputs to date, in one place. You just have to browse and choose. That is BGE's Knowledge Platform. Go take a look 👉https://biodiversitygenomics.eu/knowledge-platform/ #genomics #barcoding #genomes @ebpgenome.bsky.social @iboleurope.bsky.social @ergabiodiv.bsky.social

Really looking forward to #LivingData2025, starting tomorrow! Below is a summary of the @biogeneurope.bsky.social sessions. For those in Europe: the conference takes place is Colombia, so mind the times! @ebpgenome.bsky.social @ergabiodiv.bsky.social @iboleurope.bsky.social
#barcoding #Genomics

How to Use Bulk Barcode Labels and GS1 Digital Link Printing Learn how to easily print bulk barcode labels and GS1 Digital Links using Avery templates to streamline your inventory and product tracking process.

🚀 New demo video: Bulk item labeling + GS1 Digital Link barcodes made easy.
Watch: youtu.be/u1DdUoDOpCE
Try smarter labeling free → gearchain.io/barcode-labels
#BarcodeLabels #ItemLabeling #InventoryManagement #GearChain #GS1 #Barcoding

ERGA iBOL Europe Connections #8 - Bioinformatics: reassembling the book of life - Biodiversity Genomics Europe About the 'ERGA iBOL Europe Connections' series The European Reference Genome Atlas (ERGA) and the European node of the International Barcode of Life (iBOL Europe), two international communities of sc...

New @ergabiodiv.bsky.social - @iboleurope.bsky.social Connections blog post. The title: #Bioinformatics: reassembling the book of life. Dont miss it! 👉 biodiversitygenomics.eu/2025/10/09/e... @horizoneu.bsky.social #genomics #DNA #barcoding

Turn Inventory Chaos Into Order With These Expert Barcoding Strategies Managing inventory effectively is one of the biggest challenges businesses face. Disorganized storage areas, inaccurate stock counts, and inefficient tracking systems can lead to costly errors. These issues waste time and affect customer satisfaction when products are misplaced, delayed, or unavailable. Implementing a strategic barcoding system brings clarity and structure to inventory operations, transforming chaotic […] The post Turn Inventory Chaos Into Order With These Expert Barcoding Strategies first appeared on Flowster.

Turn Inventory Chaos Into Order With These Expert Barcoding Strategies: Managing inventory effectively is one of the biggest challenges businesses face. Disorganized storage areas, inaccurate stock counts, and inefficient… #InventoryManagement #Barcoding #SupplyChain #BusinessStrategies #Efficiency

Foto: Sakaila africana Manning & Holthuis, 1981, recolectado en Guinea-Bissau. Muñoz I, Serrano M (2025). Colección de Crustáceos Marinos (CRUST-IEOCD). Version 1.33. Instituto Español de Oceanografía. Centro Oceanográfico de Cádiz (CSIC). Juego de datos de presencia https://doi.org/10.15468/anztjy, acceso a través de GBIF.org el 2025-10-07. https://www.gbif.org/occurrence/2564780161

Actualización Juego de datos. Colección de CrustáceosMarinos (CRUST-IEOCD) (3538 registros). Instituto Español de Oceanografía. Centro Oceanográfico de Cádiz (CSIC). Licencia CC BY. GBIF.ES

La colección CRUST-IEOCD suma >8500 especímenes (98% Decapoda) de aguas africanas y españolas: campañas científicas desde 2005 y donaciones desde los años 50. Incluye galería de imágenes y base de ADN #barcoding:
▶️ doi.org/10.15468/anz... #CiteTheDOI
▶️ colecciones.gbif.es/public/showD... @gbif.org

We reviewed the use of long-read sequencing with both @nanoporetech.com and @pacbio.bsky.social data for analyses of eukaryote #biodiversity for environmental samples #eDNA and #barcoding, providing a complete guide for future applications #BiodiversityGenomics @sgn.one
doi.org/10.32942/X2J...

Biodiversity Genomics Europe: Meet the rare Mediterranean White spotted Yellow Wart Slug YouTube video by Biodiversity Genomics Europe (BGE)

📽️ Watch the video for highlights of this conversation: www.youtube.com/watch?v=6CeP...

#referencegenome #genome #genomereport #BGE #DNA #barcoding #genomesequencing #biodiversity
@irbio-ub.bsky.social ‬@cnag-eu.bsky.social @ub.edu

🧬It's time to Read the Slime!
#Slimemoulds are weird, wonderful, and still understudied fungi-like organisms. You can help understand them.
Join the new FunDive citizen science campaign to find and sequence them 👉 fun-dive.eu/en/get-invol...
#ReadTheSlime #CitizenScience #Barcoding #FunDive

🧬It's time to Read the Slime!
#Slimemoulds are weird, wonderful, and still understudied fungi-like organisms. You can help understand them.
Join the new FunDive citizen science campaign to find and sequence them 👉 fun-dive.eu/en/get-invol...
#ReadTheSlime #CitizenScience #Barcoding #FunDive

100%!🤬🤬🤬

..And next "compulsory #Barcoding" of the whole population!

#Fcuk-Em!

Lets #Barcode this bunch of worthless #PoliticalGrifters ..to keep em in check!

New preprint!
We mapped the clonal architecture of mouse and human intestine using DNA barcoding, revealing key species-specific differences. Lessons from mice don’t always translate to humans!
biorxiv.org/content/10.110…. #StemCell #Barcoding #CRISPR #pISC #TOB2 #LGR5 #ASCL2

How The Smithsonian Safeguards 148 Million Specimens | AI in Action | Business Insider YouTube video by Business Insider

A great video highlighting the importance of collections at the Smithsonian USNM featuring Andrea Quattrini of @corallab.bsky.social Chris Meyer, Matt Buffington of Entomology and more! #barcoding #taxonomy #taxonomy youtu.be/KqSBoFXee2M?...

Morfología externa de los adultos de la especie de polilla geometridae, Isturgia pulinda deerraria (Geometridae, Ennominae, Macariini) (I. pulinda (Walker, 1860) ssp. deerraria (Walker, 1861)) en Senegal [(a) hembra adulta (© H. Jourdan); (b) macho adulto emergente (© A. Diop)] y Malta [(c) hembra adulta (© A. Catania); (d) macho adulto (© A. Catania)]

Mapa de Senegal que muestra el trazado de la Gran Muralla Verde en Senegal y los cinco sitios de muestreo con el perímetro del tipo de entorno muestreado

Identificada por primera vez en Senegal la polilla 𝐼𝑠𝑡𝑢𝑟𝑔𝑖𝑎 𝑝𝑢𝑙𝑖𝑛𝑑𝑎 𝑑𝑒𝑒𝑟𝑟𝑎𝑟𝑖𝑎, que defolia las acacias en la "Gran Muralla Verde" y dificulta la #reforestación contra la #desertificación. En el estudio se usó #barcoding de ADN y datos de @gbif.org:
▶️ doi.org/10.1007/s126... #CiteTheDOI 👇

Monitoring Methods for the Protection of Connectivity in Forest Ecosystems Forest ecosystems face increasing threats from climate change, resource exploitation, and other anthropogenic disturbances causing biodiversity loss and habitat fragmentation. The conservation priorit...

🌲🌳🌲🌳 Out now! #eDNA #barcoding #metabarcoding and the potential of #DNA-based methods for "Monitoring #Connectivity in #Forest #Ecosystems" doi.org/10.1007/978-... A fabulous collaboration with J. Oettel, A. Szukala, L. Munishi, K. Lapin and the #BFW #Austria @uniinnsbruck.bsky.social

This is the web presence for IOS, a Special Topic Network funded by the European Society of Evolutionary Biology. The network’s main goal is to contribute toward the integration of speciation research. Browse this site for more information on the IOS, its goals, and upcoming information about network activities.

👉What: “How could we use speciation theory to integrate DNA barcoding into taxonomy?”

👉More about the network: speciation-network.pages.ist.ac.at

horizoneu.bsky.social

#Biodiversity #Genomics #Europe #DNA #eDNA #barcoding #genomesequencing #biodiversity #speciation #taxonomy

Part of an email received informing me that my proposal has been funded in the GAUK grant competition. The title of the grant is in Czech: "Mechanismy diverzifikace blanokřídlých parazitoidů (Hymenoptera: Chalcidoidea) hálkotvorných organismů podél latitudy".

Woohoo! After an anxious 4 month wait from when I submitted my proposal to the GAUK grant competition, I got news that my PhD project will be getting funded 💰, particularly for UCE sequencing and #barcoding. I know a cryptic species complex hates to see us coming fr 😤

What brings us together? The European Reference Genome Atlas (ERGA) and the European node of the International Barcode of Life (iBOL Europe), two international communities of scientists brought together under the Biodiversity...

What connects #ERGA and @iboleurope.bsky.social?
In the 3rd edition of #Connections, we explore the commonalities between the #barcoding and #genome sequencing workflows 🧬

🔗 Read more: www.erga-biodiversity.eu/post/meet-ib... @biogeneurope.bsky.social

The first group of our #FunDive #Barcoding Campaign finished their work! It was a busy three days, as 18 amazing people from 7 countries prepared 192 samples for #Nanopore sequencing! Every step was registered on the #PlutoF platform. We hope to publish the sequencing results soon!

Universal RNA barcoding system for tracking gene transfer in bacteria created In the microscopic world of bacteria, gene transfer is a powerful mechanism that can alter cellular function, drive antibiotic resistance and even shape entire ecosystems. Now an interdisciplinary gro...

Universal #RNA #barcoding system for tracking #gene_transfer in #bacteria created.

phys.org/news/2025-03...

Biodiversity Genomics Europe: Alpine chamois reference genome boosts science-informed management YouTube video by Biodiversity Genomics Europe (BGE)

📽️ Watch the video for highlights of this conversation: www.youtube.com/watch?v=NM9-...

#referencegenome #genome #genomereport #BGE #DNA #barcoding #genomesequencing #biodiversity

DNA #barcoding relies on short #DNA sequences to identify species. With a broad network of more than 500 members across 29 countries, @iboleurope.bsky.social
is building a system to detect and monitor species, offering critical insights into their distributions and interactions.
💡 iboleurope.org

Use of massive DNA barcoding to monitor biodiversity: a test on forest soil fauna Biodiversity monitoring primarily focuses on particular taxonomic groups for which identification expertise is widely available, such as vascular plants or birds. As a result, the response of many tax...

New Preprint from my lab: #urep #DNA #Barcoding #mmm

www.biorxiv.org/content/10.1...