#Aging

Thoracic Aortic Stiffening (PWV by 9 mo) preceding Cardiac Hypertrophy (by 12 mo) in ♂️C57Bl/6🐭

👉Ex vivo passive aortic stiffness⬆️by 12 mo
👉Contraction-induced stiffening⬆️by 6 mo

Vasorelaxation to acetylcholine (EC function) declines mildly

#CommsBio 2022
www.nature.com/articles/s42...

hiPSC-derived Blood Vessel Organoid to model Moyamoya disease

Serum Proteomics 40 case + 20 control➡️
Multiple tubulin proteins⬆️ (TUBA1A TUBA4A TUBB1 TUBB5 TUBB4B)

Recap in Moyamoya organoids

TUBA4A or TUBB4B➡️
Connexin43➡️
KLF4➡️
⏫SMC prolifer/migration

#CommsBio 2026
www.nature.com/articles/s42...

#DiabeticCardiomyopathy #FGF9

FGF9⬇️in db/db 🐭🫀

AAV9-cTnt-FGF9 in db/db🐭
⏫🫀function LVEF LVFS
⏬Myocyte hypertrophy
⏬Myocyte lipid droplet
⏬Interstitial fibrosis
Abrogated by AMPKα2 T172A (domi neg)

FGF9-autophagy-p62-KEAP1-NRF2 cardioprotective axis

#CommsBio 2025
www.nature.com/articles/s42...

#VascularCalcification #SmoothMuscleCell m6A Epitranscriptomics

High Pi ⏬METTL3 in SMCs

METTL3-YTHDF2 destabilizes RUNX2 mRNA & ⏬ SMC Osteogenesis

S-Adenosylhomocysteine METTL3/14i aggravates aortic calcification in CKD 🐭 (adenine + hi Pi diet)

#CommsBio 2025
www.nature.com/articles/s42...

#SmoothMuscleCell #Piezo1 Small intestinal motility

SMC Piezo1 KO in 4~6 wk-old🐭
👉Muscularis thinning
👉⏬Food consumption
👉⏬SMC density w/o⏫apoptosis
👉⏬small intestinal motility
👉⏬Muscularis Orai1/3 Trpc4 IP3R ⏫Cav1.2 Ano1
👉⏬carbachol-induced tension

#CommsBio 2025
www.nature.com/articles/s42...

An interesting #Piezo1 - NF-κB feedforward loop in Vibration-induced injury (Inflammation, Apoptosis) of arterial #SmoothMuscleCell

NF-κB transcriptionally activates Piezo1?

Vibration injury
Rat tail 125 Hz accel 49 m/s2
👤umbilical SMC 125 Hz 5.9 m/s2

#CommsBio 2025
www.nature.com/articles/s42...

Cyclo(Pro-Tyr) elicits conserved cellular damage in fungi by targeting the [H+]ATPase Pma1 in plasma membrane domains

#CommsBio from Diego Romero
with Daniel Petras, Antoine Loquet, Alicia Isabel Pérez Lorente

#Bacillusvelezensis

www.nature.com/articles/s42...

The physiology of plants in the context of space exploration

#CommsBio by Massimo Maffei, Raffaella Balestrini et al with Marta Del Bianco

www.nature.com/articles/s42...

Spatial Multi-Omics of mouse hindlimb #SkeletalMuscle (TA EDL SOL)

Serial sections
Transcriptomics TOMOseq
Metabolomics #ImagingMassSpec

Intriguing complementary distribution b/w NAD+ & Glutamate/Succinate/Glutamine/2-Oxoglutarate

#CommsBio 2024
www.nature.com/articles/s42...

#YAP/TAZ #HydrostaticPressure #EndothelialCell

Effects of Pressure (20 mmHg capillary, 55 mmHg arteriole) on 2D & 3D EC behaviors
⏫#SproutingAngiogenesis
mouse aortic ring
3D EC microchannel
YAP/TAZ-dependent

⏫Partial #EndMT

⏫YAP nucleation

#CommsBio 2024
www.nature.com/articles/s42...

#Piezo1 at CD31-VE-cadherin intercellular junctions🤠

HA-tagged Piezo1 localized at CD31-VE-cad junctions in retinal vein

CD31 or VE-cad (but not VEGFR2)⏫junctional enrichment of HA-Piezo1 (FLIM COS-7)

CD31 OE⏬Piezo1-mediated Ca2+ flux (HUVEC)

#CommsBio 2024
www.nature.com/articles/s42...

#Angiogenesis-#Osteogenesis coupling orchestrated by #EndothelialCell SMAD1/5/ signaling
#SciArt😍

Induced EC Smad1/5 KO in 🐭P21 onwards->
⏬Tibial metaphyseal vessel arches
Depleted perivascular OSX+ osteoprogenitors
Expanded hypertrophic zone

#CommsBio 2024
www.nature.com/articles/s42...

Integrative analysis of yeast colony growth

#CommsBio from Attila Csikasz-Nagy

www.nature.com/articles/s42...

Genome-wide #AlternativeSplicing variation in #SmoothMuscleCell phenotypic plasticity

#Nanopore long-read RNAseq

Cultured👤aortic SMC
TGFβ/PDGFBB/miR-221

When your targets don't vary at the gene level, take a look at their AS variants

#CommsBio 2023
www.nature.com/articles/s42...