About the special collection (1/3)

This collection focuses on molecular to cell-level #mechanisms that drive #contractility in striated muscle. This area of research is transforming from science inspired primarily by curiosity into an active frontier where new results can have clinical impact.

Guidelines for assessing ventricular pressure-volume relationships in rodents | American Journal of Physiology-Heart and Circulatory Physiology Ventricular catheterization with a pressure-volume (PV) catheter is the gold-standard method for assessing in vivo cardiac function in animal studies, providing valuable “load-independent” indices of systolic and diastolic heart performance. PV studies are commonly performed to elucidate mechanistic insights into cardiovascular disease using surgical and genetically engineered rat and mouse models, but there is considerable heterogeneity in how these studies are performed. Wide variation in protocol design, volume calibration, anesthesia, manipulation of cardiac loading conditions, how load-independent indices of cardiac function are derived, as well as in data analysis and reporting is constraining reliability and reproducibility in the field. The purpose of this manuscript is to combine our collective expertise in performing open- and closed-chest left and right ventricle PV studies in rodents to provide consensus guidelines on how to perform, analyze, and interpret these studies using either conductance or admittance PV catheters. We first review recent methodological reporting in rodent PV studies in this journal and discuss important details required to improve reproducibility within and across PV studies. We then recommend steps to obtain high-quality PV data, from volume calibration to choice of anesthetic agent and acquiring load-independent indices of both systolic and diastolic function. We also consider between- and within-animal variation and recommend how to perform data analysis and visualization. We hope that this consensus paper guides those performing PV studies in rodents and helps align the field with best practices in surgical/analytical methodologies and reporting, facilitating better reliability and reproducibility in the PV field.

Kickstart 2025 with these new Guidelines for Assessing Ventricular Pressure-Volume Relationships in Rodents ow.ly/gqmN50UALLc
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#Contractility #PVloop #Guidelines #VentricularPressure #AJPHeart&Circ

Muscle contractile properties and perceived fatigue in the general and diseased population Knowledge of muscle contractile properties, physical fitness, and their associations with perceived fatigue may provide insights into mechanisms inducing fatigue and treatment targets. We aimed to id...

Muscle function and perceptions of #fatigue: Differences in @muscle contraction and physical fitness were examined in healthy or disease cohorts - findings include lower #contractility in patients with #COPD and higher perceived fatigue in #CML
doi.org/10.14814/phy...
@physoc.bsky.social
#MyoBlue

Development of an in vitro platform for the analysis of contractile and calcium dynamics in single human myotubes In vitro myotube cultures are widely used as models for studying muscle pathophysiology, but their limited maturation and heterogeneity pose significant challenges for functional analyses. While they ...

📢 Check out our recent work published in Lab on a Chip, describing a novel platform for the measurement of #contractility and #calcium dinamics in single human #myotubes with low variability and high reproducibility
👉 doi.org/10.1039/D3LC...
#myoblue 🧪