❓Tech Q&A | KG-501 inhibits the CREB-CBP interaction mainly via steric hindrance, disrupting CREB binding to CBP
📃Reported changes in CREB expression accompanying #AldoA levels suggest association, not causation (PMID: 15585582; 29290976)
#KG501 #StericHindrance #LabTips #Mechanisms

❓Tech Q&A | KG-501 inhibits the CREB-CBP interaction mainly via steric hindrance, disrupting CREB binding to CBP
📃Reported changes in CREB expression accompanying #AldoA levels suggest association, not causation (PMID: 15585582; 29290976)
#KG501 #StericHindrance #LabTips #Mechanisms

❓Tech Q&A | KG-501 inhibits the CREB-CBP interaction mainly via steric hindrance, disrupting CREB binding to CBP
📃Reported changes in CREB expression accompanying #AldoA levels suggest association, not causation (PMID: 15585582; 29290976)
#KG501 #StericHindrance #LabTips #Mechanisms

❓Tech Q&A | Use 10 μM for 5-30 min (commonly 20 min) at 37°C.
🧪Dilute 1:1000 in serum-free medium, cover #cells, wash 3× with serum-free medium.
🔃Order matters: short (≤2 h) load probe➡️stimulate; long (≥6 h) stimulate➡️load #probe.
✅Include a positive control (e.g. #CCCP).
#ROS #LabTips #DCFHDA

🔬 🧪𝗜𝗼𝗻 𝗣𝗮𝗶𝗿 𝗖𝗵𝗿𝗼𝗺𝗮𝘁𝗼𝗴𝗿𝗮𝗽𝗵𝘆: 𝗔𝗹𝗸𝘆𝗹 𝗦𝘂𝗹𝗳𝗼𝗻𝗮𝘁𝗲𝘀 𝘃𝘀. 𝗣𝗲𝗿𝗰𝗵𝗹𝗼𝗿𝗮𝘁𝗲𝘀
Optimise your IPC results! Learn when to use alkyl sulfonates for retention control or perchlorates for suppressing tailing, based on their unique properties.

📖 𝗥𝗲𝗮𝗱 𝗺𝗼𝗿𝗲: cutt.ly/7tqcpush

#Chromatography #Science #LabTips

Erlenmeyer Flask: Tips for Efficient Lab Use Optimize your lab procedures with the Erlenmeyer flask. Learn about its unique benefits and practical applications in chemical experiments.

Erlenmeyer flasks improve lab efficiency with safer mixing, heating, and culture growth. Learn key tips for smart lab use, now read our full blog content: blog.certifiedmtp.com/efficiency-w...
Essectial tools here: certifiedmtp.com/labware/

#LabTips #ErlenmeyerFlask #certifiedmtp #ChemistryTips

Mercury in Glass Thermometers: Dangers and Hazards Discover the history and hazards of mercury in glass thermometers, and explore safer alternatives for measuring temperature.

Mercury thermometers are accurate but risky if broken. Safer digital and mercury-free options exist. Good to know, especially around kids and labs.

Read immediately: blog.certifiedmtp.com/dangers-of-m...

Shop from here: certifiedmtp.com/glass-mercur...

#healthsafety #labtips #certifiedmtp

Microscopic view of clustered, brightly colored spherical cells with glowing orange and purple hues against a dark background.

Culturing organoids?
Avoid these pitfalls:

1️⃣ Skipping ECM optimization
2️⃣ Letting them get too big (necrosis!)
3️⃣ Using low-quality growth factors
4️⃣ Poor cryopreservation/thawing

More research workflow solutions here: ow.ly/IuvT50WZvyM

#Organoids #Proteintech #LabTips

🧪 Technique Thursday | Master your staining! New MedChemExpress guide walks you through IHC, ICC & IF techniques with workflows, antibody tips & troubleshooting.
👉 www.medchemexpress.com/literature/i...
#TechnicalThursday #IHC #ICC #IF #LabTips #DrugDiscovery

PCR doesn't have to be slow, expensive, or wasteful.
Small changes = big impact.

#PCR #LabTips #PhDLife #GreenLab #ScienceTwitter

mohr pipette

Most lab errors start with misusing pipettes. Learn Mohr pipette precision in minutes—accuracy starts here! 👉 blog.certifiedmtp.com/mohr-pipette...
Explore product: certifiedmtp.com/25ml-mohr-ty...

#LabEquipment #MohrPipette #LabTips #ScienceTools #LabAccuracy #PipettePrecision #CertifiedMTP

Graduated Pipette Use: Tips for Accurate Lab Measurements Enhance lab accuracy with graduated pipette tips. Ensure precise measurements to boost reliability and efficiency in scientific experiments.

Are your lab results accurate? One pipetting mistake can ruin everything. Master proper technique now 👉 Read Blog: blog.certifiedmtp.com/graduated-pi...
Shop here: certifiedmtp.com/labware/#d69...

#LabTips #GraduatedPipette #ScientificResearch #ScienceTools #PipettingTips #CertifiedMTP

ELISA Controls | Antibodies.com A detailed guide to ELISA controls, including controls for non-specific binding, wavelength, standards, and matrix interference

🔬 Running an ELISA? Controls matter.

From background correction to troubleshooting, the right ELISA controls ensure reliable results.

🧪 Learn more: www.antibodies.com/applications...

#ELISA #LabTips #Immunoassay #LifeSciences

Texture measurement: Optimising your sample preparation is key for accurate, reproducible results. Minimise variability and ensure that your measurements truly reflect material properties. Discover more in our blog post: bit.ly/42rt0Gg #TextureAnalysis #LabTips

6/ One More Tip
Be paranoid! PCR contamination is sneaky—good habits keep your results clean.
🔬 Got more PCR contamination tips? Drop them below! 👇 #PCR #MolecularBiology #LabTips

Another sign you lab a lot… #365DaysOfScience #labtips

Mass Spectrometry Blogs - MS Wil On this page we will provide you with our insights into the field of Mass Spectrometry by means of our blogs/tech notes.

💡 Did you know we’ve collected several Product Notes on our website?

Quick tips, applications, and insights to help you get the most out of our products!

Check them out 👉 www.mswil.com/blogs/

#MassSpec #Proteomics #LabTips #MSWil

Want to upgrade your immunofluorescence workflow? Go Direct! Directly conjugated antibodies are gaining popularity due to their ease and flexibility in staining multiple antigens in the same sample and offer multiple advantages when multiplexing.

Want to upgrade your #immunofluorescence workflow? Go direct!

Visit the #ProteintechBlog to learn about the advantages of using directly conjugated primary #antibodies for IF 🧪

5 min read: www.ptglab.com/news/blog/wa...

#PhDChatter #Immunoassay #LabTips

When you have a small amount to weigh out, don’t worry about being exact. Instead, it’s easier to change the volume to match what you weigh to get your desired concentration. more about concentrations & making solutions: bit.ly/sciencestock... #365DaysOfScience #labtips #biochemsky #chemsky 🧪

Quick lab tips: Stir bars and solution pouring (without stir bar pouring) YouTube video by the bumbling biochemist

It's always fun getting to teach students lab tricks: today, how you can hold a large magnetic stir bar on the outside of your magnetic stir bar containing beaker to prevent the stir bar from falling in #365DaysOfScience #labtips #biochemsky #chemsky

youtube.com/shorts/oQ9p4...

We've gathered some fantastic kindness tips that our team has shared with us from the lab. ❤️ #bekind #labtips #kindness

TLC Troubleshooting with SiliCycle's SiliaPlate SiliCycle provides a variety of TLC plates in different sizes and chemistries, ensuring high separation power. Discover practical tips to optimize your TLC separation techniques.

Having trouble with Thin Layer Chromatography (TLC)? 🧪
Discover how to optimize your separations with SiliCycle's expert tips! From solvents to visualization, this guide covers it all.

Check out our Application Note:
www.silicycle.com/resource-cen...

#chemsky #TLC #chromatography #labtips

Mounting your Pencil Heater for IonOpticks Aurora Rapid75 (5cm) columns on a Thermo Fisher Scientific Ion Source - MS Wil Over the years MS Wil has provided many Proteomics laboratories with solutions to build column ovens close to the orifice of the Mass Spectrometer. Outside bespoke solution from Sonation, these ovens ...

"Struggling to mount your pencil heater for IonOpticks Aurora Rapid75 5cm columns on a Thermo Fisher Scientific ion source? Our guide makes it simple! Find step-by-step instructions for perfect setup here: www.mswil.com/mounting-you...

🔬 #MSWil #IonOpticks #LabTips #MassSpec"

Hint: Cleaning a phenol-contaminated centrifuge is not exactly a fun adventure. Avoid the hassle by taking good care when working with phenol-chloroform. Trust me, your labmates will thank you. #labtips #protocolsio 5/5